Genome Sequence of Pasteurella multocida subsp. gallicida Anand1_poultry

Ahir, Viral B.; Roy, Arkaprovo; Jhala, M. K.; Bhanderi, B. B.; Mathakiya, R. A.; Bhatt, Vaibhav D.; Padiya, Ketan B.; Jakhesara, Subhash J.; Koringa, Prakash G.; Joshi, Chaitanya G.

doi:10.1128/jb.05706-11

Cited by 12 publications

(6 citation statements)

References 7 publications

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“…Comparison of available P. multocida sequences suggests that the presence of this region may be a defining feature of pathogenic avian-source P. multocida strains, as it was present in P1059, X73, and P. multocida subsp. gallicida strain Anand1 isolated from a chicken in India [47] but absent from strains Pm70, pathogenic bovine-source strain 36950 [48] and pathogenic swine source strains 3480 and HN06 [49]. Other studies have demonstrated an ability of avian-source P. multocida to ferment L-fucose, further suggesting that the majority of avian-source P. multocida strains harbor this system [9,33,50].…”

Section: Resultsmentioning

confidence: 99%

Comparative genome analysis of an avirulent and two virulent strains of avian Pasteurella multocida reveals candidate genes involved in fitness and pathogenicity

et al. 2013

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BackgroundPasteurella multocida is the etiologic agent of fowl cholera, a highly contagious and severe disease of poultry causing significant mortality and morbidity throughout the world. All types of poultry are susceptible to fowl cholera. Turkeys are most susceptible to the peracute/acute forms of the disease while chickens are most susceptible to the acute and chronic forms of the disease. The whole genome of the Pm70 strain of P. multocida was sequenced and annotated in 2001. The Pm70 strain is not virulent to chickens and turkeys. In contrast, strains X73 and P1059 are highly virulent to turkeys, chickens, and other poultry species. In this study, we sequenced the genomes of P. multocida strains X73 and P1059 and undertook a detailed comparative genome analysis with the avirulent Pm70 strain. The goal of this study was to identify candidate genes in the virulent strains that may be involved in pathogenicity of fowl cholera disease.ResultsComparison of virulent versus avirulent avian P. multocida genomes revealed 336 unique genes among the P1059 and/or X73 genomes compared to strain Pm70. Genes of interest within this subset included those encoding an L-fucose transport and utilization system, several novel sugar transport systems, and several novel hemagglutinins including one designated PfhB4. Additionally, substantial amino acid variation was observed in many core outer membrane proteins and single nucleotide polymorphism analysis confirmed a higher dN/dS ratio within proteins localized to the outer membrane.ConclusionsComparative analyses of highly virulent versus avirulent avian P. multocida identified a number of genomic differences that may shed light on the ability of highly virulent strains to cause disease in the avian host, including those that could be associated with enhanced virulence or fitness.

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Section: Resultsmentioning

confidence: 99%

Comparative genome analysis of an avirulent and two virulent strains of avian Pasteurella multocida reveals candidate genes involved in fitness and pathogenicity

et al. 2013

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“…For example, a P. multocida B: 2 strain can kill cattle and buffaloes at a low dose, but it has no effect on chickens, even at very high doses (Aktories et al, 2012 ). Isolates from non-avian hosts generally do not cause symptoms of fowl cholera in birds (Ahir et al, 2011 ; Peng et al, 2017 ). However, little is known about the genetic characteristics of P. multocida strains circulating in different hosts.…”

Section: Introductionmentioning

confidence: 99%

Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species

et al. 2018

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Pasteurella multocida is a leading cause of respiratory diseases in many host species. To understand the genetic characteristics of P. multocida strains isolated from different host species, we sequenced the genomic DNA of P. multocida isolated from pigs and analyzed the genetic characteristics of strains from avian species, bovine species, pigs, and rabbits using whole genome sequence (WGS) data. Our results found that a capsular: lipopolysaccharide (LPS): multilocus sequence typing (MLST) genotype A: L1: ST129 (43.75%) was predominant in avian P. multocida; while genotypes B: L2: ST122 (60.00%) and A: L3: ST79 (30.00%) were predominate in bovine P. multocida; genotype D: L6: ST50 (37.50%) in porcine P. multocida; and genotype A: L3: ST9 (76.47%) in rabbit P. multocida. Comparative genomic analysis of P. multocida from different host species found that there are no genes in the P. multocida genome that are specific to any type of host. Phylogenetic analysis using either whole-genome single nucleotide polymorphisms (SNPs) or the set of SNPs present in all single-copy core genes across genomes showed that P. multocida strains with the same LPS genotype and MLST genotype were clustered together, suggesting the combining both the LPS and MLST typing schemes better explained the topology seen in the P. multocida phylogeny.

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“…Among them, serotype A tends to have a wide host range ( 2 , 3 ). However strains isolated from non-avian hosts generally do not infect poultry, and the underling mechanisms are poorly understood ( 23 ). An avian P. multocida of serotype A, PmQ, isolated from tissues of a dead duck shows high virulence to poultry as well as to mammals.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Chicken Lungs Infected With Avian and Bovine Pasteurella multocida Serotype A

et al. 2020

Front. Vet. Sci.

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Pasteurella multocida (P. multocida) is a common animal pathogen responsible for many animal diseases. Strains from different hosts exhibit disparate degrees of effect in other species. Here, we characterize an avian P. multocida serogroup A strain (PmQ) showing high lethality to chickens and a bovine P. multocida serogroup A strain (PmCQ2) with no lethality to chickens. We used RNA-seq to profile the transcriptomes of chicken lungs infected with PmQ and PmCQ2. A total of 1,649 differentially expressed genes (DEGs) due to PmQ infection (831 upregulated genes and 818 downregulated genes) and 1427 DEGs (633 upregulated genes and 794 downregulated genes) due to PmCQ2 infection were identified. Functional analysis of these DEGs demonstrated that the TNF signaling pathway, the toll-like receptor signaling pathway, complement and coagulation cascades, and cytokine-cytokine receptor interaction were both enriched in PmQ and PmCQ2 infection. STAT and apoptosis signaling pathways were uniquely enriched by PmQ infection, and the NOD-like receptor signaling pathway was enriched only by PmCQ2 infection. Cell-type enrichment analysis of the transcriptomes showed that immune cells, including macrophages and granulocytes, were enriched in both infection groups. Collectively, our study profiled the transcriptomic response of chicken lungs infected with P. multocida and provided valuable information to understand the chicken responses to P. multocida infection.

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Genome Sequence of Pasteurella multocida subsp. gallicida Anand1_poultry

Cited by 12 publications

References 7 publications

Comparative genome analysis of an avirulent and two virulent strains of avian Pasteurella multocida reveals candidate genes involved in fitness and pathogenicity

Comparative genome analysis of an avirulent and two virulent strains of avian Pasteurella multocida reveals candidate genes involved in fitness and pathogenicity

Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species

Transcriptomic Analysis of Chicken Lungs Infected With Avian and Bovine Pasteurella multocida Serotype A

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