Genotypic diversity and virulence markers ofYersinia enterocoliticabiotype 1A strains isolated from clinical and non-clinical origins

Abstract

Yersinia enterocolitica biotype 1A (B1A) strains are considered as non-pathogenic; however, some reports have identified some strains as the causal agents of infection. In South America, few studies molecularly characterized the strains of this biotype. This work typed 51 B1A strains isolated from clinical and non-clinical sources from Brazil and Chile by Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) to elucidate their genotypic diversity, and verify the distribution of 11 virulence markers by PCR. The strains were divided into two groups, ERIC-A and ERIC-B, clustered independently of their clinical or non-clinical origin. No differences were observed in the frequencies of the virulence markers between clinical and non-clinical strains. However, the genes ystB, hreP and myfA occurred exclusively in the strains of the group ERIC-A. Some clinical and non-clinical strains were clustered in the same genetic group and presented the same number of virulence markers, which might suggest the role of the environment and food as a potential source of infection for humans and animals. The results corroborate with the hypothesis that B1A strains are divided into two main clusters that differ in the frequency of some virulence markers, a fact observed for the first time in South American strains.

Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders

“…However, potential loss of gene function, related to horizontal gene transfer cannot be ruled out [20]. The high genotypic diversity of BT1A makes the classification in clinical and non-clinical isolates more problematic since other, yet unknown, virulence factors could be contributing to the observed virulence in some strains [3;22;31]. …”

Molecular and phenotypic characterization of strains of Shigella sonnei isolated over 31 years suggests the circulation of two prevalent subtypes in São Paulo State, Brazil

“…The detection of virulence genes was performed for the 72 strains of S. sonnei (Table 1). The genomic DNA was extracted according to Campioni & Falcão (2014) and the DNA concentrations were determined as described by Sambrook & Russell (2001). The general PCR procedure was performed according to Falcão et al (2006).…”

Draft Genome Sequences of 256 Salmonella enterica subsp. enterica Serovar Enteritidis Strains Isolated from Humans, Food, Chickens, and Farm Environments in Brazil

“…DNA from each strain was extracted according to Campioni et al (8). Libraries were prepared using 1 ng of genomic DNA with the Nextera XT DNA library preparation kit (Illumina, San Diego, CA, USA), and the genomes were sequenced using the NextSeq 500 desktop sequencer with the NextSeq 500/500 high-output version 2 kit (300 cycles) (Illumina, San Diego, CA, USA) for 2 × 151 cycles according to the manufacturer’s instructions.…”