Genomic reorganization between two sibling yeast species,Saccharomyces bayanus andSaccharomyces cerevisiae

Ryu, Seung-Lim; Murooka, Yoshikatsu; Kaneko, Yoshinobu

doi:10.1002/(sici)1097-0061(19960630)12:8<757::aid-yea970>3.0.co;2-h

Cited by 47 publications

(25 citation statements)

References 19 publications

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“…However, the order and sizes of the homologous chromosomes can vary in the six sibling species. Earlier Southern hybridization experiments using different cloned genes of S. cerevisiae clearly demonstrated the identity of the karyotypes of S. cerevisiae and S. paradoxus and the species specificity of the S. bayanus karyotype : at least four homologous chromosomes, II, IV, VIII and XV, are of different sizes ( Naumov et al, 1992( Naumov et al, b, 1994Ryu et al, 1996). The sizes of some homologous chromo- ( Naumov et al, 1992b) Table 1 have been used in karyotypic analysis in the past ( Naumov et al, 1992b ;Louis et al, 1994).…”

Section: Molecular Karyotypingmentioning

confidence: 99%

Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae.

Наумов¹,

James²,

Наумова³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

284

245

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Section: Molecular Karyotypingmentioning

confidence: 99%

Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae.

Наумов¹,

James²,

Наумова³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

284

245

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“…uvarum, while in S. bayanus var. bayanus only two reciprocal translocations are documented (Naumov et al, 1994a;Ryu et al, 1996Ryu et al, , 1998. For Southern hybridization we used four cloned genes located on S. cerevisiae chromosomes that are rearranged in S. bayanus var.…”

Section: Natural Hybrids Of S Cerevisiae × S Bayanus and S Cerevismentioning

confidence: 99%

Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae

Наумова

Наумов

Masneuf‐Pomarède

et al. 2005

Yeast

121

101

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The genomic constitution of different S. bayanus strains and natural interspecific Saccharomyces hybrids has been studied by genetic and molecular methods. Unlike S. bayanus var. uvarum, some S. bayanus var. bayanus strains (the type culture CBS 380, CBS 378, CBS 425, CBS 1548) harbour a number of S. cerevisiae subtelomeric sequences: Y , pEL50, SUC, RTM and MAL. The two varieties, having 86-100% nDNA-nDNA reassociation, are partly genetically isolated from one another but completely isolated from S. cerevisiae. Genetic and molecular data support the maintaining of var. bayanus and var. uvarum strains in the species S. bayanus. Using Southern hybridization with species-specific molecular markers, RFLP of the MET2 gene and flow cytometry analysis, we showed that the non-S. cerevisiae parents are different in lager brewing yeasts and in wine hybrid strains. Our results suggest that S. pastorianus is a hybrid between S. cerevisiae and S. bayanus var. bayanus, while S. bayanus var. uvarum contributed to the formation of the wine hybrids S6U and CID1. According to the partial sequence of ACT1 gene and flow cytometry analysis, strain CID1 is a triple hybrid between S. cerevisiae, S. kudriavzevii and S. bayanus var. uvarum.

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“…There are also several translocations among S. cerevisiae, S. bayanus, and S. paradoxus. Ryu et al (1996) have constructed hybrids between several of these species and find sterility in all combinations tested. They ar- There are also reports of another interesting feature of interspecific hybrids.…”

Section: Interspecific Hybridsmentioning

confidence: 99%

Evolution and Variation of the Yeast (Saccharomyces) Genome

2000

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In this review we describe the role of the yeast Saccharomyces in the development of human societies including the use of this organism in the making of wine, bread, beer, and distilled beverages. We also discuss the tremendous diversity of yeast found in natural (i.e., noninoculated) wine fermentations and the scientific uses of yeast over the past 60 years. In conclusion, we present ideas on the model of "genome renewal" and the use of this model to explain the mode by which yeast has evolved and how diversity can be generated. Genetic and Molecular Studies of the Yeast GenomeIn this discussion the term yeast is used rather loosely because there are hundreds of species of yeast. However, we refer to only a few species in this article, Saccharomyces cerevisiae, Saccharomyces carlsbergensis, Saccharomyces bayanus, and Saccharomyces paradoxus, which are used in the making of wine, bread, beer, and distilled beverages. Taxonomically, yeast is defined as a single-cell eukaryotic microorganism that usually divides by budding.Although humans have used yeast for centuries (see Box 1), the yeast S. cerevisiae has only been studied at the genetic level since the mid 1930s with the pioneering work of Winge (1935). He used as his starting material, strains of Saccharomyces that had been isolated many years before by Hansen (1891). Lindegren, also one of the pioneers of yeast genetics, used a strain, EM93, that had been isolated by Emil Mrak (Mortimer and Johnston 1986). This strain was found on rotting figs in Merced, California, in 1938, and turned out to be heterothallic (i.e., having two types of sterile haploid individuals which are fertile when together). Other strains found on the same figs were homothallic (one haploid phase that produces gametes capable of fusing to create a zygote).The strains that Winge studied were nearly all homothallic, which required particular skills in the making of crosses. Lindegren was fortunate in being given a heterothallic strain, constructing crosses was very easy, and eventually this led to the development of modern yeast genetics. However, most of the studies of yeast genetics and, subsequently, yeast molecular biology, were carried out on a very few strains, most of which were derived from Lindegren's original strain EM93. Strain S288C, used to sequence the yeast genome, was constructed in the early 1950s (Mortimer et al. 1957;Mortimer and Johnston 1986). It has been estimated that Ն85% of the genome of S288C comes from EM93 (Mortimer and Johnston 1986). It seems likely that this original diploid strain was also a wine yeast (∼10% of wine yeast are heterothallic) and was carried to the rotting figs by insects; the yeast S. cerevisae does not travel independently through the air.Genetic research on the brewing yeasts shows that most have extremely low spore viabilities. Other research suggests that some of them may be polyploid or aneuploid (Johnston 1990). These characteristics would contribute to strain stability and this property may have been inadvertently selected by the brew...

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Genomic reorganization between two sibling yeast species,Saccharomyces bayanus andSaccharomyces cerevisiae

Cited by 47 publications

References 19 publications

Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae.

Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae.

Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae

Evolution and Variation of the Yeast (Saccharomyces) Genome

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