Genome-wide transcriptomic analysis of the effects of sub-ambient atmospheric oxygen and elevated atmospheric carbon dioxide levels on gametophytes of the moss, Physcomitrella patens

Shinde, Suhas; Behpouri, A.; McElwain, Jennifer C.; Ng, Carl

doi:10.1093/jxb/erv197

Cited by 15 publications

(12 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Out of the unique metabolites, glycine, proline, and ABA showed significant increases at 5 mpi in MCs, while ethylene glycol, myricetin, niacin, suberylglycine, succinic semialdehyde, and uric acid showed significant decreases at 60 mpi in GCs ( S4 Fig ). In addition, the number of increased metabolites outnumbered the decreased in the two cell-types ( S5 Fig ), which corroborated the recent transcriptomic findings [ 43 ]. Furthermore, there more number of significantly changed metabolites in GCs than in MCs ( S2 Fig ).…”

Section: Resultssupporting

confidence: 90%

“…MC-specific increases of phenylpropanoids as compared to GCs indicate that MCs may be the biosynthetic hubs for plant foliar defense systems as a result of phenylpropanoid accumulation [ 65 , 66 ]. Transcripts involved in stress-responsive functions are known to be highly induced upon elevated CO 2 treatment [ 43 ]. Flavonoids biosynthesis is downstream of phenylpropanoid metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…Although less severe in C3 plants, increased oxidative stress was found in plants grown under elevated CO 2 [ 73 , 74 ]. In addition, up-regulation of genes encoding enzymes involved in oxidative signaling and ROS scavenging such as glutathione-S-transferase, peroxidases, catalases, cysteine and thioredoxin-pathway related genes was reported [ 43 ]. Elevated CO 2 can enhance maintenance of the redox potential due to an elevated rate of CO 2 assimilation and low photorespiration [ 75 ].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolomic Responses of Guard Cells and Mesophyll Cells to Bicarbonate

et al. 2015

View full text Add to dashboard Cite

Anthropogenic CO2 presently at 400 ppm is expected to reach 550 ppm in 2050, an increment expected to affect plant growth and productivity. Paired stomatal guard cells (GCs) are the gate-way for water, CO2, and pathogen, while mesophyll cells (MCs) represent the bulk cell-type of green leaves mainly for photosynthesis. We used the two different cell types, i.e., GCs and MCs from canola (Brassica napus) to profile metabolomic changes upon increased CO2 through supplementation with bicarbonate (HCO3 -). Two metabolomics platforms enabled quantification of 268 metabolites in a time-course study to reveal short-term responses. The HCO3 - responsive metabolomes of the cell types differed in their responsiveness. The MCs demonstrated increased amino acids, phenylpropanoids, redox metabolites, auxins and cytokinins, all of which were decreased in GCs in response to HCO3 -. In addition, the GCs showed differential increases of primary C-metabolites, N-metabolites (e.g., purines and amino acids), and defense-responsive pathways (e.g., alkaloids, phenolics, and flavonoids) as compared to the MCs, indicating differential C/N homeostasis in the cell-types. The metabolomics results provide insights into plant responses and crop productivity under future climatic changes where elevated CO2 conditions are to take center-stage.

show abstract

Section: Resultssupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolomic Responses of Guard Cells and Mesophyll Cells to Bicarbonate

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Therefore, collection and comparison of the transcriptome of different tissue types, and developmental stages is the best strategy to investigate plant response regulation and to identify genes involved in stress tolerance mechanisms. Thus, understanding the transcriptome of different tissue types or developmental stages would lead to a deeper understanding of corresponding phenotypic change [52]. Many tools and techniques are available for the evaluation of the transcriptomic data to get expression profiling for the gene-by-gene as well as collectively for many genes at a time [53].…”

Section: Advances In Transcriptomicsmentioning

confidence: 99%

Advances in Omics Approaches for Abiotic Stress Tolerance in Tomato

Chaudhary

Khatri

Singla

et al. 2019

Biology

View full text Add to dashboard Cite

Tomato, one of the most important crops worldwide, has a high demand in the fresh fruit market and processed food industries. Despite having considerably high productivity, continuous supply as per the market demand is hard to achieve, mostly because of periodic losses occurring due to biotic as well as abiotic stresses. Although tomato is a temperate crop, it is grown in almost all the climatic zones because of widespread demand, which makes it challenge to adapt in diverse conditions. Development of tomato cultivars with enhanced abiotic stress tolerance is one of the most sustainable approaches for its successful production. In this regard, efforts are being made to understand the stress tolerance mechanism, gene discovery, and interaction of genetic and environmental factors. Several omics approaches, tools, and resources have already been developed for tomato growing. Modern sequencing technologies have greatly accelerated genomics and transcriptomics studies in tomato. These advancements facilitate Quantitative trait loci (QTL) mapping, genome-wide association studies (GWAS), and genomic selection (GS). However, limited efforts have been made in other omics branches like proteomics, metabolomics, and ionomics. Extensive cataloging of omics resources made here has highlighted the need for integration of omics approaches for efficient utilization of resources and a better understanding of the molecular mechanism. The information provided here will be helpful to understand the plant responses and the genetic regulatory networks involved in abiotic stress tolerance and efficient utilization of omics resources for tomato crop improvement.

show abstract

“…Many studies have used DNA microarrays to infer how organisms respond to different environments (Gracey and Cossins, 2003;Cossins et al, 2006;Gracey, 2007;Evans and Hofmann, 2012), such as temperature Yang et al, 2010;Long et al, 2012;Aguado-Urda et al, 2013;Logan and Buckley, 2015), osmolality (Posas et al, 2000;Evans and Somero, 2008;Melamed et al, 2008;Halbeisen andGerber, 2009), oxygen (Ton et al, 2003;Garnczarska, 2006;Swiderek et al, 2008;Otsuka et al, 2010;Gracey et al, 2011;Shinde et al, 2015) and pH (Leaphart et al, 2006;Serrano et al, 2006;Worden et al, 2009;Evans et al, 2013). Detection of genes with expression changes in response to environmental change helps to predict the fragility, resistance, and adaptability of an organism, tissue, or cell in the environment.…”

Section: Evaluation For Environmental Effectsmentioning

confidence: 99%