Genetic variation at the alpha‐1‐fucosyltransferase (<i>FUT1</i>) gene in Asian wild boar and Chinese and Western commercial pig breeds

Bao, Wenbin; Wu, Sen; Musa, Hassan Hussein; Zhu, Guoqiang; Chen, G.H.

doi:10.1111/j.1439-0388.2008.00722.x

Cited by 49 publications

(40 citation statements)

References 4 publications

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“…It is clear that FUT1 and FUT2 loci are associated with E. coli F18 adhesion resistant animals in these breeds. The research confirmed that a variation in the alpha 1 fucosyltransferase (FUT1) gene is the causative mutation for adhesion-resistant animals (Michaels et al, 1994;Bao et al, 2008). In addition to the F18-resistance marker, a marker for E. coli K88 has been identified (Michaels et al, 1994).…”

Section: Introductionmentioning

confidence: 57%

Polymorphisms of candidate genes associated with meat quality and disease resistance in indigenous and exotic pig breeds of Vietnam

Cuong¹,

Thu²,

Thoa³

et al. 2012

SA J. An. Sci.

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The objectives of this study were to analyse genotype distribution and sequence variations of candidate genes putatively associated with meat quality and disease resistance in exotic and indigenous Vietnamese pig breeds. For this purpose, 340 pigs from four indigenous and two exotic breeds were included in the analysis of the polymorphisms of the heart fatty-acid-binding protein (H-FABP), alpha 1 fucosyltransferase (FUT1), and bactericidal/permeability-increasing protein (BPI) genes by the sequencing and PCR-RFLP methods. For H-FABP, 17 single nucleotide polymorphisms (SNPs) were detected in indigenous pig breeds by direct sequencing of a fragment at intron 2 of the H-FABP gene. The mutation T1556C created a new restriction site for the enzyme MspI, which gave rise to new allelic variants in three indigenous pig breeds. In indigenous breeds, the frequency of the favourable alleles a and d at MspI and HaeIII sites of the H-FABP gene were low. Meanwhile, the frequency of the d allele at the HaeIII site in exotic breeds was significantly higher than those of indigenous pig breeds. No mutation was found in the RFLP-fragment of the FUT1 gene of four indigenous pig breeds by sequencing, while in the BPI gene two mutations were detected in the Tap Na breed. The resistant alleles of the FUT1 and BPI genes in the exotic breeds were significantly higher than those of indigenous pig breeds. Among the indigenous pig breeds, the Tap Na breed possessed a higher frequency of the resistant allele G of BPI gene than the remaining breeds. The T1556C mutation at H-FABP may be important for the genetic improvement of intramuscular fat content and breed. Tap Na may be a source of resistant alleles for local ecologies.

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Section: Introductionmentioning

confidence: 57%

Polymorphisms of candidate genes associated with meat quality and disease resistance in indigenous and exotic pig breeds of Vietnam

Cuong¹,

Thu²,

Thoa³

et al. 2012

SA J. An. Sci.

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“…Meijerink et al (1997;2000) reported that a mutation in the M307 locus of FUT1 confers resistance to E. coli infections. However, the distributions of genotypes at this locus are highly skewed among Chinese porcine breeds; therefore, this gene is not suitable for breeding disease resistance (Bao et al, 2008). So screening and identifying the gene conferring resistance to E. coli F18 and search for novel genetic markers are very important.…”

Section: Introductionmentioning

confidence: 99%

Developmental expression of LTβR and differential expression in Escherichia coli F18 resistant/sensitive piglets

Sun

et al. 2016

Genet. Mol. Res.

