Genome-wide identification of the restorer-of-fertility-like (RFL) gene family in Brassica napus and expression analysis in Shaan2A cytoplasmic male sterility

Ning, Luyun; Wang, Hao; Li, Dianrong; Li, Yonghong; Chen, Kang; Chao, Hongbo; Li, Huaixin; He, Jianjie; Li, Maoteng

doi:10.1186/s12864-020-07163-z

Cited by 8 publications

(10 citation statements)

References 52 publications

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“…The identification of the relationship between the maintainer and restorer in rapeseed is crucial for cultivating new varieties, which is directly related to transferring a sterile line or breeding a restorer [ 1 , 27 , 28 , 29 , 30 ]. In this study, the S and N molecular markers developed are derived from the indel differences in mitochondrial DNA between fertile cytoplasmic N and sterile cytoplasmic S, while the R and r molecular markers developed are derived from core SNP differences between the restorer gene R and the sterility gene r in the nucleus [ 12 , 31 ]. The combination of these molecular markers can rapidly and accurately identify the CMS lines, maintainer lines, restorer lines and hybrids of the pol and shaan CMS systems [ 2 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, the S and N molecular markers developed are derived from the indel differences in mitochondrial DNA between fertile cytoplasmic N and sterile cytoplasmic S, while the R and r molecular markers developed are derived from core SNP differences between the restorer gene R and the sterility gene r in the nucleus [ 12 , 31 ]. The combination of these molecular markers can rapidly and accurately identify the CMS lines, maintainer lines, restorer lines and hybrids of the pol and shaan CMS systems [ 2 , 31 ]. This can be applied to the identification of both the maintainer or restorer relationship in inbred lines, and the rapeseed purity of the cytoplasmic three-line rapeseed hybrids.…”

Section: Discussionmentioning

confidence: 99%

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A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus

Zhang

Song

et al. 2023

Plants

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Cytoplasmic male sterility (CMS) is the main mechanism employed to utilize the heterosis of Brassica napus. CMS three-line rapeseed hybrids have dramatically enhanced yield and brought about the global revolution of hybrid varieties, replacing conventional crop varieties. Over the last half century, China has led the development of hybrid Brassica napus varieties. Two sterile lines, polima (pol) and shaan 2A, were of particular importance for the establishment of three-line hybrid systems in rapeseed, which has opened up a new era of heterosis utilization. However, in current breeding practices, it takes up to three years to identify the restorer or maintainer relationship and the cytoplasmic type of any inbred material. This greatly affects the breeding speed of new varieties and inhibits the rapid development of the rapeseed industry. To address this problem, we developed a set of molecular markers for the identification of fertile cytoplasmic gene N and sterile cytoplasmic gene S, as well as for the fertile nucleus gene R and sterile nucleus gene r, based on differences in the gene sequences between the CMS line, maintainer line and restorer line of Brassica napus. Combining these markers can accurately identify the CMS line, maintainer and restorer of both the pol and shaan systems, as well as their hybrids. These markers can not only be used to identify of the maintainer and restorer relationship of inbred materials; they can also be used as general molecular markers to identify the CMS-type hybrid purity of pol and shaan systems.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus

Zhang

Song

et al. 2023

Plants

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“…Interestingly, there are many PPR genes in this region, including two restorer genes, Rfp (identified in Polima CMS; locus: 31.64–31.67 Mb) ( Liu et al, 2012 ) and Rfn (identified in nap CMS; locus: 31.79–31.81 Mb) ( Liu et al, 2017 ). Recent research has revealed that a highly dense cluster of restorer-of-fertility-like ( RFL ) genes exists in this region on chromosome A09 and on a collinear region on chromosome C8 in B. napus ( Ning et al, 2020 ). A similar PPR cluster is present in the collinear region of Arabidopsis chromosome A1.…”

Section: Discussionmentioning

confidence: 99%

Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

Yang

Nong

et al. 2021

Front. Plant Sci.

