Genetic variability of Citrus spp. in Cibinong germplasm garden using random amplified polymorphic DNA

Abstract

The preservation and protection of citrus plant germplasms can be done in-situ and ex-situ. Germplasm garden LIPI, Cibinong was built as a local fruit plant garden. The field management also carries out seed propagation and dissemination. This study aims to reveal the genetic diversity of citrus collections and used them as a database for plant breeding and protection activities. This research was conducted at the Agronomy Laboratory for Evaluation of Biotechnology Products-LIPI. Genetic analysis was performed using RAPD techniques, and the resulting data were processed using PowerMarkerver 3.25 and PASTver 3.25 programs. The results showed that seven genetic materials for citrus analyzed with 15 RAPD primers resulted in the mean number of alleles, frequency of main alleles, and heterozygosity are 7.33, 0.49, and 1, respectively. All primers had a PIC value > 0.5, meaning they had high information in fingerprinting research on citrus plant genetic diversity, with an average gene diversity of 0.71. Principal coordinates analysis [PCoA] and clustering obtained two groups with a 0.70 similarity coefficient. Garut and Siam Pontianak have the closest relationship [92% similarity]. On the other hand, the farthest genetic relationship is between Bali and Keprok Batu-55, with a similarity index value of 52%.

Short Communication: Analysis of detected metabolites compounds from the crude extract of Rimau Gerga Lebong oranges fruit (Citrus reticulata ‘RGL’) using LC-QTOF-MS/MS

“…It indicated that the bioactive compounds of C. reticulata from different planting locations might differ from the same species (Abeysinghe et al 2007;Wang et al 2019;Wang et al 2020). The difference in secondary metabolites could be due to genetic variations, availability of nutrients, and environmental conditions where they are grown (Anggraheni 2021;Siagian et al 2021). In addition, the difference in the number of identified compounds may be influenced by the sensitivity of the LC-QTOF-MS/MS used.…”

Application of RAPD Molecular Technique to Study the Genetic Variations of Citrus in South Sulawesi, Indonesia

“…One of Indonesia's leading horticultural commodities is fruit, and Indonesia has local superior citrus species and varieties that are spread throughout the archipelago. Citrus is one of the world's major fruit crops [3], grown in many regions [4], which has high economic value [5]. Citrus fruits belong to the family Rutaceae and subfamily Aurantioideae and can be grown in tropical and sub-tropical climates [6].…”