Genetic shifts in methicillin-resistant Staphylococcus aureus epidemic clones and toxin gene profiles in Japan: comparative analysis among pre-epidemic, epidemic and post-epidemic phases

Osaka, Shunsuke; Okuzumi, Katsuko; Koide, Shota; Tamai, Kazuya; Sato, Tomoaki; Tanimoto, Koichi; Tomita, Haruyoshi; Suzuki, Masahiro; Nagano, Yukiko; Shibayama, Keigo; Arakawa, Yoshichika; Nagano, Noriyuki

doi:10.1099/jmm.0.000687

Cited by 17 publications

(14 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results suggest that the eight analyzed sequences belong to five distinct CFs. CF is a term that refers to the smallest unit of diferenciation in prokaryotes, (also referred to as strain biotypes or serotypes) and equivalent to the concept of “epidemic clone” used in the clinical field ( Cui et al, 2015 ; López-Pérez and Rodriguez-Valera, 2016 ; Osaka et al, 2018 ). Strains within the same CF showed lower ANIb and dN/dS pairwise distances as compared to strains from different CFs ( Figure 3 ), which is in line with previous studies in which HR was shown to act as the main evolutionary mechanism in highly recombinogenic bacteria ( Castillo-Ramírez et al, 2011 ; Cadillo-Quiroz et al, 2012 ).…”

Section: Resultsmentioning

confidence: 99%

Genome Variation in the Model Halophilic Bacterium Salinibacter ruber

González-Torres

Gabaldón

2018

Front. Microbiol.

View full text Add to dashboard Cite

The halophilic bacterium Salinibacter ruber is an abundant and ecologically important member of halophilic communities worldwide. Given its broad distribution and high intraspecific genetic diversity, S. ruber is considered one of the main models for ecological and evolutionary studies of bacterial adaptation to hypersaline environments. However, current insights on the genomic diversity of this species is limited to the comparison of the genomes of two co-isolated strains. Here, we present a comparative genomic analysis of eight S. ruber strains isolated at two different time points in each of two different Mediterranean solar salterns. Our results show an open pangenome with contrasting evolutionary patterns in the core and accessory genomes. We found that the core genome is shaped by extensive homologous recombination (HR), which results in limited sequence variation within population clusters. In contrast, the accessory genome is modulated by horizontal gene transfer (HGT), with genomic islands and plasmids acting as gateways to the rest of the genome. In addition, both types of genetic exchange are modulated by restriction and modification (RM) or CRISPR-Cas systems. Finally, genes differentially impacted by such processes reveal functional processes potentially relevant for environmental interactions and adaptation to extremophilic conditions. Altogether, our results support scenarios that conciliate “Neutral” and “Constant Diversity” models of bacterial evolution.

show abstract

Section: Resultsmentioning

confidence: 99%

Genome Variation in the Model Halophilic Bacterium Salinibacter ruber

González-Torres

Gabaldón

2018

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The World Health Organization states that antimicrobial resistance is a global problem due to the increasing incidence of infections with priority pathogens and persisting mortality, despite the introduction of new antibiotics [ 91 ]. We include the following bacteria in the above group of microorganisms constituting a danger for social health: Enterococcus faecium , Staphylococcus aureus , Klebsiella pneumoniae , Acinetobacter baumannii , and Pseudomonas aeruginosa , collectively referred to by the acronym ESKAPE [ 88 , 92 , 93 ]. Infections with bacteria belonging to the Enterobacteriaceae family are the main cause of morbidity and death in the USA and EU countries [ 88 ].…”

Section: The Use Of Ionic Liquids In Microbiology and Medicinementioning

confidence: 99%

“…The described mechanisms lead to antibiotic resistance to penicillins, cephalosporins, monobactams, and carbapenems, and there is an additional phenomenon of cross-resistance to macrolides and fluoroquinolones. ILs used in the treatment of general and local infections are not susceptible to either a antibiotic-resistant mechanism, which was proven in the studies on the SA1199 A and B staphylococcal strains (see Table 2 ) [ 86 , 91 , 92 , 93 ].…”

