Genome wide identification and functional characterization of two LC-PUFA biosynthesis elongase (elovl8) genes in rabbitfish (Siganus canaliculatus)

Li, Yang; Wen, Zhengyong; You, Cuihong; Xie, Zhiyong; Tocher, Douglas R.; Zhang, Yueling; Wang, Shuqi; Li, Yuanyou

doi:10.1016/j.aquaculture.2020.735127

Cited by 44 publications

(43 citation statements)

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“…We identified Elovl8 sequences in chordates including teleosts, amphibians, reptiles, birds, and mammals, a clear indication that Elovl8 gene is widely distributed in vertebrates and not exclusively unique to teleosts as previously suggested [31]. Tree topology with the invertebrate Elovl8 out-grouping vertebrate deuterostome Elovl8 clade shows that this gene predates the emergence of vertebrates and is not a result of the duplication of an ancestral Elovl gene in sarcopterygians [31]. We additionally showed that some tetrapods (G. gallus, M. gallopavo, A. carolinensis, A. mississippiensis, and T. sirtalis) have lost the elovl8 gene, while other species like H. sapiens still retain a non-coding elovl8 gene.…”

Section: Discussionsupporting

confidence: 67%

“…Elovl1-Elovl7 have been extensively studied in many metazoans including invertebrates and vertebrates. A novel member of the Elovl family, Elovl8, has recently been described in two teleosts [30,31]. However, the exact role of elovl8 in FA elongation deserves further exploration.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently two zebrafish (Danio rerio) isoforms of this ortholog were termed as elovl8a, b and deposited in GenBank [30]. More recently, full length cDNA of elovl8a and elovl8b were cloned from rabbitfish (Siganus caniculatus) and characterized for their in vitro elongation capacity on different FAs through functional in vitro assay employing heterologous yeast expression [31]. Results showed elovl8a had no activity and elovl8b had lower activity in the elongation of C18 and C20 PUFA substrates.…”

Section: Introductionmentioning

confidence: 99%

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Evolution and Functional Characteristics of the Novel elovl8 That Play Pivotal Roles in Fatty Acid Biosynthesis

Sun

Wang

Goh

et al. 2021

Genes

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Elongation of very long-chain fatty acid (Elovl) proteins are key enzymes that catalyze the rate-limiting step in the fatty acid elongation pathway. The most recently discovered member of the Elovl family, Elovl8, has been proposed to be a fish-specific elongase with two gene paralogs described in teleosts. However, the biological functions of Elovl8 are still to be elucidated. In this study, we showed that in contrast to previous findings, elovl8 is not unique to teleosts, but displays a rather unique and ample phylogenetic distribution. For functional determination, we generated elovl8a (elovl8a−/−) and elovl8b (elovl8b−/−) zebrafish using CRISPR/Cas9 technology. Fatty acid composition in vivo and zebrafish liver cell experiments suggest that the substrate preference of Elovl8 overlapped with other existing Elovl enzymes. Zebrafish Elovl8a could elongate the polyunsaturated fatty acids (PUFAs) C18:2n-6 and C18:3n-3 to C20:2n-6 and C20:3n-3, respectively. Along with PUFA, zebrafish Elovl8b also showed the capacity to elongate C18:0 and C20:1. Gene expression quantification suggests that Elovl8a and Elovl8b may play a potentially important role in fatty acid biosynthesis. Overall, our results provide novel insights into the function of Elovl8a and Elovl8b, representing additional fatty acid elongases not previously described in chordates.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evolution and Functional Characteristics of the Novel elovl8 That Play Pivotal Roles in Fatty Acid Biosynthesis

Sun

Wang

Goh

et al. 2021

Genes

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“…Elovl are rate-limiting enzymes enabling the addition of two-carbon atoms to pre-existing fatty acid (FA) substrates (Guillou et al, 2010;Jakobsson et al, 2006). While a recent study has reported on the putative roles of a novel Elovl present, Elovl8, in PUFA elongation in teleosts (Li et al, 2020), robust evidence supports that mainly three members of the Elovl family, namely Elovl 2, 4 and 5, have been shown to participate in the biosynthesis of LC-PUFA in vertebrates (Jakobsson et al 2006;Guillou et al, 2010;Castro et al 2016). More specifically, Elovl5 exhibits high activity toward C16-20 PUFA substrates, while Elovl2 shows high activity toward C20-22 PUFA (Jakobsson et al, 2006;Monroig et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The fatty acid elongation genes elovl4a and elovl4b are present and functional in the genome of tambaqui (Colossoma macropomum)

Ferraz

Machado

Navarro

et al. 2020

Comparative Biochemistry and Physiology Part B: Biochemistry an

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“…The Elovl protein family contains several members [2][3][4], of which only a few have been demonstrated to have PUFA as substrates. Of these, Elovl2, Elovl4, and Elovl5, have well-established roles in the biosynthesis of LC-PUFA in vertebrates [2][3][4], while a novel Elovl8 has been more recently suggested to be also involved in PUFA elongation [5,6]. While Elovl2 and Elovl5 are primarily involved in elongation steps within the LC-PUFA biosynthesis pathway, Elovl4 catalyzes the synthesis of very long-chain (>C 24 ) PUFA (VLC-PUFA), which can have up to 36 or 38 carbons [1,7].…”

Section: Introductionmentioning

confidence: 99%

Molecular and Functional Characterization of Elovl4 Genes in Sparus aurata and Solea senegalensis Pointing to a Critical Role in Very Long-Chain (>C24) Fatty Acid Synthesis during Early Neural Development of Fish

Morais

Torres

Hontoria

et al. 2020

IJMS

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Very long-chain fatty acids (VLC-FA) play critical roles in neural tissues during the early development of vertebrates. However, studies on VLC-FA in fish are scarce. The biosynthesis of VLC-FA is mediated by elongation of very long-chain fatty acid 4 (Elovl4) proteins and, consequently, the complement and activity of these enzymes determines the capacity that a given species has for satisfying its physiological demands, in particular for the correct development of neurophysiological functions. The present study aimed to characterize and localize the expression of elovl4 genes from Sparus aurata and Solea senegalensis, as well as to determine the function of their encoded proteins. The results confirmed that both fish possess two distinct elovl4 genes, named elovl4a and elovl4b. Functional assays demonstrated that both Elovl4 isoforms had the capability to elongate long-chain (C20–24), both saturated (SFA) and polyunsaturated (PUFA), fatty acid precursors to VLC-FA. In spite of their overlapping activity, Elovl4a was more active in VLC-SFA elongation, while Elovl4b had a preponderant elongation activity towards n-3 PUFA substrates, particularly in S. aurata, being additionally the only isoform that is capable of elongating docosahexaenoic acid (DHA). A preferential expression of elovl4 genes was measured in neural tissues, being elovl4a and elovl4b mRNAs mostly found in brain and eyes, respectively.

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Genome wide identification and functional characterization of two LC-PUFA biosynthesis elongase (elovl8) genes in rabbitfish (Siganus canaliculatus)

Cited by 44 publications

References 48 publications

Evolution and Functional Characteristics of the Novel elovl8 That Play Pivotal Roles in Fatty Acid Biosynthesis

Evolution and Functional Characteristics of the Novel elovl8 That Play Pivotal Roles in Fatty Acid Biosynthesis

The fatty acid elongation genes elovl4a and elovl4b are present and functional in the genome of tambaqui (Colossoma macropomum)

Molecular and Functional Characterization of Elovl4 Genes in Sparus aurata and Solea senegalensis Pointing to a Critical Role in Very Long-Chain (>C24) Fatty Acid Synthesis during Early Neural Development of Fish

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