Genome-wide analysis indicates diverse physiological roles of the turnip (Brassica rapa var. rapa) oligopeptide transporters gene family

Abstract

Oligopeptide transporters (OPTs) encode integral membrane-localized proteins and have a broad range of substrate transport capabilities. Here, 28 BrrOPT genes were identified in the turnip. Phylogenetic analyses revealed two well-supported clades in the OPT family, containing 15 BrrOPTs and 13 BrrYSLs. The exon/intron structure of OPT clade are conserved but the yellow stripe-like (YSL) clade was different. The exon/intron of the YSL clade possesses structural differences, whereas the YSL class motifs structure are conserved. The OPT genes are distributed unevenly among the chromosomes of the turnip genome. Phylogenetic and chromosomal distribution analyses revealed that the expansion of the OPT gene family is mainly attributable to segmental duplication. For the expression profiles at different developmental stages, a comprehensive analysis provided insights into the possible functional divergence among members of the paralog OPT gene family. Different expression levels under a variety of ion deficiencies also indicated that the OPT family underwent functional divergence during long-term evolution. Furthermore, BrrOPT8.1, BrrYSL1.2, BrrYSL1.3, BrrYSL6 and BrrYSL9 responded to Fe(II) treatments and BrrYSL7 responded to calcium treatments, BrrYSL6 responded to multiple treatments in root, suggesting that turnip OPTs may be involved in mediating cross-talk among different ion deficiencies. Our data provide important information for further functional dissection of BrrOPTs, especially in transporting metal ions and nutrient deficiency stress adaptation.

Genome-Wide Identification and Transcript Analysis Reveal Potential Roles of Oligopeptide Transporter Genes in Iron Deficiency Induced Cadmium Accumulation in Peanut

“…The majority of AhOPT proteins shared similar physicochemical properties ( Table 1 ). Overall, most AhOPTs are basic and hydrophobic proteins with high in vitro stability over a wide temperature range, which concurred with those obtained from other plant species (Cao et al, 2011 ; Pu et al, 2018 ; Su et al, 2019 ). All AhOPT proteins were predicted to be localized in plasma membranes ( Table 1 ).…”

“…Herein, 40 putative AhOPT genes were identified in peanut ( Table 1 ). The number of peanut AhOPT genes is higher than that of several reported plant species, such as Arabidopsis (17) (Koh et al, 2002 ), rice (27) (Vasconcelos et al, 2008 ), poplar (20) (Cao et al, 2011 ), grape (18) (Cao et al, 2011 ), turnip (28) (Pu et al, 2018 ), and ginseng (37) (Su et al, 2019 ). However, it is greatly smaller than that of hexaploid wheat (107) (Kumar et al, 2019 ).…”

“…Genome-wide identification of the OPT family has been performed in several plant species, including poplar ( Populus trichocarpa ) (Cao et al, 2011 ), grape ( Vitis vinifera ) (Cao et al, 2011 ), ginseng ( Panax ginseng ) (Su et al, 2019 ), turnip ( Brassica rapa var. rapa ) (Pu et al, 2018 ), and wheat (Kumar et al, 2019 ). However, there is little information about the OPT family in peanut.…”

Evolutionary Expansion and Expression Analysis of the Oligopeptide Transporter Gene Family Between Cucurbita Moschata and Cucurbita Maxima

“…The expression pro les of family members in crops can provide useful clues for gene function [25]. In this study, the expression levels of 45 OPT genes were analyzed using publicly available RNA sequence data from the genomes of Cucurbita moschata and Cucurbita maxima [27].…”

“…With the development of High-throughput sequencing technology in recent years, more and more plant species have been sequenced, and the characteristics of OPTs family genes have also been described, such as Ganoderma lucidum [23][24][25][26] and so on. The growth and development of crops are often affected by external biotic and abiotic environmental stresses, especially abiotic stresses (drought, high temperature, salt stress, freezing injury, etc.).…”