Genome activation and developmental block in bovine embryos

Meirelles, Flávio Vieira; Caetano, Alexandre Rodrigues; Watanabe, Yeda Fumie; Ripamonte, Paula; Carambula, Silvia; Merighe, Giovana Krempel Fonseca; Garcia, Sheila Merlo

doi:10.1016/j.anireprosci.2004.05.012

Cited by 116 publications

(106 citation statements)

References 30 publications

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“…In Vitro maturation is a method in which immature oocytes are retrieved from the ovary and are allowed to mature in the laboratory which is a crucial step for fertilization and subsequent embryonic development (Meirelles et al, 2004 andDadarwal et al, 2015). However, IVM of buffalo oocytes obtained poor recovery of good quality immature oocytes (Palta and Chauhan, 1998).…”

Section: Issn: 2319-7706 Volume 7 Number 04 (2018)mentioning

confidence: 99%

Effect of Ovarian Status on Oocyte Quality and Recovery Rate Retrieved by Aspiration Method in Buffalo Ovaries

Rajesh¹,

Swathi²,

Kumari³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Section: Issn: 2319-7706 Volume 7 Number 04 (2018)mentioning

confidence: 99%

Effect of Ovarian Status on Oocyte Quality and Recovery Rate Retrieved by Aspiration Method in Buffalo Ovaries

Rajesh¹,

Swathi²,

Kumari³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…The high rates of embryo mortality might be due to developmental block, a phenomenon described in many species that occurs concomitantly with major genome activation (Meirelles et al, 2004). Embryonic arrest during major genome activation occurs in cattle during the transition from the 4th to the 5th cell cycle due to the inability to modify the repressive chromatin structure and to activate the transcription of genes that are important for development (Betts & King, 2001).…”

Section: Introductionmentioning

confidence: 99%

Supplementation with the histone deacetylase inhibitor trichostatin A during in vitro culture of bovine embryos

Oliveira

Saraiva

Souza

et al. 2011

Zygote

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SummaryTrichostatin A (TSA) is a histone deacetylase inhibitor that induces histone hyperacetylation and increases gene expression levels. The aim of the present study was to establish a suitable condition for the use of TSA in in vitro cultures of bovine embryos, and to determine whether TSA would increase blastocyst rates by improvement of chromatin remodelling during embryonic genome activation and by increasing the expression of crucial genes during early development. To test this hypothesis, 8-cell embryos were exposed to four concentrations of TSA for different periods of time to establish adequate protocols. In a second experiment, three experimental groups were selected for the evaluation of embryo quality based on the following parameters: apoptosis, total cell number and blastocyst hatching. TSA promoted embryonic arrest and degeneration at concentrations of 15, 25 and 50 nM. All treated groups presented lower blastocyst rates. Exposure of embryos to 5 nM for 144 h and to 15 nM for 48 h decreased blastocyst hatching. However, the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (TUNEL) assay revealed similar apoptosis rates and total cell numbers in all groups studied. Although, in the present study, TSA treatment did not improve the parameters studied, the results provided background information on TSA supplementation during in vitro culture of bovine embryos and showed that embryo quality was apparently not affected, despite a decrease in blastocyst rate after exposure to TSA.

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“…O potencial de desenvolvimento até o estádio de blastocisto está associado a características de qualidade inerentes ao oócito e à interação deste embrião com meios e ambientes de cultivo (BREVINI et al, 2002;GANDOLFI, 2001;GUTIÉRREZ-ADAN et al, 2004;HYTTEL et al, 2001;LONERGAN et al, 2003;MEIRELLES et al, 2004;MOUROT et al, 2006;WRENZYCKI, 2000;RIZOS et al, 2002).…”

Section: Introductionunclassified

“…Assim, o destino de uma célula seria determinado pela habilidade dos supressores da MCP em seqüestrarem ou neutralizarem a ação dos indutores do processo (BADR et al, 2007;JURISICOVA et al,1998;MEIRELLES et al, 2004;OPIELA et al, 2008;RAJAMAHENDRAN, 2002).…”

