Genotoxic effects of Mycobacterium leprae infection in humans

D’Souza, Doris H.; Das, B. C.

doi:10.1016/0027-5107(94)90241-0

Cited by 8 publications

(2 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several re- [Ahuja, 1991]. Additionally, the damage may be associated with free radicals released by the lymphoid cells ports have demonstrated that bacterial infection can produce genetic damage [D'Souza and Das, 1994]. It is during their attempt to control the infection.…”

Section: Lymphocyte Culturesmentioning

confidence: 99%

Analysis of Mitomycin C-induced micronuclei in lymphocytes from malnourished infected children

Ortiz¹,

Cortés²,

González³

et al. 1997

Environ. Mol. Mutagen.

View full text Add to dashboard Cite

The purpose of this study was to determine if peripheral blood lymphocytes from malnourished children with gastrointestinal or respiratory bacterial infection show increased frequencies of Mitomycin C (MMC)‐induced micronuclei as compared to well‐nourished, infected children. The results indicate that cells from malnourished, infected children had greater chromosome damage. This may indicate that such children would be more susceptible to environmental damage and malignant transformation. Micronucleus frequencies were analyzed in binucleate cells produced by the cytokinesis block method; the overall micronucleus frequency was significantly higher in binucleate cells from malnourished, infected children. The mean micronucleus frequency in MMC‐free cultures was 4.3% in malnourished infected children and 1.0% in well‐nourished infected children. In MMC‐exposed cultures the mean induced micronucleus frequency was 32.6 ± 6.1 vs. 12.9 ± 2.3; 68.6 ± 12.1 vs. 21.0 ± 5.1, and 88.1 ± 16.2 vs. 41.7 ± 5.0 for malnourished and well‐nourished children at 20, 40, and 60 ng/ml MMC, respectively. The number of binucleated cells with more than one micronucleus was also higher in malnourished, infected children at all doses tested, including cells with two micronuclei in MMC‐free cultures from malnourished, infected children. This increase was not found in peripheral blood lymphocytes from well‐nourished infected children. Environ. Mol. Mutagen. 30:363–370, 1997 © 1997 Wiley‐Liss, Inc.

show abstract

Section: Lymphocyte Culturesmentioning

confidence: 99%

Analysis of Mitomycin C-induced micronuclei in lymphocytes from malnourished infected children

Ortiz¹,

Cortés²,

González³

et al. 1997

Environ. Mol. Mutagen.

View full text Add to dashboard Cite

show abstract

“…The basal damage level in both WN and MN children clearly increased with infection. Previous reports demonstrated that certain types of bacterial infections are associated with DNA damage [Rao et al, 1990;D'Souza and Das, 1994], and that these infections are considered as a risk factor for the development of several types of cancer in humans [Canella et al, 1996]. Certain bacterial enzymes can act as DNAases or endonucleases, inducing DNA damage in the cells of infected organisms [Ahuja, 1991]; also free radicals liberated by the lymphoid cells of the infected host, as part of the normal immune response, may increase DNA damage [Halliwell, 1997].…”

Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide–induced DNA damage and DNA repair in lymphocytes from malnourished children

González

Nájera

Cortés

et al. 2002

Environ and Mol Mutagen

View full text Add to dashboard Cite

The aim of this study was to assess DNA repair capacity in lymphocytes of children with protein calorie malnutrition using the single-cell gel electrophoresis (comet) assay. Repair capacity was assessed by estimating the relative decrease of DNA migration length 5, 15, 30, and 60 min after hydrogen peroxide treatment, in three groups of children: well-nourished (WN), well-nourished infected (WN-I), and malnourished infected (MN-I). In addition, the DNA migration length was evaluated in all groups before and after peroxide treatment. Comparison of mean migration lengths observed in WN and WN-I children showed significant differences at all times tested; between WN-I and MN-I differences were also observed, except after hydrogen peroxide exposure. This implies that lymphocytes of WN-I and MN-I children were equally sensitive to hydrogen peroxide. Nevertheless, the MN-I group clearly shows the greatest overall percentage of damaged cells at all times tested. In relation to repair capacity, at 5 min it was approximately 30% in both groups of well-nourished children, but only 20% in MN-I; 15 min after exposure, repair capacity increased to 51% in well-nourished children but only to 31% in MN-I; and at 60 min this capacity increased to 82% in well-nourished but only to 55% in MN-I. These data indicate that lymphocytes of malnourished children show a decreased capacity to repair hydrogen peroxide-induced DNA damage compared to that of well-nourished controls. This reflects that only malnutrition is associated with decreased DNA repair capacity. Additionally, the data confirm that severe infection and malnutrition are two factors clearly associated with increased DNA damage.

show abstract

A rogue cells an indicator of cancer risk due to the action of bacterial restriction endonucleases?

Ahuja

Obe²

1994

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

View full text Add to dashboard Cite

Genotoxic effects of Mycobacterium leprae infection in humans

Cited by 8 publications

References 60 publications

Analysis of Mitomycin C-induced micronuclei in lymphocytes from malnourished infected children

Analysis of Mitomycin C-induced micronuclei in lymphocytes from malnourished infected children

Hydrogen peroxide–induced DNA damage and DNA repair in lymphocytes from malnourished children

A rogue cells an indicator of cancer risk due to the action of bacterial restriction endonucleases?

Contact Info

Product

Resources

About