Genotoxic effects of catmint (<i>Nepeta meyeri</i>Benth.) essential oils on some weed and crop plants

Kekeç, Güzin; Mutlu, Salih; Alpsoy, Lokman; Sakcali, Mehmet Serdal; Atıcı, Ökkeş

doi:10.1177/0748233712440135

Cited by 28 publications

(10 citation statements)

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“…Because, similar to other stresses, reactive oxygen species (ROS) such as superoxide anion (O − 2 ), CONTACT Salih Mutlu salihmutlu@yahoo.com H 2 O 2 and hydroxyl radicals ( · OH) are produced during response to cold stress (Mutlu et al 2009a(Mutlu et al , 2009bHu et al 2010). Toxic levels of ROS can impair membrane permeability (Dias et al 2011) by inducing lipid peroxidation (Mutlu et al 2011;Esim et al 2014), cause damage to DNA and proteins (Bertrand et al 2011;Kekec et al 2013) and ultimately lead to programmed cell death. These symptoms during cold stress have been evaluated as oxidative damage (Wang et al 2009a).…”

Section: Introductionmentioning

confidence: 99%

Exogenous salicylic acid alleviates cold damage by regulating antioxidative system in two barley (Hordeum vulgareL.) cultivars

Mutlu

Atıcı

Nalbantoğlu

et al. 2016

Frontiers in Life Science

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In this work, the time-dependent effect of exogenous salicylic acid (SA) applied before cold stress was investigated on the regulation of antioxidative respond mechanisms in two barley (Hordeum vulgare L.) cultivars (Akhisar and Tokak) differed in cold tolerance. SA (0.1 mM) was applied to 7-days old barley seedlings growing under control conditions (20/18°C). After this application, the seedlings were transferred to cold conditions (7/5°C) at different times (7, 14, 21 and 28 days) for 3 days. Then, the contents of malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ) and the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) were determined in the leaves (control, cold and SA + cold) from the seedlings harvested at 10, 17, 24 and 31 days. Moreover, the effect of cold stress was evaluated on the endogenous SA level at the both cultivars by comparing to control plants. The MDA content increased in the cold treatment while it decreased in the SA treatment at all the days studied at the both cultivars. The SA + cold treatment could have a variable effect on H 2 O 2 content at the tolerant barley (Tokak) while decreased its content at the sensitive cultivar (Akhisar). The SA + cold treatment could increase the activities of POX and SOD at both cultivars, but it increased the CAT activity at the tolerant cultivar while decreased at the sensitive cultivar. In addition, the content of endogenous SA was decreased by cold stress at all the days studied at the barley cultivars as compared to control plants. The results show that the SA treatment could be effective on the regulation of the parameters studied at cold conditions until 31 days after SA application, and the importance of exogenous SA treatment before cold stress. It was concluded that exogenous and endogenous SA could play an ameliorating role on cold tolerance by regulating reactive oxygen species and the activities of antioxidative enzymes in both cold-sensitive and cold-tolerant cultivars of barley. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Exogenous salicylic acid alleviates cold damage by regulating antioxidative system in two barley (Hordeum vulgareL.) cultivars

Mutlu

Atıcı

Nalbantoğlu

et al. 2016

Frontiers in Life Science

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“…A comparison of the germination percentage and random amplified polymorphic deoxyribonucleic acid (RAPD) of Brassica napus and Zea mays treated with essential oil of N. meyeri showed strong inhibitory effects on germination. A change in RAPD profiles such as differing band intensity, loss of bands, and the appearance of new bands compared with the control group, indicating a genotoxic effect of N. meyeri on weed and crop plants, has been observed (Kekeç, Mutlu, Alpsoy, Sakçali, & Atici, ). Bozari, Agar, Aksakal, Erturk, and Yanmis () demonstrated genotoxic effects of the essential oil obtained from Nepeta nuda L. containing mainly nepetalactone, germacrene, and elemol.…”

Section: Biological Activity Studies On Nepeta L Genusmentioning

confidence: 99%

“…kopetdaghensis , and N. gloeocephala , displayed cytotoxic effect (Afshar et al, ; Shakeri et al, , ). N. meyeri and N. nuda caused genotoxic effects (Bozari et al, ; Kekeç et al, ). N. gloeocephala was demonstrated to possess apoptotic potential (Dehghany Ashkezary et al, ).…”

