Genetic survey of loggerhead turtle Caretta caretta nesting population in Tunisia

Chaieb, Olfa; Ouaer, Ali El; Maffucci, Fulvio; Bradaï, Mohamed Nejmeddine; Bentivegna, Flegra; Saïd, Khaled; Chatti, Noureddine

doi:10.1017/s175526721000014x

Cited by 13 publications

(11 citation statements)

References 18 publications

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“…Genetic differentiation between the 2 Libyan nesting beaches was verified with the Exact test of population differentiation (Raymond & Rousset 1995), computed with 100 000 steps in the Markov Chain with 10 000 dememorization steps, and pairwise Φ st (Excoffier et al 1992) computed with 10 000 random permutations. The same statistical analysis was used to compare Libyan rookeries to the other Mediterranean nesting populations for which genetic data were available , Laurent et al 1998, Garofalo et al 2009, Chaieb et al 2010; Table 1). Sequential Bonferroni correction was not applied because it may increase the likelihood of Type II error for multiple pairwise comparisons (Moran 2003).…”

Section: Discussionmentioning

confidence: 99%

Loggerhead turtles nesting in Libya: an important management unit for the Mediterranean stock

Saied¹,

Maffucci²,

Hochscheid³

et al. 2012

Mar. Ecol. Prog. Ser.

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Section: Discussionmentioning

confidence: 99%

Loggerhead turtles nesting in Libya: an important management unit for the Mediterranean stock

Saied¹,

Maffucci²,

Hochscheid³

et al. 2012

Mar. Ecol. Prog. Ser.

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“…However, their conclusion was based on the analysis of just one nesting ground from the Ionian Sea (Bay of Kyparissia), the only rookery sampled at that time. New genetic data on the Mediterranean populations have come to light since (Carreras et al, 2007;Chaieb et al, 2010;Encalada et al, 1998;Garofalo et al, 2009;Laurent et al, 1998;Saied et al, 2012;Yilmaz et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA reveals Pleistocenic colonisation of the Mediterranean by loggerhead turtles (Caretta caretta)

Clusa

Carreras

Pascual

et al. 2013

Journal of Experimental Marine Biology and Ecology

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As the loggerhead turtle (Caretta caretta) is a philopatric species with a strong genetic structure, the analysis of mtDNA can be used to track evolutionary and colonisation events. In this study we use a genetic approach to understand the population structure of C. caretta in the Mediterranean Sea and to test whether loggerheads could have colonised the Mediterranean during the Pleistocene and survived the cold phases in warm refugia. We amplified a long mtDNA D-loop fragment (815 bp) from 168 dead hatchlings sampled from a selection of rookeries in the Eastern Mediterranean: Libya, Israel, Lebanon, Cyprus and Greece. Previously published data from Turkey and Calabria (Southern Italy) were also included in the analyses. The population nesting in Libya emerged as the oldest population in the Mediterranean, dating from the Pleistocene ca. 65,000 years ago (20,000). This reveals that the Libyan population might have settled in the Mediterranean basin before the end of the last glacial period. The remaining nesting sites, except Calabria, were subsequently colonised as the population expanded. The populations nesting in Eastern Turkey and Western Greece settled ca. 30,000 years ago (10,000-100,000), whereas the remaining populations originated as a result of a more recent Holocenic expansion. As Calabria presented a unique Atlantic haplotype, found nowhere else in the Mediterranean, we consider this nesting site as the result of an independent colonisation event from the Atlantic and not the recent spread of Mediterranean populations. This reveals that the current genetic structure of C. caretta rookeries in the Mediterranean would be the result of at least two colonisation events from the Atlantic, the oldest one in Libya and a most recent in Calabria, combined with local extinctions during Pleistocenic glaciations and re-colonisations from glacial refugia in Libya, Eastern Turkey and Western Greece.

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“…A fine scale sampling effort within Turkey confirmed this hypothesis at a local level (Schroth et al 1996). The sequencing of a 380-bp fragment of the hyper-variable region of the D-loop of the mtDNA and the increasing of sampled locations has markedly improved the knowledge of Mediterranean genetic structure and different management units (Moritz 1994) were defined , Laurent et al 1998, Chaieb et al 2010. A recently designed set of primers (AbreuGrobois et al 2006) notably increased the length of the fragment sequenced, allowing enhanced description of management units, inter-population connections and genetic barriers within the Mediterranean (Yilmaz et al 2011, Saied et al 2012, Clusa et al 2013.…”

