Genotoxic assessment in tobacco farmers at different crop times

Silva, Fernanda R. Da; Kvitko, Kátia; Rohr, Paula; Abreu, Marina B.; Thiesen, Flávia Valladão; Silva, Juliana da

doi:10.1016/j.scitotenv.2014.05.018

Cited by 36 publications

(21 citation statements)

References 48 publications

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“…A study from our group observed shortening telomeres in individuals occupationally chronically exposed to low doses of pesticides at tobacco farms [33], in addition to various kinds of DNA damages already found in those individuals [81][82][83][84], corroborated by several other studies [85,86]. Pesticides are known for inducing oxidative stress [33,82,87,88], and although not all action mechanism of these chemicals are clear, induction of oxidative stress and of ROS seems to be involved.…”

Section: Occupational and Environmental Exposuressupporting

confidence: 63%

“…Pesticides are known for inducing oxidative stress [33,82,87,88], and although not all action mechanism of these chemicals are clear, induction of oxidative stress and of ROS seems to be involved. Senescent cells present 30% more modified guanines within their DNA and fourfold more free 8-oxo-dG basis, contributing to telomeric loss through oxidative stress [89].…”

Section: Occupational and Environmental Exposuresmentioning

confidence: 99%

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Telomere Length and Its Relation to Human Health

Kahl¹,

Silva²

2016

Telomere - A Complex End of a Chromosome

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Telomeres are complex nucleotide sequences that cap the end of chromosomes from degradation, unwanted recombination-fusion, inappropriate activation of DNA damage response and play a critical role in cell division and chromosome stability. There is growing evidence that telomere stability can be affected by occupational and environmental exposure, as some of these factors have been correlated with increase in inflammation, oxidative stress, DNA damage, chromosome aberration, and epigenetic alterations. Both extremely short and long telomeres have been associated with neurodegenerative, cardiovascular diseases (CVD) and cancer risk. Occupational and environmental exposure to several synthetic and natural chemicals has been found to be associated with changes in telomere length, although the molecular mechanism is not fully understood. Telomeric DNA is relatively less capable of repair, resulting in accelerated shortening during the cell cycle and replicative senescence. It is recognized that diet plays an important role in telomere maintenance. Prevention of exposure to environmental and occupational hazards as well as psychological stressors can reduce the risk of telomere instability. This review provides a broad evaluation of the associated mechanism between human health and environmental and occupational exposure with telomere length, including recent findings and future perspectives.

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Section: Occupational and Environmental Exposuressupporting

confidence: 63%

Section: Occupational and Environmental Exposuresmentioning

confidence: 99%

Telomere Length and Its Relation to Human Health

Kahl¹,

Silva²

2016

Telomere - A Complex End of a Chromosome

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“…Cyazofamid shows higher activity in resistant Phytophthora infestans than metalaxyl (Mitani et al, ). However, the presence of metalaxyl and cyazofamid residues on tobacco could be of concern (Da Silva et al, ).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of residues of metalaxyl, cyazofamid and a cyazofamid metabolite in tobacco leaves and soil by liquid chromatography with tandem mass spectrometry

Sun

et al. 2018

Biomedical Chromatography

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A simple method was developed and validated for the simultaneous determination of metalaxyl, cyazofamid and the cyazofamid metabolite 4-chloro-5-p-tolylimidazole-2-carbonitrile (CCIM) by liquid chromatography with tandem mass spectrometry. The three target compounds were extracted from tobacco and soil with acetonitrile containing 0.1% acetic acid, and the extracts were purified using octadecylsilane. The proposed method showed satisfactory linearity (R ≥ 0.9985) for the target compounds. The limits of detection for metalaxyl, cyazofamid and CCIM were 0.006, 0.06 and 0.06 mg/kg in soil and green tobacco leaves and 0.03, 0.3 and 0.3 mg/kg in cured tobacco leaves, respectively. The limits of quantification for metalaxyl, cyazofamid and CCIM were 0.02, 0.2 and 0.2 mg/kg in soil and green tobacco leaves and 0.1, 1 and 1 mg/kg in cured tobacco leaves, respectively. The average recoveries from soil and tobacco were 72.91-98.40% for metalaxyl, 76.73-105.80% for cyazofamid and 74.48-106.45% for CCIM. The relative standard deviation range was 1.23-6.99%. The developed method was successfully applied to analysis of residues of metalaxyl, cyazofamid and CCIM in real soil and tobacco samples. The results indicated that the established method could meet the requirement for the analysis of trace amounts of all three analytes in soil and tobacco.

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“…Tobacco farming is an agricultural activity requiring special attention for its occupational health risks because this crop requires huge amounts of pesticides to prevent disease and pest infestation [Agostinetto et al, ]. Although it is very economically and socially important to Brazil [IBGE, ], tobacco farming poses several hazards to those who cultivate and harvest the plants [Da Silva et al, ,,]. These farmers are routinely occupationally exposed to pesticides including organophosphorus (OP) and pyrethroids (PYR), which are both highly toxic and are among the most used [Agostinetto et al, ].…”

Section: Introductionmentioning

confidence: 99%

“…A recent study showed that 17% of 2,400 tobacco farmers use pesticides 10 or more days a month [Faria et al, ]. In addition, during the harvest time farmers are exposed to nicotine, a natural pesticide that exerts different cell damaging effects [Da Silva et al, ,,].…”

Section: Introductionmentioning

confidence: 99%

Telomere measurement in individuals occupationally exposed to pesticide mixtures in tobacco fields

Kahl

Simon

Salvador

et al. 2015

Environ and Mol Mutagen

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Occupational exposure to pesticides in tobacco fields causes genetic damage in farmers. The aim of this study was to analyze tobacco farmers chronically exposed to low doses of pesticides and nicotine (present in the tobacco leaves) in relation to absolute telomere length (aTL), and explore the influence of lifestyle characteristics, oxidative stress, and inorganic element levels. DNA was isolated from peripheral blood samples from agricultural workers and non-exposed individuals, and aTL was measured by quantitative real time polymerase chain reaction (qPCR) analysis. Oxidative stress (thiobarbituric acid reactive substances [TBARS], which measures oxidative damage to lipids; and toxic equivalent antioxidant capacity [TEAC], which measures total equivalent antioxidant capacity) was evaluated in serum, and inorganic element content was analyzed in whole blood through particle-induced X-ray emission technique. It was found that exposure to pesticides and tobacco smoking had significant effects on aTL. Individuals occupationally exposed to complex mixtures of pesticides in tobacco fields and individuals who smoked had decreased aTL compared with the non-exposed group. TBARS and TEAC were significantly elevated in the exposed group. There were no significant differences in inorganic elements. There was no evidence of an influence of age, gender, consumption of alcoholic beverages, or intake of fruits and vegetables on aTL within the groups. In addition, years of work in the tobacco field in the exposed group did not influence any of the variables analyzed. Although further studies were needed, these results suggested differences in telomere maintenance in tobacco farmers compared with the control group, indicating that telomere length may be a good biomarker of occupational exposure.

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Genotoxic assessment in tobacco farmers at different crop times

Cited by 36 publications

References 48 publications

Telomere Length and Its Relation to Human Health

Telomere Length and Its Relation to Human Health

Simultaneous determination of residues of metalaxyl, cyazofamid and a cyazofamid metabolite in tobacco leaves and soil by liquid chromatography with tandem mass spectrometry

Telomere measurement in individuals occupationally exposed to pesticide mixtures in tobacco fields

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