Genistein alleviates H
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            O
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            -induced senescence of human umbilical vein endothelial cells via regulating the TXNIP/NLRP3 axis

Wu, Guihua; Li, Siming; Qu, Guangjin; Hua, Jiajia; Zong, Jing; Li, Xiaofeng; Xu, Fanghui

doi:10.1080/13880209.2021.1979052

Cited by 18 publications

(8 citation statements)

References 42 publications

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“…Finally, targeted drugs for the treatment of senescence-related AMI were also explored in this study. N-acetylcysteine and genistein have been reported to play a protective role in cellular senescence ( 53 , 54 ). Bisphenol A, bleomycin, cisplatin and dexamethasone have been reported to induce cell senescence under some special circumstances ( 55 – 58 ).…”

Section: Discussionmentioning

confidence: 99%

Identification and validation of senescence-related genes in circulating endothelial cells of patients with acute myocardial infarction

Xiang

Shen

Zhang

et al. 2022

Front. Cardiovasc. Med.

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BackgroundAcute myocardial infarction (AMI) is the main clinical cause of death and cardiovascular disease and thus has high rates of morbidity and mortality. The increase in cardiovascular disease with aging is partly the result of vascular endothelial cell senescence and associated vascular dysfunction. This study was performed to identify potential key cellular senescence-related genes (SRGs) as biomarkers for the diagnosis of AMI using bioinformatics.MethodsUsing the CellAge database, we identified cellular SRGs. GSE66360 and GSE48060 for AMI patients and healthy controls and GSE19322 for mice were downloaded from the Gene Expression Omnibus (GEO) database. The GSE66360 dataset was divided into a training set and a validation set. The GSE48060 dataset was used as another validation set. The GSE19322 dataset was used to explore the evolution of the screened diagnostic markers in the dynamic process of AMI. Differentially expressed genes (DEGs) of AMI were identified from the GSE66360 training set. Differentially expressed senescence-related genes (DESRGs) selected from SRGs and DEGs were analyzed using Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and protein-protein interaction (PPI) networks. Hub genes in DESRGs were selected based on degree, and diagnostic genes were further screened by gene expression and receiver operating characteristic (ROC) curve. Finally, a miRNA-gene network of diagnostic genes was constructed and targeted drug prediction was performed.ResultsA total of 520 DEGs were screened from the GSE66360 training set, and 279 SRGs were identified from the CellAge database. The overlapping DEGs and SRGs constituted 14 DESRGs, including 4 senescence suppressor genes and 10 senescence inducible genes. The top 10 hub genes, including FOS, MMP9, CEBPB, CDKN1A, CXCL1, ETS2, BCL6, SGK1, ZFP36, and IGFBP3, were screened. Furthermore, three diagnostic genes were identified: MMP9, ETS2, and BCL6. The ROC analysis showed that the respective area under the curves (AUCs) of MMP9, ETS2, and BCL6 were 0.786, 0.848, and 0.852 in the GSE66360 validation set and 0.708, 0.791, and 0.727 in the GSE48060 dataset. In the GSE19322 dataset, MMP9 (AUC, 0.888) and ETS2 (AUC, 0.929) had very high diagnostic values in the early stage of AMI. Finally, based on these three diagnostic genes, we found that drugs such as acetylcysteine and genistein may be targeted for the treatment of age-related AMI.ConclusionThe results of this study suggest that cellular SRGs might play an important role in AMI. MMP9, ETS2, and BCL6 have potential as specific biomarkers for the early diagnosis of AMI.

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Section: Discussionmentioning

confidence: 99%

Identification and validation of senescence-related genes in circulating endothelial cells of patients with acute myocardial infarction

Xiang

Shen

Zhang

et al. 2022

Front. Cardiovasc. Med.

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“…Lots of researches have suggest that traditional Chinese medicine has good effects on cardiovascular aging, which plays an anti-cardiovascular aging role through mechanism such as anti-oxidation, inhibition of apoptosis, anti-inflammatory, and improving immunity, thereby preventing and treating cardiovascular diseases [21][22][23][24]. Hua et al believes that traditional Chinese medicine can significantly improve the percentage of Tlymphocyte subsets and the activity of NK cells, thereby improving the immune function and disease resistance of the elderly [25].…”

Section: Discussionmentioning

confidence: 99%

Whole-transcriptome sequencing analysis reveal mechanisms of Yiqi Huoxue Yangyin (YHY) decoction in ameliorating D-gal-induced cardiac aging

Wang

Zhang

Xiu

et al. 2023

Aging

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Background: Aging is a major factor for cardiovascular disease, and cardiac aging is closely related to the incidence of cardiovascular disease. Clarifying the mechanism of cardiac aging and finding reliable intervention is critical for preventing cardiovascular diseases and achieving healthy longevity. Traditional Chinese medicine Yiqi Huoxue Yangyin (YHY) decoction has unique advantage in the treatment of cardiovascular disease and aging. However, the associated molecular mechanisms remain unknown. Purpose: The present study aimed to verify the efficacy of YHY decoction against cardiac aging in D-gal-induced mouse model, and explore the potential mechanism of YHY decoction treatment through whole-transcriptome sequencing technique, providing novel insights into the molecular basis of YHY decoction in treating cardiac aging. Methods: The component of YHY decoction was identified by High Performance Liquid Chromatography (HPLC). Dgal-induced aging mouse model was established for this study. HE and Masson staining were applied to determine pathological changes of heart; telomere length, telomerase activity, AGEs and p53 were used to evaluate the degree of heart aging. Transcriptome sequencing, GO, KEGG, GSEA and ceRNA network were applied to analyze the potential mechanism of YHY decoction treatment of cardiac aging.Results: In this study, we found that YHY decoction not only improved the pathological structure of aging heart, but also regulated the expression of aging-related markers, telomere length, telomerase activity, AGEs and p53, the myocardial tissue, suggesting that it has a specific effect in delaying cardiac aging. Whole-transcriptome sequencing showed that the total of 433 mRNAs, 284 lncRNAs, 62 miRNAs, and 39 circRNAs were significantly differentially expressed after YHY decoction treatment. According to the analysis results of KEGG and GSEA, the differentially expressed mRNAs were found significantly involved in immune system, cytokine-cytokine receptor interaction and cell adhesion molecules. The ceRNA network showed that miR-770, miR-324, and miR-365 are localized in center, mainly affecting the immune system, PI3K-Akt signaling pathway, and MAPK signaling pathway. Conclusion: In conclusion, our results evaluated the ceRNA network of YHY decoction in treating cardiac aging for the first time, which could provide better understanding of the potential mechanism of YHY decoction treatment of cardiac aging.

