Genotoxicity and Interference with Cell Cycle Activities by an Ethanolic Extract from Thai Plumbago indica Roots in Human Lymphocytes in vitro

Thitiorul, S; Ratanavalachai, Treetip; Tanuchit, Sermkiat; Itharat, Arunporn; Sakpakdeejaroen, Intouch

doi:10.7314/apjcp.2013.14.4.2487

Cited by 8 publications

(8 citation statements)

References 12 publications

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“…Stock solutions of the ethanolic extracts were prepared at the concentration of 100 mg/mL. Plant materials were standardized using reversed phase HPLC with UV detection to quantify specific biomarker compounds (Bunyong et al ., ; Itharat et al ., ; Plengsuriyakarn et al ., ; Sattaponpan and Kondo, ; Thitiorul et al ., ). The extraction yields (%) including relative amounts of biomarker compounds used are summarized in Table .…”

Section: Methodsmentioning

confidence: 97%

Inhibitory Activities of Thai Medicinal Plants with Promising Activities Against Malaria and Cholangiocarcinoma on Human Cytochrome P450

2015

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Malaria and cholangiocarcinoma remain important public health problems in tropical countries including Southeast Asian nations. Newly developed chemotherapeutic and plant-derived drugs are urgently required for the control of both diseases. The aim of the present study was to investigate the propensity to inhibit cytochrome P450-mediated hepatic metabolism (CYP1A2, CYP2C19, CYP2D6 and CYP3A4) of the crude ethanolic extract of eight Thai medicinal plants with promising activities against malaria and cholangiocarcinoma, using human liver microsomes in vitro. Piper chaba Linn. (PC) and Atractylodes lancea (thung.) DC. (AL) exhibited the most potent inhibitory activities on CYP1A2-mediated phenacetin O-deethylation with mean IC50 of 0.04 and 0.36 µg/mL, respectively. Plumbago indica Linn. (PI) and Dioscorea membranacea Pierre. (DM) potently inhibited CYP2C19-mediated omeprazole 5-hydroxylation (mean IC50 4.71 and 6.92 µg/mL, respectively). DM, Dracaena loureiri Gagnep. (DL) and PI showed the highest inhibitory activities on dextromethorphan O-demethylation (mean IC50 2.93-9.57 µg/mL). PC, DM, DL and PI exhibited the most potent inhibitory activities on CYP3A4-mediated nifedipine oxidation (mean IC50 1.54-6.43 µg/mL). Clinical relevance of the inhibitory potential of DM, PC and PI is of concern for the further development of these plants for the treatment of malaria and/or cholangiocarcinoma.

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Section: Methodsmentioning

confidence: 97%

Inhibitory Activities of Thai Medicinal Plants with Promising Activities Against Malaria and Cholangiocarcinoma on Human Cytochrome P450

2015

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“…Plumbago indica root ethanolic extract of (EEPIR) serves potential therapeutic effects at concentration 25-100µg/mL induced cell kills and cytotoxic effect at concentrations of ≥500µg/mL in-vitro. 33…”