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ABSTRACT. We analyzed LTβR mRNA expression in piglets from birth to weaning and compared the differential expression between Escherichia coli F18-resistant and sensitive populations to determine whether this gene could be used as a genetic marker for E. coli F18 resistance. Sutai piglets of different age groups (8, 18, 30, and 35 days; N = 4 each) and piglets demonstrating resistance/sensitivity to E. coli F18 were used. LTβR expression levels were determined by real-time PCR. The LTβR expression levels in the lymph node, duodenum, and jejunum were significantly higher in 8-day-old piglets than in the other age groups (P < 0.01), and the expression levels were significantly 2 R.W. Xia et al. Genetics and Molecular Research 15 (3): gmr.15038377 higher in the lungs of 8-day-old piglets than in 35-day-old piglets (P < 0.01) and 30 day-old piglets (P < 0.05). In liver tissue, the expression level was significantly higher in the 35-day-old piglets than in other age groups (P < 0.01). In the stomach tissue, the expression level was significantly higher in 35-day-old piglets than in 18-day-old piglets (P < 0.05). LTβR expression in the lymph nodes was significantly higher in the resistant group than in the sensitive group (P < 0.01), but there was no significant difference in the other tissues (P > 0.05). These results indicate that 8 days after birth is a crucial stage in the formation of mesentery lymph nodes and immune barriers in pigs, and increased expression of LTβR may be beneficial for developing resistance to E. coli F18.

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“…Based on this research, we and other Chinese researchers have systematically analyzed the FUT1-coding region in nearly 30 Chinese native pig breeds and foreign pig breeds. The variation of FUT1 gene on M307 locus, which has been used as a genetic marker in foreign breeds, displays an extremely skewed distribution in native Chinese pig breeds compared to foreign breeds (Bao et al 2008;Yan et al 2003), and the presence or absence of the M307 locus does not significantly influence the expression level of FUT1 (Bao et al 2012a, b). This makes the development of resistance to ETEC F18 in local Chinese pig breeds difficult and problematic.…”

Section: Introductionmentioning

confidence: 99%

Correlation between the methylation of the FUT1 promoter region and FUT1 expression in the duodenum of piglets from newborn to weaning

et al. 2017

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Alpha-(1,2)-fucosyltransferase (FUT1) gene has some influence on economically important traits and disease resistance. DNA methylation plays an important role in human diseases but is relatively poorly studied in pigs by regulating the mRNA expression of genes. The aim of this study was to analyze the influence of promoter methylation on the expression of FUT1 gene. We used bisulfite sequencing PCR (BSP) and qPCR to analyze the methylation of the FUT1 5 0 -flanking region and FUT1 mRNA expression in the duodenum of Sutai piglets from newborn to weaning. FUT1 contains three CpG islands upstream of the start codon, of which two are located in the putative promoter region containing multiple promoter elements and transcription factor binding sites, such as CpG islands, a CAAT box, SP1, and EARLY-SEQ 1. The CpG island between nucleotides -1762 and -580 had a low degree of methylation, and its methylation level was significantly lower in 35-day-old piglets than 8-and 18-day-old piglets (P \ 0.05). FUT1 mRNA expression was significantly higher in 35-day-old piglets than 8-and 18-day-old piglets (P \ 0.05). Pearson's correlation analysis showed that the methylation of the CpG island between nucleotides -1762 and -580 of FUT1 was significantly, negatively correlated with FUT1 mRNA expression (P \ 0.05). These results demonstrate that differential methylation of CpG islands negatively regulates the expression of FUT1 in the porcine duodenum, suggesting a probable influence on the resistance of piglets to infection with ETEC F18.

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Genetic variation at the alpha‐1‐fucosyltransferase (FUT1) gene in Asian wild boar and Chinese and Western commercial pig breeds

Cited by 49 publications

References 4 publications

Polymorphisms of candidate genes associated with meat quality and disease resistance in indigenous and exotic pig breeds of Vietnam

Polymorphisms of candidate genes associated with meat quality and disease resistance in indigenous and exotic pig breeds of Vietnam

Developmental expression of LTβR and differential expression in Escherichia coli F18 resistant/sensitive piglets

Correlation between the methylation of the FUT1 promoter region and FUT1 expression in the duodenum of piglets from newborn to weaning

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