Genome complexities of the three mRNA species of snowshoe hare bunyavirus and in vitro translation of S mRNA to viral N polypeptide

Cash, Phillip; Vezza, Anne C.; Gentsch, Jon R.; Bishop, David H. L.

doi:10.1128/jvi.31.3.685-694.1979

Cited by 48 publications

(20 citation statements)

References 15 publications

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“…Virus, cells and preparation of infected cell RNA BHK-2 I cells were grown to confluence in Eagle's medium containing 10% newbom calf serum and infected with SSH virus at a multiplicity of 5 to 10. The cells were incubated for 10 hr at 370C in the presence of (3H)adenosine to label RNA and extracted for total cytoplasmic RNA as described previously (5,17). In vitro translation of mRNA Infected cell RNA was translated using rabbit reticulocyte lysates (18).…”

Section: Oligodeoxyribonucleotide Primermentioning

confidence: 99%

Nonviral heterogeneous sequences are present at the 5′ ends of one species of snowshoe hare bunyavirus S complementary RNA

Bishop¹,

Gay²,

Matsuoko³

1983

Nucl Acids Res

119

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Analyses of the 5' ends of snowshoe hare bunyavirus plus sense S RNA species (including mRNA) recovered from infected cells have revealed two types of termini. These include ends that are essentially exact copies of the 3' end of the viral S RNA and others that are similar, but additionally have 13-14 nucleotide extensions that are heterogeneous in sequence. The former probably represent replicative plus sense RNA species, the latter mRNA species that have host cell derived primer sequences.

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Section: Oligodeoxyribonucleotide Primermentioning

confidence: 99%

Nonviral heterogeneous sequences are present at the 5′ ends of one species of snowshoe hare bunyavirus S complementary RNA

Bishop¹,

Gay²,

Matsuoko³

1983

Nucl Acids Res

119

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“…Glu (E) Total nurber of residues = 95; M4 10,478 protein NSs (2,(6)(7)(8). A review of the S RNA sequence reveals that in the non-contiguous regions of the genrxe.…”

Section: Characteristics Of the Ssh S Clone 17 And The Predicd S Rnn mentioning

confidence: 99%

The complete sequence and coding content of snowshoe hare bunyavirus small (S) viral RNA species

et al. 1982

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The complete sequence of the small (S) viral RNA species of snowshoe hare (SSH) bunyavirus has been determined, principally from a DNA copy of the RNA cloned in the E.coli plasmid pBr322. The viral S RNA (negative sense strand) is 982 nucleotides long (3.3 x 10(5) daltons) with complementary 5' and 3' end sequences. It has a base composition of 30.5%U, 25.8%A, 24.9%C and 18.7%G. In the viral complementary (plus sense) strand there are two overlapping open reading frames initiated by methionine codons. One reading frame codes for a 26.8 x 10(3) dalton protein, the other for a 10.5 x 10(3) dalton protein. The larger gene product is presumably related to the viral nucleoprotein (N) that is coded by the S RNA (Gentsch and Bishop (1978) J. Virol. 28, 417-419). The smaller gene product is probably related to the recently identified S RNA coded nonstructural protein (NSS) induced in virus infected cells (Fuller and Bishop (1982) J. Virol. 41, 643-648).

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“…Phleboviruses are members of the family Bunyaviridae, which are negative stranded RNA viruses (Cash et al, 1979). They are important pathogens in the Mediterranean area, parts of Africa, and South America (Sabin, 1948;Travassos da Rosa et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Prophylactic and therapeutic activities of 7-thia-8-oxoguanosine against Punta Toro virus infections in mice

et al. 1991

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The biological response modifier 7-thia-8-oxoguanosine was evaluated in mice against the hepatotropic Adames strain of Punta Toro virus. When administered intraperitoneally in divided doses, significant protection from death was conferred at doses of 50 and 100 mg/kg/day given 24 and 17 h pre-virus inoculation, 25-100 mg/kg/day administered 4 h pre- and 3 h post-virus challenge, and 12.5 to 100 mg/kg/day administered 24 and 31 h after virus inoculation. These doses preventing death reduced liver icterus scores, serum alanine aminotransferase and aspartate aminotransferase levels, and liver and serum virus titers relative to placebo controls. Full daily doses administered at 24 h were somewhat less protective to mice than divided daily doses starting at the same time. The initiation of treatment could be delayed as late as 36 h after virus inoculation, resulting in complete protection from mortality at 100 mg/kg/day. This prevention of death occurred despite the acute nature of the infection which resulted in deaths by 96 h in the placebo-treated controls. These results show that 7-thia-8-oxoguanosine has both prophylactic and therapeutic potential as an anti-Phlebovirus agent. Interferon induction appears to be the reason for antiviral activity in this model, since up to 10,000 units of interferon/ml were induced in mice 1 h after treatment with 100 mg 7-thia-8-oxoguanosine per kg, and antibody to interferon alpha/beta administered shortly after treatment with the nucleoside negated the antiviral effect.

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Genome complexities of the three mRNA species of snowshoe hare bunyavirus and in vitro translation of S mRNA to viral N polypeptide

Cited by 48 publications

References 15 publications

Nonviral heterogeneous sequences are present at the 5′ ends of one species of snowshoe hare bunyavirus S complementary RNA

Nonviral heterogeneous sequences are present at the 5′ ends of one species of snowshoe hare bunyavirus S complementary RNA

The complete sequence and coding content of snowshoe hare bunyavirus small (S) viral RNA species

Prophylactic and therapeutic activities of 7-thia-8-oxoguanosine against Punta Toro virus infections in mice

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