Genome-wide identification of the SWEET gene family in wheat

Gao, Yue; Wang, Zi Yuan; Kumar, Vinod; Xu, Xiao; Yuan, De Peng; Zhu, Xiao Jun; Li, Tianya; Jia, Baolei; Xuan, Yuan

doi:10.1016/j.gene.2017.11.044

Cited by 59 publications

(29 citation statements)

References 36 publications

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“…In this study, five pairs of JrSWEET genes were detected as tandem duplications. This finding agrees with other reports of SWEET gene duplications, including segmental, tandem, or whole genome duplications [32,33,50]. However, gene loss during expansion was also found in the J. regia genome, compared to the AtSWEET and OsSWEET genes.…”

Section: Discussionsupporting

confidence: 92%

“…Expansion and/or loss of SWEET genes have also occurred in other species, indicating possible functional variation in the evolution of this gene family. For example, the absence of SWEET10 in rice and wheat may suggest different roles of SWEET genes in dicot and monocot plants [15,32]. The expansion of SWEET genes in cucumber suggests possible functional differentiations in response to environmental conditions [51].…”

Section: Discussionmentioning

confidence: 99%

“…Host SWEET proteins are induced in many other systems such as the infection of A. thaliana by Pseudomonas syringae pv. tomato DC3000, and by the obligate biotrophic powdery mildew pathogen Golovinomyces cichoracearum [28], the infection of grapes with Botrytis cinerea [29], Medicago truncatula with Sinorhizobium meliloti [30], oilseed rape by Sclerotinia sclerotiorum [31], wheat by Puccinia [32], banana by Fusarium oxysporum f. sp. cubense TR4 [33], and cabbage by Plasmodiophora brassicae [34].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Profiling and Phylogenetic Analysis of the SWEET Sugar Transporter Gene Family in Walnut and Their Lack of Responsiveness to Xanthomonas arboricola pv. juglandis Infection

Jiang

Balan

Assis

et al. 2020

IJMS

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Following photosynthesis, sucrose is translocated to sink organs, where it provides the primary source of carbon and energy to sustain plant growth and development. Sugar transporters from the SWEET (sugar will eventually be exported transporter) family are rate-limiting factors that mediate sucrose transport across concentration gradients, sustain yields, and participate in reproductive development, plant senescence, stress responses, as well as support plant-pathogen interaction, the focus of this study. We identified 25 SWEET genes in the walnut genome and distinguished each by its individual gene structure and pattern of expression in different walnut tissues. Their chromosomal locations, cis-acting motifs within their 5 regulatory elements, and phylogenetic relationship patterns provided the first comprehensive analysis of the SWEET gene family of sugar transporters in walnut. This family is divided into four clades, the analysis of which suggests duplication and expansion of the SWEET gene family in Juglans regia. In addition, tissue-specific gene expression signatures suggest diverse possible functions for JrSWEET genes. Although these are commonly used by pathogens to harness sugar products from their plant hosts, little was known about their role during Xanthomonas arboricola pv. juglandis (Xaj) infection. We monitored the expression profiles of the JrSWEET genes in different tissues of "Chandler" walnuts when challenged with pathogen Xaj417 and concluded that SWEET-mediated sugar translocation from the host is not a trigger for walnut blight disease development. This may be directly related to the absence of type III secretion system-dependent transcription activator-like effectors (TALEs) in Xaj417, which suggests different strategies are employed by this pathogen to promote susceptibility to this major aboveground disease of walnuts.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Profiling and Phylogenetic Analysis of the SWEET Sugar Transporter Gene Family in Walnut and Their Lack of Responsiveness to Xanthomonas arboricola pv. juglandis Infection

Jiang

Balan

Assis

et al. 2020

IJMS

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“…For example, the expression level of TaSTP13 is increased during leaf rust infection of wheat (Savadi et al, 2017). Five SWEET family members are induced in wheat leaves infected by stem rust (Gao et al, 2018). Therefore, we speculate that these sugar transporters could participate in sugar distribution in the interaction between wheat and rust fungi.…”

