Genomic organization and expression analyses of putrescine pathway genes in soybean

Eller, Michele H.; Warner, April L.; Knap, Halina T.

doi:10.1016/j.plaphy.2006.01.006

Cited by 22 publications

(15 citation statements)

References 44 publications

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“…DAO, which is localized to the plant cell wall, facilitates Put catabolism and is important for cross-linking reactions under stress conditions (Eller et al, 2006). Exogenous Spd increased DAO activity due to the concomitant decrease in free Put content.…”

Section: Discussionmentioning

confidence: 99%

The Variation Tendency of Polyamines Forms and Components of Polyamine Metabolism in Zoysiagrass (Zoysia japonica Steud.) to Salt Stress with Exogenous Spermidine Application

Cui

Zhang

et al. 2017

Front. Physiol.

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To understand dynamic changes in polyamines (PAs) forms and components of polyamine metabolism in zoysiagrass (Zoysia japonica Steud.) response to salt stress with exogenous spermidine (Spd) application, two Chinese zoysia cultivars, z081 and z057, were exposed to sodium chloride stress for 2, 4, 6, and 8 days. The z057 cultivar possesses higher salinity tolerance than the z081 cultivar. Salt stress decreased the zoysiagrass fresh weight (FW) and increased free Spd and spermine (Spm) levels and soluble and insoluble putrescine (Put), Spd and Spm levels in both cultivars. Moreover, salt stress enhanced the activities of arginine decarboxylase (ADC), ornithine decarboxylase (ODC), S-adenosylmethionine decarboxylase (SAMDC), and diamine oxidase (DAO). Exogenous Spd increased PA metabolism and ADC, SAMDC, and DAO activities and decreased free Put levels under salt stress conditions in both cultivars. In addition, structural equation modeling (SEM) showed that ODC, SAMDC, and DAO contributed to PA metabolism, and endogenous Spd levels also contributed to endogenous Spm levels. Free PAs may be the primary factor influencing the variation of other PA forms. SEM also indicated that ADC and polyamine oxidase (PAO) play a limited role in enhancing zoysia salt tolerance via PA metabolism under salt stress.

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Section: Discussionmentioning

confidence: 99%

The Variation Tendency of Polyamines Forms and Components of Polyamine Metabolism in Zoysiagrass (Zoysia japonica Steud.) to Salt Stress with Exogenous Spermidine Application

Cui

Zhang

et al. 2017

Front. Physiol.

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“…PAs are catabolized into ammonia and H 2 O 2 by DAO and PAO (Figure 1 ). These enzymes are localized in the plant cell wall, and hydrogen peroxide resulting from Put catabolism may be important in cross-linking reactions under both normal and stress conditions (Eller et al, 2006 ). In both cultivars, we observed a possible connection between the PA content and degradation enzyme with different exogenous Spd concentrations.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Exogenous Spermidine Concentration on Polyamine Metabolism and Salt Tolerance in Zoysiagrass (Zoysia japonica Steud) Subjected to Short-Term Salinity Stress

Han

Zhang

2016

Front. Plant Sci.

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Salt stress, particularly short-term salt stress, is among the most serious abiotic factors limiting plant survival and growth in China. It has been established that exogenous spermidine (Spd) stimulates plant tolerance to salt stress. The present study utilized two zoysiagrass cultivars commonly grown in China that exhibit either sensitive (cv. Z081) or tolerant (cv. Z057) adaptation capacity to salt stress. The two cultivars were subjected to 200 mM salt stress and treated with different exogenous Spd concentrations for 8 days. Polyamine [diamine putrescine (Put), tetraamine spermine (Spm), and Spd], H2O2 and malondialdehyde (MDA) contents and polyamine metabolic (ADC, ODC, SAMDC, PAO, and DAO) and antioxidant (superoxide dismutase, catalase, and peroxidase) enzyme activities were measured. The results showed that salt stress induced increases in Spd and Spm contents and ornithine decarboxylase (ODC), S-adenosylmethionine decarboxylase (SAMDC), and diamine oxidase (DAO) activities in both cultivars. Exogenous Spd application did not alter polyamine contents via regulation of polyamine-degrading enzymes, and an increase in polyamine biosynthetic enzyme levels was observed during the experiment. Increasing the concentration of exogenous Spd resulted in a tendency of the Spd and Spm contents and ODC, SAMDC, DAO, and antioxidant enzyme activities to first increase and then decrease in both cultivars. H2O2 and MDA levels significantly decreased in both cultivars treated with Spd. Additionally, in both cultivars, positive correlations between polyamine biosynthetic enzymes (ADC, SAMDC), DAO, and antioxidant enzymes (SOD, POD, CAT), but negative correlations with H2O2 and MDA levels, and the Spd + Spm content were observed with an increase in the concentration of exogenous Spd.

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“…Several ADC cDNA clones have been isolated and characterised from various species. Whereas in some plants a single gene encodes ADC (Kakar and Sawhney 2002;Delis et al 2005;Eller et al 2006), Arabidopsis has two copies of the ADC gene and duplication has also been found in 12 species of the Brassicaceae family (Galloway et al 1998;Mo and Pua 2002). Akiyama and Jin (2007) cloned the first OsADC1 cDNA (AY604047) and indicated that a putative OsADC2 sequence was present in the rice genome.…”

Section: Discussionmentioning

confidence: 99%

Spermine facilitates recovery from drought but does not confer drought tolerance in transgenic rice plants expressing Datura stramonium S-adenosylmethionine decarboxylase

et al. 2009

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Polyamines are known to play important roles in plant stress tolerance but it has been difficult to determine precise functions for each type of polyamine and their interrelationships. To dissect the roles of putrescine from the higher polyamines spermidine and spermine, we generated transgenic rice plants constitutively expressing a heterologous S-adenosylmethionine decarboxylase (SAMDC) gene from Datura stramonium so that spermidine and spermine levels could be investigated while maintaining a constant putrescine pool. Whereas transgenic plants expressing arginine decarboxylase (ADC) produced higher levels of putrescine, spermidine and spermine, and were protected from drought stress, transgenic plants expressing SAMDC produced normal levels of putrescine and showed drought symptoms typical of wild type plants under stress, but the transgenic plants showed a much more robust recovery on return to normal conditions (90% full recovery compared to 25% partial recovery for wild type plants). At the molecular level, both wild type and transgenic plants showed transient reductions in the levels of endogenous ADC1 and SAMDC mRNA, but only wild type plants showed a spike in putrescine levels under stress. In transgenic plants, there was no spike in putrescine but a smooth increase in spermine levels at the expense of spermidine. These results confirm and extend the threshold model for polyamine activity in drought stress, and attribute individual roles to putrescine, spermidine and spermine.

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Genomic organization and expression analyses of putrescine pathway genes in soybean

Cited by 22 publications

References 44 publications

The Variation Tendency of Polyamines Forms and Components of Polyamine Metabolism in Zoysiagrass (Zoysia japonica Steud.) to Salt Stress with Exogenous Spermidine Application

The Variation Tendency of Polyamines Forms and Components of Polyamine Metabolism in Zoysiagrass (Zoysia japonica Steud.) to Salt Stress with Exogenous Spermidine Application

The Effect of Exogenous Spermidine Concentration on Polyamine Metabolism and Salt Tolerance in Zoysiagrass (Zoysia japonica Steud) Subjected to Short-Term Salinity Stress

Spermine facilitates recovery from drought but does not confer drought tolerance in transgenic rice plants expressing Datura stramonium S-adenosylmethionine decarboxylase

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