Genome sequences of two Antarctic strains of Pseudomonas prosekii: insights into adaptation to extreme conditions

Snopková, Kateřina; Čejková, Darina; Dufková, Kristýna; Sedláček, Ivo; Šmajs, David

doi:10.1007/s00203-019-01755-4

Cited by 8 publications

(12 citation statements)

References 39 publications

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“…We recently identified prosekin of P. prosekii LMG 26867 T of the Mandelii SG as a fourth member synthesized by a quite divergent NRPS system ( 19 ). Next to the P. prosekii type strain and coisolated strains originating from the Antarctic region ( 35 , 36 ), strain AALPS.10.MNAAK.13 isolated from the Arctic region carries a prosekin BGC (Table S4A) ( 37 ). However, this BGC is not confined to such cold-adapted strains as it is also present in bark beetle-associated isolate A2-NA12 ( 38 ).…”

Section: Resultsmentioning

confidence: 99%

Charting the Lipopeptidome of Nonpathogenic Pseudomonas

et al. 2023

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Pseudomonas species are prominent producers of lipopeptides that support proliferation in a multitude of environments and foster varied lifestyles. By genome mining of biosynthetic gene clusters (BGCs) with lipopeptide-specific organization, we mapped the global Pseudomonas lipopeptidome and linked its staggering diversity to taxonomy of the producers, belonging to different groups within the major Pseudomonas fluorescens lineage.

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Section: Resultsmentioning

confidence: 99%

Charting the Lipopeptidome of Nonpathogenic Pseudomonas

et al. 2023

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“…Bacterial adaptations to facilitate survival at low temperatures include cell membrane fluidity (increased unsaturated fatty acids) and protein-specific responses (refolding cold-damaged proteins) 12 . Cold-shock proteins (Csp) are produced by bacteria in response to a rapid decrease in temperature and act as RNA chaperones to help prevent mRNA misfolding 13 . The PGAP analysis in the current study showed that the AK-R2A1-2 T genome contained two genes encoding a cold-shock protein (UFS59601.1; UFS60376.1) and a cold shock domain-containing protein (UFS59609.1).…”

Section: Resultsmentioning

confidence: 99%

Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana

Jiang

Peng

Seo

et al. 2022

Sci Rep

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A novel Gram-stain-positive, aerobic bacterial strain, designated AK-R2A1-2 T, was isolated from the surface-sterilized needle leaves of an Abies koreana tree. Strain AK-R2A1-2 T had 97.3% and 96.7% 16S rRNA gene sequence similarities with Subtercola boreus K300T and Subtercola lobariae 9583bT, respectively, but formed a distinct phyletic lineage from these two strains. Growth of strain AK-R2A1-2 T was observed at 4–25 °C at pH 5.0–8.0. Strain AK-R2A1-2 T contained menaquinone 9 (MK-9) and menaquinone 10 (MK-10) as the predominant respiratory quinones. The major cellular fatty acids were anteiso-C15:0 and summed feature 8 (C18:1ω7c or/and C18:1ω6c), and the polar lipids included diphosphatidylglycerol (DPG) and three unknown aminolipids, AKL2, AKL3, and AKL4. The complete genome of strain AK-R2A1-2 T was sequenced to understand the genetic basis of its survival at low temperatures. Multiple copies of cold-associated genes involved in cold-active chaperon, stress response, and DNA repair supported survival of the strain at low temperatures. Strain AK-R2A1-2 T was also able to significantly improve rice seedling growth under low temperatures. Thus, this strain represents a novel species of the genus Subtercola, and the proposed name is Subtercola endophyticus sp. nov. The type strain is AK-R2A1-2 T (= KCTC 49721 T = GDMCC 1.2921 T).

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“…Many strains of Pseudomonas sp. are known to produce antimicrobial agents (Ghequire & De Mot, 2014; Kerbauy et al, 2016; Snopková et al, 2019). 11 BGCs were identified in the Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

Genomic features of Pseudomonas sp. RGM2144 correlates with increased survival of rainbow trout infected by Flavobacterium psychrophilum

Vidal

Fonseca

Ruiz

et al. 2022

Journal of Fish Diseases

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This study evaluated the probiotic potential of the biofilm formed by the strain Pseudomonas sp. RGM2144 on rainbow trout survival. When challenged with the fish pathogen Flavobacterium psychrophilum, Pseudomonas sp. RGM2144 increased rainbow trout survival to 92.7 ± 1.2% (control: 35.3 ± 9.5%, p < .0001). The draft genome of Pseudomonas sp. RGM2144 is 6.8 Mbp long, with a completeness 100% and a contamination of 0.4%. The genome contains 6122 protein-coding genes of which 3564 (~60%) have known functions. The genome and phylogeny indicate that Pseudomonas sp. RGM2144 is a new species in the Pseudomonas genus, with few virulence factors, plasmids, and genes associated with antimicrobial resistance, suggesting a nonpathogenic bacterium with protective potential. In addition, the genome encodes for 11 secondary metabolite biosynthetic gene clusters that could be involved in the inhibition of F. psychrophilum. We suggest that Pseudomonas sp. RGM2144 may be applied as a probiotic in salmonid fish farming.

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Genome sequences of two Antarctic strains of Pseudomonas prosekii: insights into adaptation to extreme conditions

Cited by 8 publications

References 39 publications

Charting the Lipopeptidome of Nonpathogenic Pseudomonas

Charting the Lipopeptidome of Nonpathogenic Pseudomonas

Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana

Genomic features of Pseudomonas sp. RGM2144 correlates with increased survival of rainbow trout infected by Flavobacterium psychrophilum

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