Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers

Poerba, Yuyu Suryasari; Ahmad, Fajarudin

doi:10.13057/biodiv/d110303

Cited by 19 publications

(21 citation statements)

References 28 publications

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“…There was a close relationship between some of the plantain ecotypes used in this study, as these ecotypes may have been derived from the same pedigree or the amplification of the same nucleotide sequence present between the simple sequence repeats. Similar results have been found by Poerba and Ahmad (2010) for 36 triploid banana accessions with an estimated 46% genetic similarity based on RAPD and ISSR marker data. ISSR markers have also been used for genetic diversity analysis in Musa spp.…”

Section: Discussionsupporting

confidence: 86%

Molecular variability of plantain ecotypes from the genus Musa (Musaceae)

Choudhary¹,

Keshavachandran²,

Menon³

et al. 2014

Turk J Bot

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IntroductionBanana (Musa L. species (Musaceae)) is considered as an important food-fruit crop and is cultivated commercially in more than 120 countries. India is a major banana producing nation with an area of 0.79 million hectares and productivity of 28.4 metric tonnes per hectare (NHB, 2011). A wide range of diploids, triploids (2n = 3x = 33), and tetraploid cultivars/landraces of banana are known that have evolved from interspecific hybrids of the 2 wild diploid species, Musa acuminate Colla (contributing ' A' genome) and Musa balbisiana Colla (contributing 'B' genome). Despite botanical homogeneity, plantains manifest wide and unique phenotypic variability regarding plant size, bunch type, bunch and fruit orientation, fruit apex shape, pseudostem, and fruit color. Nendran (Musa AAB group) is one of the leading banana cultivars belonging to the plantain subgroup and is one of the most prized plantain varieties of India. However, biodiversity in the Nendran cultivar is complex and is represented by clones distinguishable by variation in plant stature, bunch, fruit morphology, and degree of development of the male phase (Menon et al., 2002). Therefore, the present study was carried out with the objective to characterize variability in plantain ecotypes of banana using molecular markers. The development and application of molecular markers provide powerful tools to reveal polymorphism, are robust to detect genetic variability (Simmons et al., 2007), and are not influenced by environment or developmental stages of the plant, thus making them an ideal tool for genetic relationship studies. However, the potential usefulness of Abstract: Twelve plantain ecotypes were characterized for molecular variability using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Out of the 60 RAPD and 40 ISSR primers tested, 16 RAPD and 14 ISSR primers were found polymorphic, and were used for molecular profiling of the plantain ecotypes. The average number of bands per primer, average number of polymorphic bands per primer, mean polymorphic information content (PIC) value, resolving power (Rp), and marker indices (MI) for RAPD primers were 8.63, 3.25, 0.87, 15.49, and 2.86, whereas the respective values for the ISSR assay were 7.93, 4.14, 0.85, 12.49, and 3.55. The genetic similarity coefficient was calculated using the Jaccard coefficient. The unweighted pair group method with arithmetic averages (UPGMA)-based clustering pattern remained more or less similar for RAPD, ISSR, and combined RAPD and ISSR data. Clustering was strongly supported by high bootstrap values. The genotypes Njockkon and Changalikodan (similarity coefficient > 0.94) and Manjeri Nendran (a) and Manjeri Nendran (b) (similarity coefficient > 0.89) were more closely related than the other ecotypes. The principal component analysis (PCA) showed results similar to those of the dendrogram. The results revealed that ISSR would be a better tool than RAPD for evaluation of genetic diversity in plantain ecotypes. Further, huge variab...

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Section: Discussionsupporting

confidence: 86%

Molecular variability of plantain ecotypes from the genus Musa (Musaceae)

Choudhary¹,

Keshavachandran²,

Menon³

et al. 2014

Turk J Bot

View full text Add to dashboard Cite

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“…This is similar to report by Poerba and Ahmad (2010) where a value of 0.06 was observed between two accessions in their collection. Resmi et al (2011) used ten STMS primers to detect polymorphism among 38 Indian Musa cultivars, and this revealed 27 alleles.…”

Section: Discussionsupporting

confidence: 92%

“…The advantage being, no prior information of genetic make-up is needed. Poerba and Ahmad (2010) used five RAPDs and ISSR to characterize 36 accessions of cooking banana. The five random primers used generated 63 bands.…”

