Genetic variability and aflatoxigenic potential of<i>Aspergillus flavus</i>isolates from maize

Karthikeyan, M.; Sandosskumar, R.; Mathiyazhagan, S.; Mohankumar, M.; Valluvaparidasan, V.; Kumar, Sachin; Velazhahan, R.

doi:10.1080/03235400600950961

Cited by 13 publications

(14 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lane; M-100 bp ladder been found to contain regions of variability within genera (White et al 1990;Seifert et al 1995;Levy et al 2001). The ITS region of nuclear ribosomal DNA, including ITS1, ITS2 and the intervening 5.8S rRNA gene, has been widely used to study the variability in fungi at the species and sub-species levels (Peterson 1991;Cooke et al 1996;Crawford et al 1996;Cooke and Duncan 1997;Goodwin and Zismann 2001;Appiah et al 2004;Karthikeyan et al 2009). Henry et al (2000) reported sequence variation in several areas in the ITS regions among referenced and clinical isolates of Aspergillus species.…”

Section: Resultsmentioning

confidence: 99%

“…was isolated from the collected samples using the potato dextrose agar (PDA) medium under laboratory conditions (Karthikeyan et al 2009). The Aspergillus cultures were identified up to the species level using a taxonomic key and species descriptions (Singh et al 1991).…”

Section: Isolation Of Aspergillus Sppmentioning

confidence: 99%

“…Hence, their quantity in food and feed is closely monitored and regulated in most countries (van Egmond 1995). Variability in aflatoxin production potential of A. flavus isolates have been reported (Karthikeyan et al 2009). This is why, the ability to distinguish between the various Aspergillus species may have diagnostic value.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of Molecular Variability Among Isolates of Aspergillus Flavus by PCR-RFLP of the its Regions of rDNA

Mohankumar¹,

Vijayasamundeeswari²,

Karthikeyan³

et al. 2010

Journal of Plant Protection Research

View full text Add to dashboard Cite

A total of seventeen isolates of Aspergillus flavus from maize were collected from different agro-ecological zones of Tamil Nadu, India. The isolates were tested for their ability to produce aflatoxin B 1 (AFB 1 ) in vitro by indirect competitive enzyme-linked immunosorbent assay (ELISA). The amount of AFB 1 produced by the isolates of A. flavus ranged from 1.9 to 206.6 ng/ml. Among the various isolates of A. flavus, the isolate AFM46 produced the highest amount of AFB 1 . DNA was extracted from A. flavus isolates and their molecular variability was investigated by using restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified internal transcribed spacer (ITS) regions of ribosomal DNA. PCR amplification with ITS1 and ITS4 primers resulted in the amplification of a product of approximately 600 bp. Digestion of the PCR products with the restriction enzymes EcoRI, HaeIII and TaqI produced fragments of different sizes. Analysis of the genetic coefficient matrix derived from the scores of RFLP profiles showed that minimum and maximum per cent similarities among the tested A. flavus strains ranged from 0 to 88%. Cluster analysis using the unweighted pair-group method with arithmetic average (UPGMA) clearly separated the isolates into five groups (group I-V) confirming the genetic diversity among the A. flavus isolates from maize.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Isolation Of Aspergillus Sppmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Molecular Variability Among Isolates of Aspergillus Flavus by PCR-RFLP of the its Regions of rDNA

Mohankumar¹,

Vijayasamundeeswari²,

Karthikeyan³

et al. 2010

Journal of Plant Protection Research

View full text Add to dashboard Cite

show abstract

“…Understanding such variation may provide clues as to the source of inoculum and new ideas for the control of A. flavus infection and aflatoxin contamination (Karthikeyan et al 2009). Based on aflatoxin production and size and number of sclerotia produced in vitro, A. flavus isolates can be divided into two groups, S and L (for small and large sclerotia, respectively; Cotty 1989).…”

Section: Phylogeography Of Aspergillus Flavusmentioning

confidence: 99%

Phylogeography of the cosmopolitan fungus Aspergillus flavus: is everything everywhere?

et al. 2012

View full text Add to dashboard Cite

“…A significant positive correlation between moisture content of maize seeds and A. flavus population and aflatoxin production has been reported (Oyebanji and Efiuvwevwere 1999). Karthikeyan et al (2009) …”

Section: Resultsmentioning

confidence: 99%

Prevalence of Aflatoxin B1 Contamination in Pre- and Post-Harvest Maize Kernels, Food Products, Poultry and Livestock Feeds in Tamil Nadu, India

Vijayasamundeeswari¹,

Mohankumar²,

Karthikeyan³

et al. 2009

Journal of Plant Protection Research

View full text Add to dashboard Cite

Aflatoxins, a group of mycotoxins mainly produced by Aspergillus flavus and A. parasiticus, have adverse health effects on humans and livestock that ingest aflatoxin-contaminated food products and feeds. To secure the safety of food and feed, regular monitoring of aflatoxin levels is necessary. In order to understand the magnitude of aflatoxin contamination, a survey was conducted in different agro-ecological zones of Tamil Nadu, India and 242 samples consisting of pre-and post-harvest maize kernels, food products, poultry and livestock feeds were collected from farmers' fields, poultry farms, retail shops and supermarkets and analyzed for aflatoxin B 1 (AFB 1 ) contamination by enzyme-linked immunosorbent assay (ELISA) using antiserum raised against aflatoxin B 1 -Bovine serum albumin (AFB 1 -BSA). The results indicated that 61.3% of the maize kernel samples were contaminated with AFB 1 and the levels of AFB 1 in 26% of the pre-and post-harvest maize kernels exceeded 20 µg/kg. The highest level of AFB 1 (245 µg/kg) was recorded in post-harvest maize kernel samples. In food products AFB 1 was detected only in two samples out of 30 samples tested.Furthermore, the levels ranged from 0.6 to 3.7 µg/kg. In poultry feeds, AFB 1 was detected in 30 out of 53 samples and the levels ranged from 0.7 to 31.6 µg/kg. Among the 40 livestock feed samples evaluated 29 samples were contaminated with AFB 1 at level ranging from 1.8 to 244.9 µg/kg.

show abstract

Genetic variability and aflatoxigenic potential ofAspergillus flavusisolates from maize

Cited by 13 publications

References 20 publications

Analysis of Molecular Variability Among Isolates of Aspergillus Flavus by PCR-RFLP of the its Regions of rDNA

Analysis of Molecular Variability Among Isolates of Aspergillus Flavus by PCR-RFLP of the its Regions of rDNA

Phylogeography of the cosmopolitan fungus Aspergillus flavus: is everything everywhere?

Prevalence of Aflatoxin B1 Contamination in Pre- and Post-Harvest Maize Kernels, Food Products, Poultry and Livestock Feeds in Tamil Nadu, India

Contact Info

Product

Resources

About