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Crosses that lead to heterosis have been widely used in the rapeseed (Brassica napus L.) industry. Cytoplasmic male sterility (CMS)/restorer-of-fertility (Rf) systems represent one of the most useful tools for rapeseed production. Several CMS types and their restorer lines have been identified in rapeseed, but there are few studies on the mechanisms underlying fertility restoration. Here, we performed morphological observation, map-based cloning, and transcriptomic analysis of the F2 population developed by crossing the CMS line WNJ01A with its restorer line Hui01. Paraffin-embedded sections showed that the sporogenous cell stage was the critical pollen degeneration period, with major sporogenous cells displaying loose and irregular arrangement in sterile anthers. Most mitochondrial electron transport chain (mtETC) complex genes were upregulated in fertile compared to sterile buds. Using bulked segregant analysis (BSA)-seq to analyze mixed DNA pools from sterile and fertile F2 buds, respectively, we identified a 6.25 Mb candidate interval where Rfw is located. Using map-based cloning experiments combined with bacterial artificial chromosome (BAC) clone sequencing, the candidate interval was reduced to 99.75 kb and two pentatricopeptide repeat (PPR) genes were found among 28 predicted genes in this interval. Transcriptome sequencing showed that there were 1679 DEGs (1023 upregulated and 656 downregulated) in fertile compared to sterile F2 buds. The upregulated differentially expressed genes (DEGs) were enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) lysine degradation pathway and phenylalanine metabolism, and the downregulated DEGs were enriched in cutin, suberine, and wax biosynthesis. Furthermore, 44 DEGs were involved in pollen and anther development, such as tapetum, microspores, and pollen wall development. All of them were upregulated except a few such as POE1 genes (which encode Pollen Ole e I allergen and extensin family proteins). There were 261 specifically expressed DEGs (9 and 252 in sterile and fertile buds, respectively). Regarding the fertile bud-specific upregulated DEGs, the ubiquitin–proteasome pathway was enriched. The top four hub genes in the protein–protein interaction network (BnaA09g56400D, BnaA10g18210D, BnaA10g18220D, and BnaC09g41740D) encode RAD23d proteins, which deliver ubiquitinated substrates to the 26S proteasome. These findings provide evidence on the pathways regulated by Rfw and improve our understanding of fertility restoration.

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“…Several important functional sites and binding sites of the fertility restorer gene Rfo and the sterility gene orf138 have been identified. Most restorer genes encode pentatricopeptide repeat (PPR) proteins, and all identified Rf-PPR genes evolved from a unique subset of the PPR gene family called the Rf-like or RFL gene family [ 176 , 177 ]. PPR proteins are characterized by tandem repeats of 35-amino-acid motifs, most of which are considered sequence-specific RNA-binding proteins, and they regulate mitochondrial and chloroplast gene expression through editing, splicing and cleavage [ 178 , 179 , 180 , 181 ].…”

Section: Mechanisms Of Ogura Cms Fertility Restorer Genes In Major Cr...mentioning

confidence: 99%

Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops

Ren

Chen

et al. 2022

IJMS

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Hybrid production using lines with cytoplasmic male sterility (CMS) has become an important way to utilize heterosis in vegetables. Ogura CMS, with the advantages of complete pollen abortion, ease of transfer and a progeny sterility rate reaching 100%, is widely used in cruciferous crop breeding. The mapping, cloning, mechanism and application of Ogura CMS and fertility restorer genes in Brassica napus, Brassica rapa, Brassica oleracea and other cruciferous crops are reviewed herein, and the existing problems and future research directions in the application of Ogura CMS are discussed.

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Genome-wide identification of the restorer-of-fertility-like (RFL) gene family in Brassica napus and expression analysis in Shaan2A cytoplasmic male sterility

Cited by 8 publications

References 52 publications

A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus

A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus

Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops

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