Genotoxic effects of metal pollution in two fish species, Oreochromis niloticus and Mugil cephalus, from highly degraded aquatic habitats

Omar, Wael A.; Zaghloul, K.; Abdel-Khalek, Amr Adel; Abo-Hegab, S.

doi:10.1016/j.mrgentox.2012.01.013

Cited by 90 publications

(49 citation statements)

References 35 publications

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“…Hematological and biochemical parameters in fish can significantly change in response to a variety of stressors, and although the changes found in this study suggest water quality as one of the important factors affecting overall physiology of investigated fish, their alterations are nonspecific to a wide range of substances, and thus should always be evaluated in concordance with other indicators and biomarkers. To that effect, micronucleus and erythrocyte nuclear abnormalities assays are frequently used for evaluation of the water pollution (Klobučar et al, 2003;Talapatra and Banerjee, 2007;Pavlica et al, 2008;Omar et al, 2012). The formation of micronuclei is the consequence of chromosomal damage or dysfunction of mitotic apparatus and represents acentral chromosomal fragments or whole chromosomes that have been excluded from the main nucleus during cell division (Udroiu, 2006).…”

Section: Fish Plasma Metabolites and Enzymes Oxidative Stress Genotmentioning

confidence: 99%

Impact of treated wastewater on organismic biosensors at various levels of biological organization

Popović

Strunjak‐Perović

Klobučar

et al. 2015

Science of The Total Environment

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Relating the treated wastewater quality and its impact on organismic biosensors (Prussian carp, Carassius gibelio and earthworm, Eisenia fetida) was the main objective of the study. The impact on health status of fish living downstream, microbiological contamination and antimicrobial resistance, fish tissue structure, blood biochemistry, oxidative stress, genotoxic effects, as well as multixenobiotic resistance mechanism (MXR) was assessed. Treated wastewater discharged from the WWTP modified the environmental parameters and xenobiotic concentrations of the receiving surface waters. Potential bacterial pathogens from fish and respective waters were found in relatively low numbers, although they comprised aeromonads with a zoonotic potential. High resistance profiles were determined towards the tested antimicrobial compounds, mostly sulfamethoxazole and erytrhomycin. Histopathology primarily revealed gill lamellar fusion and reduction of interlamellar spaces of effluent fish. A significant increase in plasma values of urea, total proteins, albumins and triglycerides and a significant decrease in the activity of plasma superoxide dismutase were noted in carp from the effluent-receiving canal. Micronucleus test did not reveal significant differences between the examined groups, but a higher frequency of erythrocyte nuclear abnormalities was found in fish sampled from the effluent-receiving canal. Earthworms indicated to the presence of MXR inhibitors in water and sludge samples, thus proving as a sensitive sentinel organism for environmental pollutants. The integrative approach of this study could serve as a guiding principle in conducting evaluations of the aquatic habitat health in complex bio-monitoring studies.Response to Reviewers: The response is attached as a separate file. We request that this manuscript be considered for publication in Science of the Total Environment as an original, full paper. This manuscript has not been published and is not under consideration for publication in any other journal. All authors concur with the submission.The manuscript is a result of a comprehensive study which integrated various disciplines and research fields. The authors decided to present it as a whole rather than splitting it in two or more papers, for a better understanding of the discussed subject. We believe we have presented a potential guiding principle for conducting evaluations of the aquatic habitat health in complex bio-monitoring studies.I will be serving as the corresponding author for the manuscript. We hope that it is found to be interesting and appropriate for publication in Science of the Total Environment. Should you have any questions, please do not hesitate to contact me.Yours truly, Natalija Topic Popovic, DVM, PhD Response: Thank you for this clarification. We have taken a whole different approach and completely rewritten all sections relating to the PCA as they were indeed confusing, and created a new biplot for plasma parameters. We hope that the new text, as inserted in the respect...

