Genome-wide diversity analysis of TCP transcription factors revealed cases of selection from wild to cultivated barley

Gao, Guang; Kan, Jinhong; Jiang, Congcong; Ahmar, Sunny; Zhang, Jing; Yang, Ping

doi:10.1007/s10142-020-00759-4

Cited by 9 publications

(9 citation statements)

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“…Phylogenetic analysis and sequence alignment showed that OpTCPs in the same group or subgroup shared similar motifs composition and gene structures (Figure 3), which was consistent with the previously described in Arabidopsis (Li, 2015), maize (Ding et al, 2019), potato (Bao et al, 2019), and barley (Gao et al, 2021). For example, motif 2 in N-terminal TCP domain was only present in all CIN subclade, while the conserved R domain (motif 3) was not detected in the PCF subclass (Figure 3C).…”

Section: Discussionsupporting

confidence: 86%

“…To date, genome-wide identification of the TCP family members has been identified and characterized in many dicots and monocots plants, such as A. thaliana (Li, 2015), Solanum lycopersicum (Parapunova et al, 2014), Malus domestica (Xu et al, 2014), Citrullus lanatus (Shi et al, 2016), Nicotiana tabacum (Chen et al, 2016), Fragaria vesca (Wei et al, 2016), Glycine max (Feng et al, 2018), Gossypium barbadense (Zheng et al, 2018), Vitis vinifera (Leng et al, 2019), Solanum tuberosum (Bao et al, 2019), Phyllostachys edulis (Liu et al, 2018), Z. mays (Ding et al, 2019), Panicum virgatum (Zheng et al, 2019), Hordeum vulgare subsp. vulgare (Gao et al, 2021), and so on. However, there is no research about the identification and functional characterization of TCP proteins in CPT-producing plants.…”

Section: Introductionmentioning

confidence: 98%

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Genome-Wide Identification and Comparative Analysis of the Teosinte Branched 1/Cycloidea/Proliferating Cell Factors 1/2 Transcription Factors Related to Anti-cancer Drug Camptothecin Biosynthesis in Ophiorrhiza pumila

et al. 2021

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Ophiorrhiza pumila (O. pumila; Op) is a medicinal herbaceous plant, which can accumulate camptothecin (CPT). CPT and its derivatives are widely used as chemotherapeutic drugs for treating malignant tumors. Its biosynthesis pathway has been attracted significant attention. Teosinte branched 1/cycloidea/proliferating cell factors 1/2 (TCP) transcription factors (TFs) regulate a variety of physiological processes, while TCP TFs are involved in the regulation of CPT biosynthesis remain unclear. In this study, a systematic analysis of the TCP TFs family in O. pumila was performed. A total of 16 O. pumila TCP (OpTCP) genes were identified and categorized into two subgroups based on their phylogenetic relationships with those in Arabidopsis thaliana. Tissue-specific expression patterns revealed that nine OpTCP genes showed the highest expression levels in leaves, while the other seven OpTCPs showed a higher expression level in the stems. Co-expression, phylogeny analysis, and dual-luciferase (Dual-LUC) assay revealed that OpTCP15 potentially plays important role in CPT and its precursor biosynthesis. In addition, the subcellular localization experiment of candidate OpTCP genes showed that they are all localized in the nucleus. Our study lays a foundation for further functional characterization of the candidate OpTCP genes involved in CPT biosynthesis regulation and provides new strategies for increasing CPT production.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 98%

Genome-Wide Identification and Comparative Analysis of the Teosinte Branched 1/Cycloidea/Proliferating Cell Factors 1/2 Transcription Factors Related to Anti-cancer Drug Camptothecin Biosynthesis in Ophiorrhiza pumila

et al. 2021

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“…Similar to the situation in other plants, barley TCPs grouped into two main classes, class I (PCF) and class II (CIN/CYC/TB1) (Fig. 1), confirming the results of a recent analysis (Gao et al 2021). Overall, TCPs were expressed in various tissue types and developmental stages (Supplementary Fig.…”

