Genome-wide genetic diversity of rove beetle populations along a metal pollution gradient

Giska, Iwona; Babik, Wiesław; Gestel, Cornelis A.M. van; Straalen, Nico M. van; Laskowski, Ryszard

doi:10.1016/j.ecoenv.2015.04.048

Cited by 21 publications

(15 citation statements)

References 64 publications

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“…However, either experimental manipulations or field data from multiple pollution gradients are necessary to demonstrate that individuals carrying the mtDNA of the E lineage are more sensitive to metal pollution. On the other hand, among the three contaminated OL sites, the most polluted site OL2 was characterized by relatively high haplotype and nucleotide diversity and the largest number of the polymorphic sites and private SNPs; therefore, this result did not support the genetic erosion hypothesis and was consistent with a pattern we identified also for the rove beetle Staphylinus erythropterus , inhabiting the same gradient [ 58 ].…”

Section: Discussionmentioning

confidence: 57%

Deeply divergent sympatric mitochondrial lineages of the earthworm Lumbricus rubellus are not reproductively isolated

2015

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BackgroundThe accurate delimitation of species is essential to numerous areas of biological research. An unbiased assessment of the diversity, including the cryptic diversity, is of particular importance for the below ground fauna, a major component of global biodiversity. On the British Isles, the epigeic earthworm Lumbricus rubellus, which is a sentinel species in soil ecotoxicology, consists of two cryptic taxa that are differentiated in both the nuclear and the mitochondrial (mtDNA) genomes. Recently, several deeply divergent mtDNA lineages were detected in mainland Europe, but whether these earthworms also constitute cryptic species remains unclear. This information is important from an evolutionary perspective, but it is also essential for the interpretation and the design of ecotoxicological projects. In this study, we used genome-wide RADseq data to assess the reproductive isolation of the divergent mitochondrial lineages of L. rubellus that occur in sympatry in multiple localities in Central Europe.ResultsWe identified five divergent (up to 16 % net p-distance) mitochondrial lineages of L. rubellus in sympatry. Because the clustering of the RADseq data was according to the population of origin and not the mtDNA lineage, reproductive isolation among the mtDNA lineages was not likely. Although each population contained multiple mtDNA lineages, subdivisions within the populations were not observed for the nuclear genome. The lack of fixed differences and sharing of the overwhelming majority of nuclear polymorphisms between localities, indicated that the populations did not constitute allopatric species. The nucleotide diversity within the populations was high, 0.7–0.8 %.ConclusionsThe deeply divergent mtDNA sympatric lineages of L. rubellus in Central Europe were not reproductively isolated groups. The earthworm L. rubellus, which is represented by several mtDNA lineages in continental Europe, apparently is a single highly polymorphic species rather than a complex of several cryptic species. This study demonstrated the critical importance of the use of multilocus nuclear data for the unbiased assessment of cryptic diversity and for the delimitation of species in soil invertebrates.Electronic supplementary materialThe online version of this article (doi:10.1186/s12862-015-0488-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 57%

Deeply divergent sympatric mitochondrial lineages of the earthworm Lumbricus rubellus are not reproductively isolated

2015

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“…Studies of the impacts of contaminants on genetic diversity have increased in recent years owing to the advent of affordable DNA sequencing services and the recognized importance of maintaining genetic diversity in the conservation of wildlife populations (Frankham, Bradshaw, & Brook, 2014;Giska, Babik, Gestel, Straalen, & Laskowski, 2015;Rumisha et al, 2017). Alterations to genetic diversity may lead to reduced susceptibility to environmental change (Ehlers, Worm, & Reusch, 2008) and increased extinction potential.…”

Section: Introductionmentioning

confidence: 99%

“…SNPs are more widely abundant across the genome as compared to microsatellites and RFLP-based techniques and are generally more reproducible (Davey et al, 2011;Schlötterer, 2004). The advent of affordable sequencing coupled with continual advancements in technologies have led to the application of genome-wide SNP approaches to a wide range of questions, including evolutionary history (Pollinger et al, 2010), conservation (Larson et al, 2014), and ecotoxicology (Giska et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Chronic radiation exposure at Chernobyl shows no effect on genetic diversity in the freshwater crustacean, Asellus aquaticus thirty years on