Section: Introductionunclassified

“…Realmente, foi detectada uma maior proporção de transcritos em embriões com alta taxa de bloqueio do que naqueles embriões ditos competentes ao desenvolvimento (DODE et al, 2006;FAVETTA et al, 2007;FIGUEIREDO, 2005;JEONG et al, 2006;MATWEE;KING, 2000;MEIRELLES et al, 2004). Estudos observaram a ocorrência de MCP de forma espontânea apenas após a AGE, ou que com o uso de indutores químicos, a apoptose ocorreu em maior grau após a AGE, contrariando que a MCP seria um processo passivo por ausência de transcrição GJORRET et al, 2007).…”

Section: Introductionunclassified

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Ativação de genes apoptóticos no bloqueio do desenvolvimento em embriões bovinos

Cortezzi¹

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Ao Léo, que entre muitas idas e vindas ao frigorífico, trazia não só os ovários, mas também seu sorriso e alto astral todos os dias. À Giovana, sempre muito solícita em ajudar em todas as etapas, incluindo nesta fase final devido à minha ausência. Obrigada pelo auxílio com os documentos! 5 A todos que fazem ou já fizeram parte do LMMD. São tantas pessoas ao longo destes anos que seria injusto citar nomes. Obrigada a todos por me ensinar que diferenças entre as pessoas fazem parte da vida, auxiliar com aspirações de ovários, ensinar procedimentos, discutir idéias e problemas, dividir churrascos e festas, e também auxiliar à distância sempre que necessário! Em especial ao Marcos e ao Moysés, fundamentais em algumas discussões deste trabalho.Às amigas da FZEA Claudinha e Fran, pela amizade e companhia nas baladas pirassununguenses, e à Estelinha, pela amizade e estadia nos momentos finais.Ao pessoal da dança, Marcos Mendonça, Marcos, Ursiley e Silvana, pela amizade e descontração não só nas aulas, mas principalmente fora delas. Com vocês eu pude relaxar entre boleros, sambas, tangos, salsas, forrós... Mesmo agora que estamos cada um em uma cidade, vocês sempre serão minha "turma de dança"! Aos meus amigos de Jaboticabal, antigos ou adquiridos pós-Ronaldo, pelos poucos mas animados finais de semana na terra natal. Em especial à Nádia, Dani (estadias e apoio em Sampa), Raqs Lima, Raqs Costa, Clau Dorigan, Márcia e família.Aos meus amigos da fase de São José do Rio Preto. Entre os muitos, destaco a Lê Vidotto (e seus pais) e a Lú Bizari, sempre disponibilizando suas casas para minha estadia. À Cássia e seus pais pelo carinho e amizade. Ao Eduardão, ao Adriano, ao Alfredo e ao Amílcar, que ficam na torcida de longe, via internet, me dão os puxões de orelha necessários e fazem minha vida mais feliz.Ao pessoal da Associação Instituto Sapientiae -Clínica Fertility, por acreditarem na minha capacidade profissional. analyze gene expression differences between fast cleavage and slow cleavage embryos, and discover possible signaling pathways to cell death or cell survival. We used a macroarray membrane spotted with human genes related to cell cycle and hybridizated with a radioactive labeled aRNA from fast or slow in vitro produced embryos. Real-time PCR and signaling pathways analysis were designed for further validation of the array. The mean similarity between human and bovine genes was 89,3%. According to the array membranes, it was possible to identify 120 genes differentially expressed between slow and fast cleavage embryos. Hence, the majority of the genes were more expressed in slow embryos (100 genes versus 20 genes in fast group). Among genes more expressed at fast embryos, 40%were identified as protein binding and 25% have catalytic activity, with similar results in slow embryos. In one hand, differences between fast and slow embryos transcripts were not confirmed by real-time PCR analysis. But on the other hand, the differentially expressed genes are somehow related to and constitutively present in some recognized...

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Genome activation and developmental block in bovine embryos

Cited by 116 publications

References 30 publications

Effect of Ovarian Status on Oocyte Quality and Recovery Rate Retrieved by Aspiration Method in Buffalo Ovaries

Effect of Ovarian Status on Oocyte Quality and Recovery Rate Retrieved by Aspiration Method in Buffalo Ovaries

Supplementation with the histone deacetylase inhibitor trichostatin A during in vitro culture of bovine embryos

Ativação de genes apoptóticos no bloqueio do desenvolvimento em embriões bovinos

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