Genome-wide comparison of four MRSA clinical isolates from Germany and Hungary

Naorem, Romen Singh; Blom, Jochen; Fekete, Csaba

doi:10.7717/peerj.10185

Cited by 8 publications

(14 citation statements)

References 126 publications

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“…In addition, when investigating phage-antibiotic synergy, many studies rely on single or few reference strains or clinical isolates only as being representative for the whole species. However, given the genetic and phenotypic heterogeneity of opportunistic pathogens and even laboratory strains [16][17][18][19], it is important to test the suitability of potential phage-antibiotic pairs on a larger set of bacterial isolates. In this study, we tested the hypothesis that oxacillin, the antibiotic of choice against Methicillin-susceptible S. aureus (MSSA), and phage Sb-1, a member of the Twort-like viruses, exert stronger activity against MRSA in combination than either antimicrobial substance alone.…”

Section: Introductionmentioning

confidence: 99%

Synergy between Phage Sb-1 and Oxacillin against Methicillin-Resistant Staphylococcus aureus

et al. 2021

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Methicillin-resistant Staphylococcus aureus (MRSA) is a notorious pathogen responsible for not only a number of difficult-to-treat hospital-acquired infections, but also for infections that are community- or livestock-acquired. The increasing lack of efficient antibiotics has renewed the interest in lytic bacteriophages (briefly phages) as additional antimicrobials against multi-drug resistant bacteria, including MRSA. The aim of this study was to test the hypothesis that a combination of the well-known and strictly lytic S. aureus phage Sb-1 and oxacillin, which as sole agent is ineffective against MRSA, exerts a significantly stronger bacterial reduction than either antimicrobial alone. Eighteen different MRSA isolates and, for comparison, five MSSA and four reference strains were included in this study. The bacteria were challenged with a combination of varying dosages of the phage and the antibiotic in liquid medium using five different antibiotic levels and four different viral titers (i.e., multiplicity of infections (MOIs) ranging from 10−5 to 10). The dynamics of the cell density changes were determined via time-kill assays over 16 h. Positive interactions between both antimicrobials in the form of facilitation, additive effects, or synergism were observed for most S. aureus isolates. These enhanced antibacterial effects were robust with phage MOIs of 10−1 and 10 irrespective of the antibiotic concentrations, ranging from 5 to 100 µg/mL. Neutral effects between both antimicrobials were seen only with few isolates. Importantly, antagonism was a rare exception. As a conclusion, phage Sb-1 and oxacillin constitute a robust heterologous antimicrobial pair which extends the efficacy of a phage-only approach for controlling MRSA.

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Section: Introductionmentioning

confidence: 99%

Synergy between Phage Sb-1 and Oxacillin against Methicillin-Resistant Staphylococcus aureus

et al. 2021

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“…Among the 65 analyzed prophages, 57 prophages were extracted from methicillin-resistance S. aureus (MRSA) strains, while the 8 analyzed prophages were extracted from methicillin-sensitive S. aureus (MSSA) strains in which 4 prophages were from 4 MSSA strains ( S. aureus HD1410, S. aureus I3, S. aureus SA13-192, and S. aureus SA14-639)-associated with human infections, and another 4 prophages were extracted from 4 MSSA strains ( S. aureus 483, S. aureus 909, S. aureus C3489, and S. aureus C5086)-associated with bovine and dog infections. Among the MRSA strains, SA G6, and S. SA G8 strains were reported earlier as hospital-associated MRSA (HA-MRSA) 25 , while the other MRSA strains carried Staphylococcal Cassette Chromosome mec ( SCCmec ) types IV, and V which are regarded as community-associated-MRSA (CA-MRSA) 26 . The MLST analysis result revealed that S. aureus strains associated with the animal infections have sequence types ST398 (clonal complex, CC398) and ST151 (CC151), while the S. aureus strains associated with human infections have ST398 (CC398), ST8 (CC8), ST5 (CC5), etc.…”

Section: Resultsmentioning

confidence: 99%

“…The clades were shaded with different colors and the prophage names labeled in black, red, and blue colors represent the prophages extracted from the S. aureus associated with Homo sapiens , Bos tourus , and Canis lupus familiaris , respectively. And the prophage names labeled in green color represent the prophages extracted from our previous study 25 , 27 . …”

Section: Resultsmentioning

confidence: 99%

“…

Figure 3 The mosaic architecture of S. aureus prophage genomes. ( A ) Comparison of prophages of clade 1, representing phiG5-3, and phiH14-1 sequenced of our previous study 25 and 7 prophages associated with S. aureus reported from Germany, Austria, France, and Netherlands; ( B ) Comparison of prophages of clade 1, representing prophages of S. aureus associated with Homo sapiens and Bos tourus ; ( C ) Comparison of prophages of clade 3, representing prophages phiG5-5 & phiH14-2 (subclade 3B) sequenced of our previous study and 6 prophages associated with S. aureus reported from Denmark, France, Spain, Germany, Italy, and Hungary; ( D ) Comparison of prophages in clade 3, representing prophages phiG4-3 (subclade 3C) sequenced of our previous study and 6 prophages (subclade 3A) associated with S. aureus spreading in France, Spain, and Germany. Phages and country of origins are indicated on the right (Prophage names labeled in black, red, and blue color indicated prophages associated with Homo sapiens , Bos tourus, and Canis lupus familiaris respectively.…”