Section: Pst-induced Tastp6 Upregulation Is Possibly Mediated By Abamentioning

confidence: 89%

ABA-Induced Sugar Transporter TaSTP6 Promotes Wheat Susceptibility to Stripe Rust

et al. 2019

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Biotrophic pathogens, such as wheat rust fungi, survive on nutrients derived from host cells. Sugar appears to be the major carbon source transferred from host cells to various fungal pathogens; however, the molecular mechanism by which host sugar transporters are manipulated by fungal pathogens for nutrient uptake is poorly understood. TaSTP6, a sugar transporter protein in wheat (Triticum aestivum), was previously shown to exhibit enhanced expression in leaves upon infection by Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust. In this study, we found that Pst infection caused increased accumulation of abscisic acid (ABA) and that application of exogenous ABA significantly enhanced TaSTP6 expression. Moreover, knockdown of TaSTP6 expression by barley stripe mosaic virus-induced gene silencing reduced wheat susceptibility to the Pst pathotype CYR31, suggesting that TaSTP6 expression upregulation contributes to Pst host sugar acquisition. Consistent with this, TaSTP6 overexpression in Arabidopsis (Arabidopsis thaliana) promoted plant susceptibility to powdery mildew and led to increased Glc accumulation in the leaves. Functional complementation assays in Saccharomyces cerevisiae showed that TaSTP6 has broad substrate specificity, indicating that TaSTP6 is an active sugar transporter. Subcellular localization analysis indicated that TaSTP6 localizes to the plasma membrane. Yeast two-hybrid and bimolecular fluorescence complementation experiments revealed that TaSTP6 undergoes oligomerization. Taken together, our results suggest that Pst stimulates ABA biosynthesis in host cells and thereby upregulates TaSTP6 expression, which increases sugar supply and promotes fungal infection.

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“…Thell] (2n = 4x = 28; AABB), which subsequently hybridized with Aegilops tauschii (D-genome donor) to form the current genome composition of bread wheat (2n = 6x = 42; AABBDD) [ 38 ]. These events have modified the number and/or levels of expression of different gene families, such as EXP expansins, SWEET sugar transporters, MAPK and MAPKK kinases, PHT1 phosphate transporters, among others, implicating a change in their biological role [ 39 , 40 , 41 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Transcriptional Regulation of Aquaporin Genes in Bread Wheat (Triticum aestivum L.) under Water Stress

Madrid-Espinoza

Brunel-Saldias

Guerra

et al. 2018

Genes

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Aquaporins (AQPs) are transmembrane proteins essential for controlling the flow of water and other molecules required for development and stress tolerance in plants, including important crop species such as wheat (Triticum aestivum). In this study, we utilized a genomic approach for analyzing the information about AQPs available in public databases to characterize their structure and function. Furthermore, we validated the expression of a suite of AQP genes, at the transcriptional level, including accessions with contrasting responses to drought, different organs and water stress levels. We found 65 new AQP genes, from which 60% are copies expanded by polyploidization. Sequence analysis of the AQP genes showed that the purifying selection pressure acted on duplicate genes, which was related to a high conservation of the functions. This situation contrasted with the expression patterns observed for different organs, developmental stages or genotypes under water deficit conditions, which indicated functional divergence at transcription. Expression analyses on contrasting genotypes showed high gene transcription from Tonoplast Intrinsic Protein 1 (TIP1) and 2 (TIP2), and Plasma Membrane Intrinsic Protein 1 (PIP1) and 2 (PIP2) subfamilies in roots and from TIP1 and PIP1 subfamilies in leaves. Interestingly, during severe drought stress, 4 TIP genes analyzed in leaves of the tolerant accession reached up to 15-fold the level observed at the susceptible genotype, suggesting a positive relationship with drought tolerance. The obtained results extend our understanding of the structure and function of AQPs, particularly under water stress conditions.

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Genome-wide identification of the SWEET gene family in wheat

Cited by 59 publications

References 36 publications

Genome-Wide Profiling and Phylogenetic Analysis of the SWEET Sugar Transporter Gene Family in Walnut and Their Lack of Responsiveness to Xanthomonas arboricola pv. juglandis Infection

Genome-Wide Profiling and Phylogenetic Analysis of the SWEET Sugar Transporter Gene Family in Walnut and Their Lack of Responsiveness to Xanthomonas arboricola pv. juglandis Infection

ABA-Induced Sugar Transporter TaSTP6 Promotes Wheat Susceptibility to Stripe Rust

Genome-Wide Identification and Transcriptional Regulation of Aquaporin Genes in Bread Wheat (Triticum aestivum L.) under Water Stress

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