Genome-wide identification of osmotic stress response gene in Arabidopsis thaliana

Li, Yong; Zhu, Yanming; Liu, Yang; Shu, Yongjun; Meng, Fanjiang; Lu, Yanmin; Bai, Xi; Liu, Bei; Guo, Dianjing

doi:10.1016/j.ygeno.2008.08.011

Cited by 25 publications

(17 citation statements)

References 27 publications

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“…We have also found that the mutant plants grown under high illumination compensated for the absence of α-CA4 and for reduced NPQ by the increase in the contents of both PsbS protein and the violaxanthin cycle components, the latter accompanied by an increase in violaxanthin deepoxidase activity [13]. In addition, α-ca4 gene was found among the genes that change the expression level under osmotic stress [136]. The content of α-ca4 gene transcripts became an order of magnitude higher in conditions of drought stress (Rudenko et al, in preparation).…”

Section: Ca In Granal Thylakoid Membranesmentioning

confidence: 63%

Role of Plant Carbonic Anhydrases under Stress Conditions

Rudenko¹,

Borisova‐Mubarakshina²,

Ignatova³

et al. 2021

Plant Stress Physiology

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Carbonic anhydrases (CAs) are enzymes catalyzing the reversible hydration of carbon dioxide with the generation of protons and bicarbonate. The components of the reaction are involved in almost all metabolic processes in higher plants and algae, maintaining the balance of electrolytes and pH, gluconeogenesis, lipogenesis, ethylene synthesis, and others. The CAs may take part in transmitting signals to activate cascades of protective response genes. Our findings reveal significant changes in the content of carbonic anhydrase gene transcripts in response to changes in environmental conditions. Here we discuss the functions of CAs located in the plasma membrane, chloroplast envelope, chloroplast stroma, and in thylakoids in plant protection under stress conditions, such as high illumination, low and high concentration of carbon dioxide in the environment, drought, and salinity.

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Section: Ca In Granal Thylakoid Membranesmentioning

confidence: 63%

Role of Plant Carbonic Anhydrases under Stress Conditions

Rudenko¹,

Borisova‐Mubarakshina²,

Ignatova³

et al. 2021

Plant Stress Physiology

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“…RAHS-14 expressed more senescence-related genes, whereas we found that in Vagad, genes related to alcohol dehydrogenase and late embryogenesis protein 5 was expressed at a higher level (Additional files 5 and 15). Previous studies indicate that the accumulation of late embryogenesis abundant proteins and alcohol dehydrogenase (Adh) gene are correlated with stress tolerance [35,36]. The significant up-regulation of LEA and Adh genes in Vagad in drought stress suggests that these genes play an important role in conferring drought tolerance, whereas RAHS-14 tends toward the senescence during drought stress.…”

Section: Discussionmentioning

confidence: 99%

Genome wide expression profiling of two accession of G. herbaceum L. in response to drought

et al. 2012

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BackgroundGenome-wide gene expression profiling and detailed physiological investigation were used for understanding the molecular mechanism and physiological response of Gossypium herbaceum, which governs the adaptability of plants in drought conditions. Recently, microarray-based gene expression analysis is commonly used to decipher genes and genetic networks controlling the traits of interest. However, the results of such an analysis are often plagued due to a limited number of genes (probe sets) on microarrays. On the other hand, pyrosequencing of a transcriptome has the potential to detect rare as well as a large number of transcripts in the samples quantitatively. We used Affymetrix microarray as well as Roche's GS-FLX transcriptome sequencing for a comparative analysis of cotton transcriptome in leaf tissues under drought conditions.ResultsFourteen accessions of Gossypium herbaceum were subjected to mannitol stress for preliminary screening; two accessions, namely Vagad and RAHS-14, were selected as being the most tolerant and most sensitive to osmotic stress, respectively. Affymetrix cotton arrays containing 24,045 probe sets and Roche's GS-FLX transcriptome sequencing of leaf tissue were used to analyze the gene expression profiling of Vagad and RAHS-14 under drought conditions. The analysis of physiological measurements and gene expression profiling showed that Vagad has the inherent ability to sense drought at a much earlier stage and to respond to it in a much more efficient manner than does RAHS-14. Gene Ontology (GO) studies showed that the phenyl propanoid pathway, pigment biosynthesis, polyketide biosynthesis, and other secondary metabolite pathways were enriched in Vagad under control and drought conditions as compared with RAHS-14. Similarly, GO analysis of transcriptome sequencing showed that the GO terms responses to various abiotic stresses were significantly higher in Vagad. Among the classes of transcription factors (TFs) uniquely expressed in both accessions, RAHS-14 showed the expression of ERF and WRKY families. The unique expression of ERFs in response to drought conditions reveals that RAHS-14 responds to drought by inducing senescence. This was further supported by transcriptome analysis which revealed that RAHS-14 responds to drought by inducing many transcripts related to senescence and cell death.ConclusionThe comparative genome-wide gene expression profiling study of two accessions of G.herbaceum under drought stress deciphers the differential patterns of gene expression, including TFs and physiologically relevant processes. Our results indicate that drought tolerance observed in Vagad is not because of a single molecular reason but is rather due to several unique mechanisms which Vagad has developed as an adaptation strategy.

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“…Seven of them were confirmed to be connected to known biological processes in drought resistance, exhibiting the power of this method in analyzing plant microarray data for studying genotype-phenotype relationships. Similarly, Li et al (2008) reported a cis-regulatory element-based computational approach to genome-wide identification of genes putatively responding to various osmotic stresses in Arabidopsis . With this approach, the 500 top-scoring genes were selected, 91.3% of which were related to the stress response and ABA response in GO enrichment analysis.…”

Section: Physiological and Genetic Studies On Drought Tolerance In Brmentioning

confidence: 99%

Recent progress in drought and salt tolerance studies in <i>Brassica</i> crops

et al. 2014

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Water deficit imposed by either drought or salinity brings about severe growth retardation and yield loss of crops. Since Brassica crops are important contributors to total oilseed production, it is urgently needed to develop tolerant cultivars to ensure yields under such adverse conditions. There are various physiochemical mechanisms for dealing with drought and salinity in plants at different developmental stages. Accordingly, different indicators of tolerance to drought or salinity at the germination, seedling, flowering and mature stages have been developed and used for germplasm screening and selection in breeding practices. Classical genetic and modern genomic approaches coupled with precise phenotyping have boosted the unravelling of genes and metabolic pathways conferring drought or salt tolerance in crops. QTL mapping of drought and salt tolerance has provided several dozen target QTLs in Brassica and the closely related Arabidopsis. Many drought- or salt-tolerant genes have also been isolated, some of which have been confirmed to have great potential for genetic improvement of plant tolerance. It has been suggested that molecular breeding approaches, such as marker-assisted selection and gene transformation, that will enhance oil product security under a changing climate be integrated in the development of drought- and salt-tolerant Brassica crops.

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Genome-wide identification of osmotic stress response gene in Arabidopsis thaliana

Cited by 25 publications

References 27 publications

Role of Plant Carbonic Anhydrases under Stress Conditions

Role of Plant Carbonic Anhydrases under Stress Conditions

Genome wide expression profiling of two accession of G. herbaceum L. in response to drought

Recent progress in drought and salt tolerance studies in <i>Brassica</i> crops

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