Section: The Use Of Ionic Liquids In Microbiology and Medicinementioning

confidence: 99%

See 1 more Smart Citation

The Use of Liquids Ionic Fluids as Pharmaceutically Active Substances Helpful in Combating Nosocomial Infections Induced by Klebsiella Pneumoniae New Delhi Strain, Acinetobacter Baumannii and Enterococcus Species

Miskiewicz

Ceranowicz

Szymczak

et al. 2018

IJMS

View full text Add to dashboard Cite

This review deals with various microbiological activities of ionic liquids, which constitute the first anti-infective defense against multi-drug-resistant bacteria—with a particular emphasis placed on medicine and pharmacology. The quoted data on the biological activity of ionic liquids including their antimicrobial properties (depending on the type of a cation or an anion) and are discussed in view of possible applications in nosocomial infections. Dedicated attention is given to finding infections with the Klebsiella pneumoniae New Delhi strain, Acinetobacter baumannii, and Enterococcus species, which are responsible for the induction of antibiotic resistance in intensive care units. Diagnosis and treatment using current antibiotics is a significant problem in hospital care, and the relevant burden on the health systems of the European Union member states induces the search for new, effective methods of treatment. Ionic liquids, due to their antibacterial effect, can be considered topical and general medications and may provide the basis for treatment to eliminate the antibiotic resistance phenomenon in the future. At present, the number of infections with resistant pathogens in hospitals and outpatient clinics in the European Union is growing. In 2015–2017, a significant incidence of respiratory and bloodstream infections with bacteria resistant to antibiotics from the 3rd generation group of cephalosporins, glycopeptides, and carbapenems were observed. The paper presents examples of synthesized bifunctional salts with at least one pharmaceutically active ion in obtaining a controlled release, controlled delivery, and biological impact on the pathogenic bacteria, viruses and fungi. The ionic liquids obtained in the presented way may find applications in the treatment of wounds and infections.

show abstract

“…In the past few decades, the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) infection has changed from a hospital to a community setting. Since the 1980’s, the spread of MRSA infections has been accompanied by healthcare-associated setting, but decreasing trend has been observed since 2008 in Japan ( 1 ). By contrast, community-associated infections have been rising the frequency since 1990’s around the world ( 2 , 3 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of healthcare and community-associated methicillin-resistant Staphylococcus aureus using phage open-reading frame typing

Konuma¹,

Takahashi²,

Suzuki³

et al. 2021

IJM

View full text Add to dashboard Cite

The polymerase chain reaction-based open reading frame typing (POT) method is a simple and rapid method for the strain-level discrimination of methicillin-resistant Staphylococcus aureus (MRSA). We investigated the molecular charac- teristics of S. aureus strains by multilocus sequencing typing (MLST) and POT and the profiles of antibiotic resistance and virulence genes of MRSA isolates in a single center of Tokyo, Japan. Five types by MLST and 19 types by POT were detected in the 25 MRSA isolates. ST5 and a POT1 score of 93 were associated with healthcare-associated MRSA, whereas ST8 and a POT1 score of 106 were associated with community-associated MRSA. Each strain evaluated by POT score was completely associated with similar profiles of antibiotic resistance and virulence genes. These data showed that the POT system was a powerful molecular tool for the epidemiological characterization of MRSA isolates, which correlated with the profiles of antibiotic resistance and virulence genes.

show abstract

Genetic shifts in methicillin-resistant Staphylococcus aureus epidemic clones and toxin gene profiles in Japan: comparative analysis among pre-epidemic, epidemic and post-epidemic phases

Abstract: Over time, a transition in MRSA genetic profiles from a POT1 score of 93 in 1990 and 2004 to 106 in 2014 was found in Japan.

Cited by 17 publications

References 24 publications

Genome Variation in the Model Halophilic Bacterium Salinibacter ruber

Genome Variation in the Model Halophilic Bacterium Salinibacter ruber

The Use of Liquids Ionic Fluids as Pharmaceutically Active Substances Helpful in Combating Nosocomial Infections Induced by Klebsiella Pneumoniae New Delhi Strain, Acinetobacter Baumannii and Enterococcus Species

Molecular characterization of healthcare and community-associated methicillin-resistant Staphylococcus aureus using phage open-reading frame typing

Contact Info

Product

Resources

About