Section: Barley Class II Tcps Have a Grass-specific Sister Clade Of Tb1supporting

confidence: 87%

The TCP transcription factor HvTB2 heterodimerizes with VRS5 and controls spike architecture in barley

Moraes

Hernández-Pinzón

et al. 2022

Plant Reprod

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Key message Understanding the molecular network, including protein-protein interactions, of VRS5 provide new routes towards the identification of other key regulators of plant architecture in barley. Abstract The TCP transcriptional regulator TEOSINTE BRANCHED 1 (TB1) is a key regulator of plant architecture. In barley, an important cereal crop, HvTB1 (also referred to as VULGARE SIX-ROWED spike (VRS) 5), inhibits the outgrowth of side shoots, or tillers, and grains. Despite its key role in barley development, there is limited knowledge on the molecular network that is utilized by VRS5. In this work, we performed protein–protein interaction studies of VRS5. Our analysis shows that VRS5 potentially interacts with a diverse set of proteins, including other class II TCP’s, NF-Y TF, but also chromatin remodelers. Zooming in on the interaction capacity of VRS5 with other TCP TFs shows that VRS5 preferably interacts with other class II TCP TFs in the TB1 clade. Induced mutagenesis through CRISPR–Cas of one of the putative VRS5 interactors, HvTB2 (also referred to as COMPOSITUM 1 and BRANCHED AND INDETERMINATE SPIKELET 1), resulted in plants that have lost their characteristic unbranched spike architecture. More specifically, hvtb2 mutants exhibited branches arising at the main spike, suggesting that HvTB2 acts as inhibitor of branching. Our protein–protein interaction studies of VRS5 resulted in the identification of HvTB2 as putative interactor of VRS5, another key regulator of spike architecture in barley. The study presented here provides a first step to underpin the protein–protein interactome of VRS5 and to identify other, yet unknown, key regulators of barley plant architecture.

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“…This is due to the presence of three fertile spikelets, one central and two lateral, at each node, while in two-rowed barley the development of the two lateral spikelets is inhibited [120]. Selection of specific INT-C alleles was probably involved in the development of six-rowed varieties during barley domestication [121]. The sorghum (Sorghum bicolor) CYC/TB1 transcription factor MULTISEEDED 1 (MSD1) is also involved in the control of spikelet fertility [122].…”

Section: Tb1-like Tcps and The Regulation Of Inflorescence Architectu...mentioning

confidence: 99%

TCP Transcription Factors in Plant Reproductive Development: Juggling Multiple Roles

Viola

González

2023

Biomolecules

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TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factors (TFs) are plant-specific transcriptional regulators exerting multiple functions in plant growth and development. Ever since one of the founding members of the family was described, encoded by the CYCLOIDEA (CYC) gene from Antirrhinum majus and involved in the regulation of floral symmetry, the role of these TFs in reproductive development was established. Subsequent studies indicated that members of the CYC clade of TCP TFs were important for the evolutionary diversification of flower form in a multitude of species. In addition, more detailed studies of the function of TCPs from other clades revealed roles in different processes related to plant reproductive development, such as the regulation of flowering time, the growth of the inflorescence stem, and the correct growth and development of flower organs. In this review, we summarize the different roles of members of the TCP family during plant reproductive development as well as the molecular networks involved in their action.

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Genome-wide diversity analysis of TCP transcription factors revealed cases of selection from wild to cultivated barley

Cited by 9 publications

References 64 publications

Genome-Wide Identification and Comparative Analysis of the Teosinte Branched 1/Cycloidea/Proliferating Cell Factors 1/2 Transcription Factors Related to Anti-cancer Drug Camptothecin Biosynthesis in Ophiorrhiza pumila

Genome-Wide Identification and Comparative Analysis of the Teosinte Branched 1/Cycloidea/Proliferating Cell Factors 1/2 Transcription Factors Related to Anti-cancer Drug Camptothecin Biosynthesis in Ophiorrhiza pumila

The TCP transcription factor HvTB2 heterodimerizes with VRS5 and controls spike architecture in barley

TCP Transcription Factors in Plant Reproductive Development: Juggling Multiple Roles

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