Section: Biological Activity Studies On Nepeta L Genusmentioning

confidence: 99%

Pharmacological and chemical features of Nepeta L. genus: Its importance as a therapeutic agent

Süntar

Nabavi

Barreca

et al. 2017

Phytotherapy Research

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Medicinal plants have always had great value for the human population due to their valuable constituents and potential bioactivities. The objective of this review is to present an updated overview of an important medicinal plant genus Nepeta L., from the family Lamiaceae, revealing its traditional utilization, biological activity, phytoconstituents, and mechanisms of action. For this purpose, a literature survey was carried out by using SciFinder, ScienceDirect, Scopus, PubMed, and Web of Science followed by a revision of the bibliographies of the related articles. We have described and analyzed the role of plants in drug discovery and the importance of Nepeta species. Information on the utilization purposes of Nepeta species in folk medicine has been emphasized, and scientific studies on the biological effects and secondary metabolites are addressed. Nepeta species are characterized by terpenoid-type compounds and phenolic constituents, which exert several activities such as an antimicrobial, repellent against major pathogen vector mosquitoes, insecticide, larvicide against Anopheles stephensi, cytotoxic anticarcinogen, antioxidant, anticonvulsant, analgesic, anti-inflammatory agent, and antidepressant, revealing its importance in medicinal and agricultural fields. On the basis of numerous studies, the Nepeta genus demonstrates remarkable therapeutic effects against various diseases. However, clinical studies are warranted to confirm preclinical findings.

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“…( Hordeum vulgare L.) 释放的大麦芽碱( hordenine) 和芦竹碱 ( gramine) 破坏了白芥 ( Sinapis alba) 胚根的细胞壁 [25] [26] ;紫茎泽兰( Eupatorium adenophorum Spreng.) 主效化感物质 DTD 和 HHO 导致受体根尖组织混乱,细胞萎缩,细胞质稀薄,高尔基体和线粒体数量减少 [27] ;Sicyos deppei 水浸提液作用下,受体植物根尖静止中心周围的细胞呈压缩状,根冠细胞中出现无定形和无活性的细胞核、线粒体和内质网,液泡内陷 [24] ;大麦根释放的生物碱使白芥胚根细胞液泡数量增加、体积增大,细胞出现自噬作用,细胞器瓦解 [25] ;化感物质 N鄄phenyl鄄 2鄄 naphthylamine 破坏了小球藻亚细胞结构,导致核固缩,叶绿体结构瓦解 [28] 。质膜和膜性细胞结构是化感物质的作用位点。化感胁迫下,受体植物膜电位发生超极化和去极化,膜透性增加,膜 H + 鄄ATPase 活性受到抑制 [29] 。这类变化与化感胁迫触发活性氧( ROS) 爆发有关。 ROS 是需氧生物正常细胞代谢产生的有害的副产品 [30] 。高浓度的 ROS 具有很强的氧化能力,几乎能与所有的细胞成分发生反应,破坏蛋白质的结构,使 DNA 核苷酸链断裂、嘌呤氧化;破坏蛋白质鄄DNA 交叉连接,并引起膜脂过氧化,导致膜结构的破坏 [20] 。正常情况下,细胞内的 ROS 含量被抗氧化系统控制在最低水平 [30鄄 31] 。但逆境胁迫会导致 ROS 增加,并同时损害抗氧化系统,造成 ROS 积累 [30] 。研究表明,化感胁迫往往伴随氧化胁迫的发生。随着化感物质剂量和处理时间的增加,受体细胞的抗氧化酶活性先升高后降低 [30鄄 36] ,抗氧化酶谱发生变化 [37] ,膜脂过氧化产物丙二醛( MDA) 含量增加 [35鄄 36] ,叶绿体的光合作用和线粒体的呼吸作用降低,导致更多的电子渗漏去还原分子氧( O 2 ) 形成 ROS,加之抗氧化酶活性受抑,引起细胞内大量 ROS 积累,膜巯基减少和膜脂过氧化,破坏了膜的完整性 [38] ,膜性细胞器如叶绿体、线粒体等结构瓦解。 3摇化感胁迫诱导的遗传毒性遗传毒性( Genetic toxicity 或 Genotoxicity) 是指由遗传毒物引起生物细胞基因组分子结构特异改变或使遗传信息发生变化的有害效应,包括 DNA 损伤、基因突变、染色体结构和染色体数目的改变等 [39] 。化感物质可以抑制胸苷掺入或嵌入 DNA [6] ,抑制纺锤体装配,降低有丝分裂指数 [9,27,40鄄 42] ,诱导产生微核及其各种类型 42] , 具有强烈的遗传毒性。唇形科植物 Salvia leucophylla 释放的挥发性单萜抑制了芸苔 ( Brassica campestris) 幼苗根尖顶端组织细胞增殖和 DNA 合成 [43] ;长柔毛野豌豆释放的 Cyanamide( CA) 引起番茄细胞分裂失衡 [22] ;Kekec 等 [44] 运用基于 PCR 扩增的 RAPD 技术根据条带亮度、条带缺失和新条带出现等现象, 证明荆芥 ( Nepeta meyeri ) 精油具有显著的遗传毒性; 一些入侵植物如土荆芥 ( Chenopodium ambrosioides) [9] 、三叶鬼针草 [27] 、银胶菊( Parthenium hysterophorus L.) [38] 、辣子草( Galinsoga parviflora) [40] 、白车轴草( Trifolium repens) [41] 、胜红蓟( Ageratum conyzoides) [42] 等释放的化感物质具有显著的遗传毒性和致畸效应,导致蚕豆根尖细胞有丝分裂指数下降,出现了各类染色体畸变,如染色体断片、染色体桥、染色体环、染色体粘连、染色体滞后、微核、核芽以及三极分裂等。其中,土荆芥挥发油将根尖分生区细胞阻止于有丝分裂 http: / / www.ecologica.cn 前期,表明挥发油抑制了纺锤体微管蛋白的装配 [9] 。 4摇化感胁迫诱导的细胞凋亡细胞凋亡是指由自身特定基因控制的一种细胞死亡类型。由于这种死亡过程受控于遗传基因表达的程序性调控,也称为程序性细胞死亡( PCD) [20] 。在各种逆境中,植物部分细胞发生凋亡以求得整体生存。因此,植物细胞凋亡在抵御逆境胁迫...…”

