Genetic variability in two captive colonies of <i>Cebus apella paraguayanus</i> (primates: platyrrhini) from eastern Paraguay

Martinez, Romari Alejandra; Torres, Estela; Nieves, Mariela; Szapkievich, Valeria; Rodríguez, S; Schinini, Alicia; Ascurra, Marta; Mudry, Marta Dolores

doi:10.1080/00087114.2004.10589414

Cited by 12 publications

(6 citation statements)

References 29 publications

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“…A particular case is Cebus, which presents heteromorphisms of heterochromatic blocks throughout populations (Dutrillaux et al 1978;Seuánez et al 1986;Mudry de Pargament and Labal de Vinuesa 1988;Ponsà et al 1995;Martinez et al 2004;Nieves et al 2008).…”

Section: Introductionmentioning

confidence: 98%

Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons

Nieves

Oliveira²,

Amaral³

et al. 2011

J Genet

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The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), considered the most ancestral one, shows the greatest amount of heterochromatin described among Platyrrhini genera. Banding techniques and restriction enzyme digestion have previously revealed great variability of quantity and composition of heterochromatin in this genus. In this context, we use fluorescence in situ hybridization (FISH) to analyse this genomic region and discuss its possible role in the diversification of Cebus.We used a heterochromatin probe for chromosome 11 of Cebus libidinosus (11qHe+ CLI probe), obtained by chromosome microdissection. Twenty-six specimens belonging to the families Atelidae, Cebidae, Callitrichidae and Pithecidae (Platyrrhini) were studied. Fourteen out of 26 specimens were Cebus (Cebidae) individuals of C. libidinosus, C. xanthosternos, C. apella, C. nigritus, C. albifrons, C. kaapori and C. olivaceus. In Cebus specimens, we found 6 to 22 positive signals located in interstitial and telomeric positions along the different species. No hybridization signal was observed among the remaining Ceboidea species, thus reinforcing the idea of a Cebus-specific heterochromatin composed of a complex system of repetitive sequences.

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Section: Introductionmentioning

confidence: 98%

Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons

Nieves

Oliveira²,

Amaral³

et al. 2011

J Genet

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“…Our results are part of our research project on the genetics of primates in captivity in Argentinean institutions (Primates Project; Martinez et al 2004;Nieves et al 2003Nieves et al , 2005Nieves et al , 2007. In particular, we are the first to gather information from morphological, cytogenetic, and nucleotide variation within Saimiri boliviensis.…”

Section: Cytogenetic and Molecular Parametersmentioning

confidence: 99%

Morphological and Genetic Characterization of Saimiri boliviensis

et al. 2009

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The taxonomy of Saimiri is controversial because morphological characteristics, traditionally used for identification, are insufficient to distinguish species and subspecies. Genetic studies of specimens become relevant for captive management, especially considering their frequently unknown geographical origin. We analyzed phenotypic and genetic parameters in Saimiri spp. in Argentinean zoological gardens and biological stations to provide a more accurate taxonomic identification. We studied 27 males and 19 females of Saimiri spp. The cytogenetic analysis in mitotic metaphases corroborated a modal number of 2N=44, XX/XY, and FN=75 for males and FN=76 for females. G-and C-bands, fluorescence in situ hybridization (FISH) and the pelage coloration pattern of all the specimens corresponded to Saimiri boliviensis boliviensis. We characterized for the first time the sperm cell morphology and morphometry (mean±SE): total length: 71.39± 5.40 μm; head length: 5.71±0.81 μm; head width: 3.76±0.70 μm; acrosome length: 3.70±0.82 μm; midpiece length: 12.20±2.22 μm. Researchers can use the characterization of the sperm morphology as another parameter for taxonomic identification that, together with cytogenetic and molecular ones, would allow a more precise identification of individual Saimiri boliviensis boliviensis.

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“…Nieves et al (2017) reviewed the observations related to polymorphic variants that support the hypothesis of highly polymorphic chromosomal pairs, especially for S. nigritus and S. cay from the southernmost distribution. The polymorphisms observed in pair 13 in the individuals from Ilha da Marambaia probably resulted from paracentric inversion, the most common intraspecific rearrangement in Sapajus (Matayoshi et al 1987;Ponsà et al 1995;Garcia et al 2002;Martinez et al 2004;Ruiz-Herrera et al 2004), besides duplication.…”

Section: Discussionmentioning

confidence: 95%

Genetic analysis of an insular population of Sapajus nigritus (Primates: Cebidae) in Rio de Janeiro state, Brazil

et al. 2021

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The black-horned capuchin (Sapajus nigritus) is a neotropical primate with wide distribution from southeastern Brazil to northeastern Argentina. Although this species has been described with coat pattern variation, even with intrapopulational differences, and characterized as having the greatest genetic diversity among Sapajus species, there are still few studies on natural populations that contribute to the knowledge of this intraspecific variability. We examined individuals from an as yet unstudied population of Ilha da Marambaia, Rio de Janeiro (RJ) state, Brazil, compared with published data for S. nigritus. We sought to confirm the species through phenotypic and genetic characterization using C-banding and fluorescence in situ hybridization with #11qHe+/21WCP probes for chromosomal constitutive heterochromatin (He+) patterns, and cytochrome c oxidase I and II gene sequences for phylogenetic analysis. The coat presented two color patterns, varying from brown to blackish on the body, yellow to brown on the chest, and white to yellow on the face, besides the presence and shape of the tufts on the head, corresponding to S. nigritus. He+ was identified in pairs 4, 12, 13 and 17, and less consistently in pairs 6, 19 and 21, already described for this species. While most Sapajus species have a large He+ block, here pair 11 was identified without extracentromeric He+, the same as reported for S. nigritus from Argentina. Molecular analysis showed divergence of this population from other S. nigritus sequences, reinforcing a trend already demonstrated when samples from RJ are compared with the rest of the distribution, which may represent an evolutionary deviation.

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Genetic variability in two captive colonies of Cebus apella paraguayanus (primates: platyrrhini) from eastern Paraguay

Cited by 12 publications

References 29 publications

Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons

Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons

Morphological and Genetic Characterization of Saimiri boliviensis

Genetic analysis of an insular population of Sapajus nigritus (Primates: Cebidae) in Rio de Janeiro state, Brazil

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