Section: Introductionmentioning

confidence: 74%

“…Our statistical power analysis clearly shows that sample sizes below 20 might fail when detecting differentiation and thus higher numbers are desirable, and this could explain why all but one non-significant value involve one of the two populations with the lowest sample size (Misurata and Lebannon). Many genetic studies have been conducted in the Mediterranean nesting beaches over the last two decades , Laurent et al 1998, Garofalo et al 2009, Chaieb et al 2010, Yilmaz et al 2011, Saied et al 2012, Clusa et al 2013, sometimes resulting in the production of several sample sets for the same locations. The risk of pseudoreplication between different sample sets is very high, especially in view of the small population sizes of some of the nesting beaches.…”

Section: Discussionmentioning

confidence: 99%

“…We amplified a long (~800 bp) fragment of the mitochondrial DNA (mtDNA) control region using the primers LCM15382 (5'-GCTTAACCCTAAA-GCATTGG-3') and H950 (5'-GTCTCGGATT-TAGGGGTTT-3') (Abreu- Grobois et al 2006), which includes the short region (~380 bp) historically surveyed for several marine turtle species in previous studies , Laurent et al 1998, Chaieb et al 2010. Extraction and amplification were conducted using the Phire ® Animal Tissue Direct PCR Kit (Finnzymes) following the manufacturer's specifications.…”

Section: Methodsmentioning

confidence: 99%

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Mitochondrial DNA markers of loggerhead marine turtles (<em>Caretta caretta</em>) (Testudines: Cheloniidae) nesting at Kyparissia Bay, Greece, confirm the western Greece unit and regional structuring

Carreras¹,

Rees²,

Broderick³

et al. 2014

Sci. Mar.

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Summary: Genetic markers have been widely used in marine turtles to assess population structuring and origin of individuals in common feeding grounds, which are key elements for understanding their ecology and for developing conservation strategies. However, these analyses are very sensitive to missing information, especially from abundant nesting sites. Kyparissia Bay (western Greece) hosts the second largest Mediterranean nesting aggregation of the loggerhead turtle (Caretta caretta), but the genetic profile of this nesting site has not, as yet, been described using the extended version of the historically used mitochondrial DNA (mtDNA) marker. This marker was genotyped for 36 individuals nesting at Kyparissia Bay and haplotype frequencies obtained were compared with published data from other Mediterranean nesting sites. The results confirmed the connection between Kyparissia and other western Greek nesting sites and the isolation of this western Greek group from other Mediterranean nesting areas. As a consequence of this isolation, this abundant group of nesting aggregations (almost 30% of the Mediterranean stock) is not likely to significantly contribute to the recovery of other declining Mediterranean units.Keywords: population structure; endangered species; conservation; Caretta; Mediterranean; mitochondrial DNA.El uso de marcadores de ADN mitocondrial en tortuga boba (Caretta caretta) (Testudines: Cheloniidae) nidificando en la bahía de Kyparissia, Grecia, confirma la unidad de Grecia oriental y la estructuración regional Resumen: El uso de marcadores genéticos ha sido muy extendido en tortugas marinas con el fin de determinar la estructura poblacional y el origen de individuos en zonas comunes de alimentación; elementos clave para entender su ecología y desarrollar estrategias efectivas de conservación. Sin embargo, este tipo de análisis es muy sensible a la falta de información de ciertas zonas de nidificación, especialmente de aquellas muy abundantes. El perfil genético de las tortugas nidificantes de la bahía de Kyparissia (Grecia occidental) todavía no ha sido descrito usando la versión extendida del marcador mitocondrial (mtDNA) usado históricamente en esta especie, a pesar de ser la segunda zona de nidificación más abundante de todo el Mediterráneo. Con el fin de cubrir este vacío de información, se secuenciaron 36 individuos nidificantes de la bahía de Kyparissia y se compararon las frecuencias de haplotipos obtenidas con datos publicados de otras zonas de nidificación del Mediterráneo. Los resultados confirmaron la conexión entre Kyparissia y otras zonas de nidificación de Grecia así como el aislamiento de este grupo de Grecia occidental con el resto de zonas de nidificación del Mediterráneo. Como consecuencia de este aislamiento, todo parece indicar que este abundante grupo de zonas de nidificación (casi el 30% de la producción del Mediterráneo) no podría contribuir de forma significativa a la recuperación del número de hembras nidificantes en otras poblaciones en declive del Mediterráneo.Palabr...

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Genetic survey of loggerhead turtle Caretta caretta nesting population in Tunisia

Cited by 13 publications

References 18 publications

Loggerhead turtles nesting in Libya: an important management unit for the Mediterranean stock

Loggerhead turtles nesting in Libya: an important management unit for the Mediterranean stock

Mitochondrial DNA reveals Pleistocenic colonisation of the Mediterranean by loggerhead turtles (Caretta caretta)

Mitochondrial DNA markers of loggerhead marine turtles (<em>Caretta caretta</em>) (Testudines: Cheloniidae) nesting at Kyparissia Bay, Greece, confirm the western Greece unit and regional structuring

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