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Advance in Allelochemical Stress Induced Damage to Plant Cells

Danwei¹,

Yanan²,

Yu³

et al. 2015

Acta Eco Sin

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Allelopathy is a common phenomenon in nature and can be defined as any direct or indirect effects of one plant on the other one (including microbes) through the release of plant produced secondary products (allelochemicals) into the environment by ways of leaching, volatilization, root exudation, and decomposition of residues. When the effect of the allelochemicals is to inhibit the growth and development of receptors, it is considered as a biotic stress called allelochemical stress. The allelochemical can result in the obvious cytotoxicity to receptor plants, affect the generation and survival of root border cells, a population of viable cells that are attached to the root apex in a one鄄cell layer in a manner similar to that of white blood cells functioning in the defense. Allelochemical鄄treated cells exhibit irregular arrangement and shape, and altered the structure of cell walls, plasma membrane, and certain organelles including mitochondria, chloroplasts, Golgi apparatus, vacuoles, etc. The allelochemical also has genotoxicity to receptor plants by interfering with DNA replication, cell division, and gene expression pattern. Chromosomal abnormalities, such as the break, lag, and conglutination of chromosomes, formation of micronucleus and nuclear buds and chromosomal bridges and rings, and three pole divisions was often observed. As a resultant, the mitotic index was significantly reduced in cells exposed to allelochemicals. The allelochemical causes the changes of DNA molecular marker genotype (DNA chain type) in terms of variation in band intensity, or the loss of bands and appearance of new bands. The gene expression pattern in allelochemical鄄treated plants is changed and different genes are up鄄regulated, down鄄regulated, transient鄄expressed, or continuous鄄expressed, respectively. Furthermore, allelochemical treatment enhances the production of reactive oxygen species (ROS) , the toxic metabolic products in plants and other aerobic organisms. However, the activities of ROS scavenging enzymes such as catalase

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Genotoxic effects of catmint (Nepeta meyeriBenth.) essential oils on some weed and crop plants

Cited by 28 publications

References 32 publications

Exogenous salicylic acid alleviates cold damage by regulating antioxidative system in two barley (Hordeum vulgareL.) cultivars

Exogenous salicylic acid alleviates cold damage by regulating antioxidative system in two barley (Hordeum vulgareL.) cultivars

Pharmacological and chemical features of Nepeta L. genus: Its importance as a therapeutic agent

Advance in Allelochemical Stress Induced Damage to Plant Cells

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