Fuller

Ford

Lerebours

et al. 2019

Ecology and Evolution

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Analysis of genetic diversity represents a fundamental component of ecological risk assessments in contaminated environments. Many studies have assessed the genetic implications of chronic radiation exposure at Chernobyl, generally recording an elevated genetic diversity and mutation rate in rodents, plants, and birds inhabiting contaminated areas. Only limited studies have considered genetic diversity in aquatic biota at Chernobyl, despite the large number of freshwater systems where elevated dose rates will persist for many years. Consequently, the present study aimed to assess the effects of chronic radiation exposure on genetic diversity in the freshwater crustacean, Asellus aquaticus, using a genome‐wide SNP approach (Genotyping‐by‐sequencing). It was hypothesized that genetic diversity in A. aquaticus would be positively correlated with dose rate. A. aquaticus was collected from six lakes in Belarus and the Ukraine ranging in dose rate from 0.064 to 27.1 µGy/hr. Genotyping‐by‐sequencing analysis was performed on 74 individuals. A significant relationship between geographical distance and genetic differentiation confirmed the Isolation‐by‐Distance model. Conversely, no significant relationship between dose rate and genetic differentiation suggested no effect of the contamination gradient on genetic differentiation between populations. No significant relationship between five measures of genetic diversity and dose rate was recorded, suggesting that radiation exposure has not significantly influenced genetic diversity in A. aquaticus at Chernobyl. This is the first study to adopt a genome‐wide SNP approach to assess the impacts of environmental radiation exposure on biota. These findings are fundamental to understanding the long‐term success of aquatic populations in contaminated environments at Chernobyl and Fukushima.

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“…In contrast, metals could accumulate gradually and magnify because of the food chain, finally passing into the human body . A large body of evidence shows the influence of heavy metal ions on the environment, and on water and organisms . These heavy metals have the potential to influence proteins, change biological activity, and accumulate in some organs causing chronic poisoning.…”

Section: Resultsmentioning

confidence: 99%

Probing the interaction of cephalosporin with bovine serum albumin: A structural and comparative perspective

Cheng

Yang

et al. 2017

Luminescence

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Cephalosporins belong the largest class of antibiotics used in the treatment of a wide range of infectious diseases caused by susceptible organisms. In the present study, we chose two typical antibiotics cefalexin/cefixime based on their structure, and investigated the interaction of cephalexin/cefixime with bovine serum albumin (BSA) using UV-vis absorption spectra, fluorescence spectroscopy, circular dichroism (CD) spectroscopy and molecular modeling approaches. Spectroscopic experiments revealed the formation of a BSA − cefalexin/cefixime complex. The binding parameters calculated using a modified Stern − Volmer method and the Scatchard method reached 10 3 -10 4 L·mol −1 . Thermodynamic parameter studies revealed that binding characteristics by negative enthalpy and positive entropy changes, and electrostatic interactions play a major role. Site marker competitive displacement experiments and molecular modeling approaches demonstrated that cefalexin and cefixime bind with appropriate affinity to site I (subdomain IIA) of BSA. Furthermore, synchronous fluorescence spectra, CD spectra and molecular modeling results indicated that the secondary structure of BSA was changed in the presence of cefalexin and cefixime. Additionally, the effects of metal ions on the BSA − cefalexin/cefixime system were also assessed.

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Genome-wide genetic diversity of rove beetle populations along a metal pollution gradient

Cited by 21 publications

References 64 publications

Deeply divergent sympatric mitochondrial lineages of the earthworm Lumbricus rubellus are not reproductively isolated

Deeply divergent sympatric mitochondrial lineages of the earthworm Lumbricus rubellus are not reproductively isolated

Chronic radiation exposure at Chernobyl shows no effect on genetic diversity in the freshwater crustacean, Asellus aquaticus thirty years on

Probing the interaction of cephalosporin with bovine serum albumin: A structural and comparative perspective

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