Section: Resultsmentioning

confidence: 99%

“…

Figure 4 The mosaic architecture of S. aureus prophage genomes. ( A ) Comparison of prophages of clade 5, representing the prophages of subclade 5A, and phiH13-2 sequenced of our previous study 27 reported from Austria, Hungary, Canada (reference prophage), and Germany; ( B ) Comparison of prophages of clade 6, representing prophages of Homo sapiens and Bos tourus - associated S. aureus spreading in Germany, Austria, Denmark, Netherlands, Japan (reference prophage), and Italy; ( C ) Comparison of prophages of clade 7, representing subclade 7A prophages Homo sapiens , Bos tourus, and Canis lupus familiaris -associated S. aureus reported from Netherland, Spain, and France; ( D ) Comparison of prophages of clade 7, representing phiG5-2 prophage sequence of our previous study 25 and 3 prophages of subclade 7B associated with S. aureus reported from Italy, and Netherland. Phages and country of origins are indicated on the right (the prophage names labeled in black, red, blue, and purple color indicate prophages associated with Homo sapiens , Bos tourus, Canis lupus familiaris, and reference, respectively.…”

Section: Resultsmentioning

confidence: 99%

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Comparative analysis of prophages carried by human and animal-associated Staphylococcus aureus strains spreading across the European regions

Naorem

Goswami²,

Schneider

et al. 2021

Sci Rep

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Staphylococcus aureus is a major human and animal pathogen although the animal-associated S. aureus can be a potential risk of human zoonoses. Acquisition of phage-related genomic islands determines the S. aureus species diversity. This study characterized and compared the genome architecture, distribution nature, and evolutionary relationship of 65 complete prophages carried by human and animal-associated S. aureus strains spreading across the European regions. The analyzed prophage genomes showed mosaic architecture with extensive variation in genome size. The phylogenetic analyses generated seven clades in which prophages of the animal-associated S. aureus scattered in all the clades. The S. aureus strains with the same SCCmec type, and clonal complex favored the harboring of similar prophage sequences and suggested that the frequency of phage-mediated horizontal gene transfer is higher between them. The presence of various virulence factors in prophages of animal-associated S. aureus suggested that these prophages could have more pathogenic potential than prophages of human-associated S. aureus. This study showed that the S. aureus phages are dispersed among the several S. aureus serotypes and around the European regions. Further, understanding the phage functional genomics is necessary for the phage-host interactions and could be used for tracing the S. aureus strains transmission.

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Prevalence and antimicrobial resistance profiling of Staphylococcus aureus isolated from traditional cheese in Yunnan, China

et al. 2021

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The prevalence of staphylococcal infection and the emergence of multidrug resistance of Staphylococcus aureus (S. aureus) are major concerns in food safety and public health. This study aimed to investigate the prevalence of S. aureus isolated from traditional Chinese Rubing and Rushan cheese, antimicrobial resistance profiles, genomic characteristics, and predict antimicrobial resistance genes (ARGs). From 124 samples, 18 of 62 (29.03%) of Rubing and 5 of 62 (8.06%) of Rushan cheese were confirmed to be S. aureus positive by standard culture-based methods. Twenty-three coagulase-positive staphylococci isolates were grouped into 16 clusters by pulsed-field gel electrophoresis and subjected to routine susceptibility testing to 12 antibiotics. Those isolates exhibited high resistance to penicillin (100%), erythromycin, trimethoprim-sulphamethoxazole (34.78%), oxacillin, clindamycin, and cefoxitin (21.74%). Multidrug-resistant (MDR) S. aureus was found in 34.78% (8 of 23) of isolates. Further, S. aureus strain DC.RB_015 isolated from Rubing cheese, recognized as the most resistant to six antibiotics, was selected for whole-genome sequencing (WGS), continued with in silico approaches. S. aureus DC.RB_015 had a single chromosome size of 2,794,578 bp and a plasmid size of 22,961 bp. The strain harbored 18 predicted ARGs, including eight efflux pump genes (mepA, tet(K), arlR, arlS, norA, mgrA, tet(38), LmrS), one peptidoglycan biosynthesis gene (bacA), two β-lactams resistance genes (mecA, blaZ), and seven genes conferring other antimicrobial resistance (APH(3′)-IIIa, aad(6), ErmB, mecR1, GlpT, murA). The results of this study expand the knowledge of S. aureus strain DC.RB_015, increase food safety awareness, and will be helpful in establishing therapeutic therapy.

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Genome-wide comparison of four MRSA clinical isolates from Germany and Hungary

Cited by 8 publications

References 126 publications

Synergy between Phage Sb-1 and Oxacillin against Methicillin-Resistant Staphylococcus aureus

Synergy between Phage Sb-1 and Oxacillin against Methicillin-Resistant Staphylococcus aureus

Comparative analysis of prophages carried by human and animal-associated Staphylococcus aureus strains spreading across the European regions

Prevalence and antimicrobial resistance profiling of Staphylococcus aureus isolated from traditional cheese in Yunnan, China

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