Section: Discussionmentioning

confidence: 99%

“…All the bands generated were polymorphic. Unlike the reports by Poerba and Ahmad (2010) as well as Brown et al (2009), where AAB and ABB and other Musa genomes were used, in this particular study, mainly AAB genome Musa accessions were analysed, the analysis included FHIA 21, which is an introduced tetraploid Musa hybrid. This FHIA 21 clustered between two known genotypes in the dendogram, whereas in the PCA presentation, it formed a separate group.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Unravelling genetic make-up of some Musa hybrids and selected Musa accessions using molecular and morphological characterization

Quain¹,

Agyeman²,

Okyere³

et al. 2018

Int. J. Genet. Mol. Biol.

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Plantains are important staples in sub-Saharan Africa, however, production has been saddled by several constraints including pests and diseases and lack of improved technologies. In regard to genetic study, morphological characterization has identified about 100 local accessions to exist in Ghana. However, limited molecular characterization has been done on plantains. Characterization based on morphologic characteristics alone may be limited since expression of quantitative traits is subjective to strong environmental influence. Alternatively, molecular characterization techniques are capable of identifying polymorphism represented by differences in DNA sequences. The objective of this research was therefore to characterize local accessions of plantain using morphological and molecular techniques. This study sampled 38 local accessions from two regions in Ghana for characterization, taking into account all known morphological characteristics. Genomic DNA was isolated for molecular characterization using 18 SSR markers and 10 random primers. The SSR markers revealed 52 alleles, random primers revealed 67 alleles and the 29 morphological traits (loci) had 79 attributes (alleles). Similarity matrices of SSR markers revealed that 26.5% of the plantain accessions were duplicates; however, random amplified polymorphic DNA (RAPDs) system and morphological marker systems did not reveal any duplication among genotypes. Dendrogram generated on bases of both SSR and RAPDs indicated that a French plantain accession (Apem 01) and a False Horn accession (Apantu 26) were the most distantly related collections. None of the molecular marker methods used clearly clustered various phenotypically known collections into their respective distinct groups, although to some extent, this was demonstrated by morphological data set.

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“…Moreover, banana trends to be in high demand in global markets, expected to reach 18.1 million tons in 2017 (FAO, 2017).The edible and plantain banana cultivars resulted from hybridization of two wild cultivars of M. acuminata (AA genome) and M. balbisiana (BB genome). These cultivars are mostly seedless and classified into various polyploidy levels, according to their genome component; diploid (AA and BB), triploid (AAA, AAB, ABB, and BBB) and tetraploids(AAAA, AAAB, AABB, and ABBB) ( Kiran et al, 2015).Of these genomes, triploid genomes are widely cultivated and played a significant role in world economy and food security in the developing countries, especially in South East Asia, East and Central West Africa ( Poerba and Ahmad, 2010;Opara et al, 2010;Singh et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity among thai banana cultivars (Musaspp.) based on RAPD and SRAP markers

2018

Int. J. Biosci.

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This study was conducted to assess the genetic diversity and relationship of 19Thai Musa accessions of four (ABB, AAA, AA, and BB) genomes based on Random Amplified Polymorphic DNA (RAPD) and Sequence Related Amplified Polymorphism (SRAP) markers. The results revealed that thirteen RAPD and eight SRAP markers generated a total of 129 alleles (calculated from 1005 amplified fragments), with sizes ranging from 100 to 2100 bp long. Moreover, both markers provided high polymorphism with average PIC value of 0.75, which indicated a high level of genetic diversity among studied Musa accessions. The UPGMA approach based grouping showed two distinct clusters, which were cluster-I comprised of ABB, AA, and BB genomes and cluster-II consisted of only the AAA genome. Additionally, its analysis demonstrated high genetic relationship within the ABB genome, compared to other (AAA, AA, and BB) genomes. This study also confirmed that both RAPD and SRAP markers were proficient tools for rapidly accessing the genetic diversity within all the banana genomic groups.

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Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers

Cited by 19 publications

References 28 publications

Molecular variability of plantain ecotypes from the genus Musa (Musaceae)

Molecular variability of plantain ecotypes from the genus Musa (Musaceae)

Unravelling genetic make-up of some Musa hybrids and selected Musa accessions using molecular and morphological characterization

Assessment of genetic diversity among thai banana cultivars (Musaspp.) based on RAPD and SRAP markers

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