Genetic variation of human respiratory syncytial virus among children with fever and respiratory symptoms in Shanghai, China, from 2009 to 2012

Liu, Jia; Mu, Yonglin; Dong, Wei; Yao, Fujia; Wang, Lili; Yan, Huajie; Lan, Ke; Zhang, Chiyu

doi:10.1016/j.meegid.2014.07.011

Cited by 28 publications

(45 citation statements)

References 17 publications

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“…In our study, the ON1 genotype was first detected in Shanghai in February 2011, which was earlier than its detection in Beijing in 2012 as reported previously 15, 29 . After that, the ON1 genotype became more and more prevalent, and replaced the NA1 genotype to become the predominant genotype in China between 2013 and 2015.…”

Section: Discussionsupporting

confidence: 69%

“…According to the genetic characteristic and reactivity with monoclonal antibodies, HRSV is classified into 2 subgroups, A (HRSVA) and B (HRSVB) 11 . To date, there are 15 genotypes of HRSVA (GA1-GA7, SAA1, CB-A, NA1-4 and ON1-2) 12–15 , and 24 genotypes of HRSVB (GB1-GB4, SAB1-SAB4, URU1-2, BA1-12, GB5/CB1 and CBB) 16–20 . The HRSVA genotype ON1 was first detected with a 72-nucleotide duplication in the HVR2 region encoded by the G gene in Canada in 2010.…”

Section: Introductionmentioning

confidence: 99%

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Emergence of ON1 genotype of human respiratory syncytial virus subgroup A in China between 2011 and 2015

Song

Zhang

Wang

et al. 2017

Sci Rep

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A molecular epidemiological study of human respiratory syncytial virus (HRSV) was conducted to examine the distribution of its subgroups and genotypes, as well as to identify its transmission pattern in China. A total of 705 samples collected from 9 provinces in China between January 2008 and February 2015 were identified as HRSV-positive and were subsequently sequenced. Of these, 336 samples were HRSV subgroup A (HRSVA), 368 samples were HRSV subgroup B (HRSVB), and 1 sample contained both HRSVA and HRSVB. These 705 HRSV sequences, together with 766 HRSV sequences downloaded from GenBank, were analyzed to understand the recent circulation patterns of HRSV in China. HRSVB predominated in the 2008/2009 and 2009/2010 seasons, whereas HRSVA predominated in the 2010/2011 and 2011/2012 seasons; HRSVA and HRSVB co-circulated during 2012/2013 and 2014/2015. Phylogenetic analysis showed most of the HRSVA sequences clustered into 2 genotypes, namely, NA1 and ON1. The ON1 genotype was first detected in China in 2011, and it quickly replaced the NA1 genotype to become the most prevalent HRSVA genotype circulating in China between 2013 and 2015. Continuous epidemiological surveillance and molecular characterization of HRSV should be conducted to monitor the evolution of HRSV in China.

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Section: Discussionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Emergence of ON1 genotype of human respiratory syncytial virus subgroup A in China between 2011 and 2015

Song

Zhang

Wang

et al. 2017

Sci Rep

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“…HRSV strains with this 72 nucleotide duplication were designated genotype ON1. Up to date, HRSV genotype ON1 has been found in China (Cui et al, 2013b;Liu et al, 2014;Ren et al, 2014), Japan (Hirano et al, 2014;Tsukagoshi et al, 2013), South Korea (Lee et al, 2012), Germany (Prifert et al, 2013), South Africa (Valley-omar et al, 2013), Malaysia (Khor et al, 2013), Italy (Pierangeli et al, 2014), India (Choudhary et al, 2013), Thailand (Auksornkitti et al, 2013), Kenya (Agoti et al, 2014), Latvia (Balmaks et al, 2013), Spain (GenBank accession number: KF915266), Philippines (AB846656), Peru (KJ627264), Panama (KF301013), Croatia (KF057868) and USA (KJ672471).…”

Section: Introductionmentioning

confidence: 99%

Rapid replacement of prevailing genotype of human respiratory syncytial virus by genotype ON1 in Beijing, 2012–2014

Cui

Zhu

Zhao

et al. 2015

Infection, Genetics and Evolution

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“…To evaluate the performance of the novel assay, 40 nasopharyngeal swabs collected from children with ARTIs in our previous studies were used [29,30]. Among these samples, half were previously detected as RSV-positive and half as RSV-negative using our in-house multiplex RT-qPCR method.…”

Section: Methodsmentioning

confidence: 99%

A Mismatch-Tolerant RT-Quantitative PCR: Application to Broad-Spectrum Detection of Respiratory Syncytial Virus

Wan

et al. 2019

BioTechniques

Self Cite

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Quantitative PCR (qPCR) is widely used to detect viruses. However, mismatches occurring in the 3′-end of the primers reduce amplification efficiency of qPCR and limit its capacity in detection of highly variable viruses. Here, we reported a mismatch-tolerant RT-qPCR with a small amount of additional high-fidelity DNA polymerase for simultaneous detection of RSV-A and RSV-B. The novel assay had higher amplification efficiency for various variants forming mismatches with the primers than the conventional RT-qPCR, and showed good specificity and sensitivity. It demonstrated a good correlation coefficient with a commercial RSV detection kit and had relatively lower Ct values than the kit for 16 of 20 RSV-positive samples. The mismatch-tolerant qPCR technique is a promising approach for sensitive detection of highly variable viruses.

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Genetic variation of human respiratory syncytial virus among children with fever and respiratory symptoms in Shanghai, China, from 2009 to 2012

Cited by 28 publications

References 17 publications

Emergence of ON1 genotype of human respiratory syncytial virus subgroup A in China between 2011 and 2015

Emergence of ON1 genotype of human respiratory syncytial virus subgroup A in China between 2011 and 2015

Rapid replacement of prevailing genotype of human respiratory syncytial virus by genotype ON1 in Beijing, 2012–2014

A Mismatch-Tolerant RT-Quantitative PCR: Application to Broad-Spectrum Detection of Respiratory Syncytial Virus

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