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“…Lots of research have suggest that traditional Chinese medicine has good effects on cardiovascular aging, which plays an anti-cardiovascular aging effect through mechanisms such as anti-oxidation, inhibition of apoptosis, anti-in ammatory, and improving immunity, thereby preventing and treating cardiovascular diseases [21][22][23][24]. Hua et al believes that traditional Chinese medicine can signi cantly improve the percentage of T-lymphocyte subsets and the activity of NK cells, thereby improving the immune function and disease resistance of the elderly [25].…”

Section: Discussionmentioning

confidence: 99%

Whole-Transcriptome Sequencing Analysis Reveal Mechanisms of Yiqi Huoxue Yangyin (YHY) Decoction in Ameliorating D-gal-induced Cardiac Aging

Wang

Xiu

Yang

et al. 2022

Preprint

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Background Aging is a major factor for cardiovascular disease, and cardiac aging is closely related to the incidence of cardiovascular disease. Clarifying the mechanism of cardiac aging and finding reliable intervention is critical for preventing cardiovascular diseases and achieving healthy longevity. Traditional Chinese medicine Yiqi Huoxue Yangyin (YHY) decoction has unique advantages in the treatment of cardiovascular disease and aging. However, the associated molecular mechanisms remain unknown. Purpose The present study aimed to verify the efficacy of YHY decoction against cardiac aging in D-gal-induced mouse model, and explore the potential mechanisms of YHY decoction treatment through whole-transcriptome sequencing technique, providing novel insights into the molecular basis of YHY decoction in treating cardiac aging. Methods The component of YHY decoction was identified by High Performance Liquid Chromatography (HPLC). D-gal-induced aging mouse model was established for this study. HE and Masson staining were applied to determined pathological changes of heart; telomere length, telomerase activity, AGEs and p53 were used to evaluated the degree of heart aging. Transcriptome sequencing, GO, KEGG, GSEA and ceRNA network were applied to analyzing the potential mechanisms of YHY decoction treatment of cardiac aging. Results In this study, we found that YHY decoction not only improved the pathological structure of aging heart, but also regulated the expression of aging-related markers, telomere length, telomerase activity, AGEs and p53, in the myocardial tissue, suggesting that it has a specific effect in delaying cardiac aging. Whole-transcriptome sequencing showed that the total of 433 mRNAs, 284 lncRNAs, 62 miRNAs, and 39 circRNAs were significantly differentially expressed after YHY decoction treatment. According to the analysis results of KEGG and GSEA, the differentially expressed mRNAs were found significantly involved in immune system, cytokine-cytokine receptor interaction and cell adhesion molecules. The ceRNA network showed that miR-770, miR-324, and miR-365 are localized in the center, mainly affecting the immune system, PI3K-Akt signaling pathway, and MAPK signaling pathway. Conclusion In conclusion, our results evaluated the ceRNA network of YHY decoction in treating cardiac aging for the first time, which could provide a better understanding of the potential mechanisms of YHY decoction treatment of cardiac aging.

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Genistein alleviates H ₂ O ₂ -induced senescence of human umbilical vein endothelial cells via regulating the TXNIP/NLRP3 axis

Cited by 18 publications

References 42 publications

Identification and validation of senescence-related genes in circulating endothelial cells of patients with acute myocardial infarction

Identification and validation of senescence-related genes in circulating endothelial cells of patients with acute myocardial infarction

Whole-transcriptome sequencing analysis reveal mechanisms of Yiqi Huoxue Yangyin (YHY) decoction in ameliorating D-gal-induced cardiac aging

Whole-Transcriptome Sequencing Analysis Reveal Mechanisms of Yiqi Huoxue Yangyin (YHY) Decoction in Ameliorating D-gal-induced Cardiac Aging

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Genistein alleviates H 2 O 2 -induced senescence of human umbilical vein endothelial cells via regulating the TXNIP/NLRP3 axis

Cited by 18 publications

References 42 publications

Identification and validation of senescence-related genes in circulating endothelial cells of patients with acute myocardial infarction

Identification and validation of senescence-related genes in circulating endothelial cells of patients with acute myocardial infarction

Whole-transcriptome sequencing analysis reveal mechanisms of Yiqi Huoxue Yangyin (YHY) decoction in ameliorating D-gal-induced cardiac aging

Whole-Transcriptome Sequencing Analysis Reveal Mechanisms of Yiqi Huoxue Yangyin (YHY) Decoction in Ameliorating D-gal-induced Cardiac Aging

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Genistein alleviates H ₂ O ₂ -induced senescence of human umbilical vein endothelial cells via regulating the TXNIP/NLRP3 axis