Section: Genotoxic Effectmentioning

confidence: 99%

Phytochemistry and Pharmacological Activities of Plumbago indica L.: An Overview

Hemant¹,

Kumar²,

Ahuja³

2022

IJPI

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Plumbago indica L. has a vast area of pharmacological interest against numerous infectious conditions. Plumbago indica L. is universally used in conventional techniques such as in herbalism, Siddha, Perso-Arabic, and Homeopathy and also in unrestricted generic formulations of the farmer's populations. [1][2][3] Common name -Chitraka (in Ayurvedic), Sheetraj, Chita (in Unani), Chitraka, Chitrakamool (Trade Name). MORPHOLOGYPlumbago indica L. is an ayurvedic remedial plant and it is an evergreen perpetual herb or shrub that grows and spread in warm lush climates up to a height of 1.5meters. It remains stem erect or branched from the base. Leaf parts of Plumbago indica L. are alternate, simple, and oval to elliptic, 8-13cm long, plane, glossy with wavy margins, and have a pointed base. Flowers are bisexual, intense red, 3-5cm long, forming very long-winded, lax spine, and axillary slender reaching 60 cm. The calyx is a tiny, tube-like formation along with ribs covered by prescribed glands with red color. Plumbago indica L. precise with inflorescent throughout a complete year without fruit. [4][5] CHEMICAL CONSTITUENTS AND MEDICINAL PROPERTIESPlumbago indica L. herb part root carry Plumbagin in as active constituent along with other isolated compounds from other parts of the herb including plumbagin, dihydroflavinods, (stem), Chitanone (leaves), glucose, and steroids from flowers, fruits, seeds respectively.Plumbagin shows several pharmacological activities against bacterial infections, antitumor, cardioprotective with several therapeutic effects. Plumbagin additionally acts as an irritant. Therefore, produce stimulant action such as the fast release of the secretions in the central nervous system at low doses and may cause death due to collapse of respiration and paralysis at a high level of doses. Historically as per data reported. Showed anti-implantation and abortion studies reported in animals (rat) due to toxic effects of Plumbagin. Because of its toxic effects, it is a lifethreatening exercise. Reported in-vitro studies declare that Plumbago indica L. have an agent which interferes with mutagenicity. [6][7][8] EXPERIMENTAL PHARMACOLOGY Anti-fungal activityMethanol extract showed strong antifungal activity with zone inhibition of (10.0-27.0) mm while the greatest antifungal activity was found against C. albicans, B. lastomyces dermatitides, and Trichophyton spp. and Microsporum spp C. albicans the more sensitive against fungal strain with the methanol extract of Plumbago indica L. 9 Plumbago indica roots extract with ethanol indicate antifungal activity against Aspergillus niger and C. albicans. 10 The active constituents Plumbagin was reported as fungicidal against C. albicans with MIC and MFC values 0.78 and 1.56µg/mL. 11 Anti-Acne ActivityThe MIC of Plumbago indica roots extraction with acetone against P. acnes, S. epidermidis, and M. furfur was found to be 600, 200, and 300µg/mL. 12 The anti-acne activity showed by acetone extract of Plumbago indica (in gel formulation) was investigated against P. acnes, ...

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“…It is also rarely used as hormicidal poison [7]. P. indica root extract is used to prepare adaptogenic drug for cancer patients in traditional medicinal treatments in Thailand [16]. Due to this vast range of medicinal uses of P. indica, these plants are over exploited form their natural environment and subjected to severe threat of disappearance from its natural habitats.…”

Section: Plate 1 P Indica (A) Plants (B) Flowers (C) Tuberous Rootsmentioning

confidence: 99%

“…In highest concentrations of 500 µg/ml very few mitotic cells were observed. The ability to cause very few mitotic cells signifies high genotoxicity and cytotoxicity which serve well in acting as a chemotherapeutic compound [16]. Several studies have been conducted in relation to prostate cancer and plumbagin [54,55,56].…”

Section: Anti-cancer Activitymentioning

confidence: 99%

Propagation, Phytochemistry and Pharmacology of Plumbago indica - A Review

Priyanjani

Senarath

et al. 2021

JPRI

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Aims: Plumbago indica is a medicinal plant widely grown in the tropics and temperate region to use in traditional systems of medicine. Different parts of this plant are used to treat rheumatoid arthritis, dysmenorrhea, cancer, leprosy, syphilis, rheumatism, paralysis, headache, leukoderma, enlarged glands, scabies, ophthalmia, dyspepsia, haemorrhage, piles, flatulence, loss of appetite etc. Various kinds of researches were carried out in different countries on propagation and pharmacology of this medicinal plant. Scientifically proven data were concentrated on this paper to facilitate reliable convenience data source and encourage further studies. Methodology: Scientific data published on full papers, abstracts about morphology, growth, propagation, traditional medicinal uses and pharmacology of P. indica over 70 years (1949-2020) by researchers in different countries were collected and categorized under suitable topics. Results: According to literature P. indica leaves, stems, roots contain phytochemical compound that are responsible for its medicinal properties. Tissue culture of this plant can be uses as effective propagation method to fulfill the increasing demand of raw materials (dried plant parts) for medicinal preparations as well as preserve the plant in their natural habitat. Value: P. indica is used to treat vast range of diseases in traditional medicinal systems in different countries and currently pharmacological experiments are conducting to prove it scientifically. Plumbagin present in P. indica roots was already identified as a potential anti-cancer agent and subjected to more research interest. Therefore this review article helps new researchers to get wide knowledge about the plant and its pharmacology.

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Genotoxicity and Interference with Cell Cycle Activities by an Ethanolic Extract from Thai Plumbago indica Roots in Human Lymphocytes in vitro

Cited by 8 publications

References 12 publications

Inhibitory Activities of Thai Medicinal Plants with Promising Activities Against Malaria and Cholangiocarcinoma on Human Cytochrome P450

Inhibitory Activities of Thai Medicinal Plants with Promising Activities Against Malaria and Cholangiocarcinoma on Human Cytochrome P450

Phytochemistry and Pharmacological Activities of Plumbago indica L.: An Overview

Propagation, Phytochemistry and Pharmacology of Plumbago indica - A Review

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