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Section: Fish Plasma Metabolites and Enzymes Oxidative Stress Genotmentioning

confidence: 99%

Impact of treated wastewater on organismic biosensors at various levels of biological organization

Popović

Strunjak‐Perović

Klobučar

et al. 2015

Science of The Total Environment

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“…2). The potential genotoxic role of metals probably contributed to the increase in genotoxic damage, either through interaction of reactive oxygen intermediates and lipid peroxidation products with DNA or to direct interaction of metal with cellular macromolecules forming adducts, alkaline labile sites and strand breaks (Omar et al 2012). Micronucleus frequencies vary according to the degree of environmental stress and could be related to the kind and degree of pollution.…”

Section: Resultsmentioning

confidence: 99%

“…Micronucleus frequencies vary according to the degree of environmental stress and could be related to the kind and degree of pollution. Moreover, other nuclear abnormalities have been used by various authors as good indicators of genotoxicity in fish (Omar et al 2012;Harabawy and Mosleh 2014). Therefore, seven additional nuclear abnormalities were accurately recorded to be potent indicators of genotoxic damage and complement the scoring of MN during the monitoring of genotoxic status.…”

Section: Resultsmentioning

confidence: 99%

“…Lipid peroxidation (LPO) leads to malondialdehyde (MDA) formation, which is the major contributor to the loss of cell function and DNA damage so it was used to express severe oxidative damage (Ruas et al 2008). Moreover, metals can bind to DNA causing the formation of DNA adducts, single and double strand breakages as well as modifications in DNA repair pattern so, genotoxicity of metals have to be evaluated (Omar et al 2012). Micronuclei (MN) counts have been widely employed to biomonitor genotoxcity in wild areas with different levels of contamination.…”

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confidence: 99%

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Antioxidant Responses and Nuclear Deformations in Freshwater Fish, Oreochromis niloticus, Facing Degraded Environmental Conditions

Abdel-Khalek

2015

Bull Environ Contam Toxicol

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Two sites of collection along river Nile, nearby metal-related factories (site2) and 7 km downstream (site3) were compared to unpolluted reference fish farm (site1). Metals concentration (Cu, Zn, Pb, Fe, Mn and Cd) in water and sediment samples showed highly significant (p < 0.01) differences among sites. According to contamination factor and pollution load index values, overall pollution was ordered as site2 > site3 > site1. Compared with Oreochromis niloticus of site1, activities of superoxide dismutase, catalase and glutathione-S-transferase as well as malondialdehyde formation were significantly (p < 0.01) increased in both liver and gills of fish collected from metal contaminated sites. This increment showed a tissue-specific pattern with higher rate of increment in liver than in gills. While reduced glutathione level was sharply decreased in site2 and site3. Micronucleus test was assessed as an environmental genotoxic endpoint in erythrocytes. Assessment of eight nuclear deformations showed gradient frequencies related to the distance from the industrial discharges.

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“…Its low costs and simplicity have led it to be widely applied as a tool or value for studying the general conditions of fish (22). This factor is computed by equation (1).…”

Section: Condition Factor (Cf)mentioning

confidence: 99%

Risk Assessment of Non-Carcinogenic Effects of Heavy Metals from Dez River Fish

Mortazavi

Fard

2017

Iran J Health Sci

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Background and purpose: Considering the many benefits of fish in the food basket, the purpose of this study was to examine the risk assessment effects of heavy metals from Dez river fish. Materials and Methods: 80 samples were caught from the local species of Dez River, they were acid digested; afterwards, they were analyzed by atomic absorption spectrometry. Results:The results showed that there was a positive correlation between accumulating metals and condition factor, especially with regard to zinc and cadmium; this correlation was more likely to be observed for the Capoeta trutta species. Also, comparing the daily and weekly intake of heavy metals with the rate limits of PTDI has shown that the absorption value of metals were considerably lower than the specified limit. Conclusion:The high level of CRlim showed safe consumption of fish in Dez River considering the potential health risk. Zinc and cadmium played an important role in finding out the TTHQ index. The Capoeta trutta and Carasobarbus luteus species had the most and least amounts of TTHQ index. Finally, comparing the means of metal concentrations with standard ones has shown that the concentrations of zinc and cadmium were higher than the global standards.

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Genotoxic effects of metal pollution in two fish species, Oreochromis niloticus and Mugil cephalus, from highly degraded aquatic habitats

Cited by 90 publications

References 35 publications

Impact of treated wastewater on organismic biosensors at various levels of biological organization

Impact of treated wastewater on organismic biosensors at various levels of biological organization

Antioxidant Responses and Nuclear Deformations in Freshwater Fish, Oreochromis niloticus, Facing Degraded Environmental Conditions

Risk Assessment of Non-Carcinogenic Effects of Heavy Metals from Dez River Fish

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