Genotypic prevalence of the fimbrial adhesins (F4, F5, F6, F41 and F18) and toxins (LT, STa, STb and Sbc2e) in<i>Escherichia coli</i>isolated from postweaning pigs with diarrhoea or oedema disease in Korea

Kwon, Donghyok; Choi, Changsun; Jung, Tae-Hwa; Chung, Ho Keun; Kim, J P; Bae, Soyeon; Cho, W S; Kim, J; Chae, Chanhee

doi:10.1136/vr.150.2.35

Cited by 32 publications

(35 citation statements)

References 29 publications

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“…In this work, we found (44%) of F4-positive isolates, in agreement with the high frequency (64.6%) for F4 presented by PCR in the isolates from US (27). A significant proportion (23.9%) of E. coli isolated from 2 to 4-week-old piglets with diarrhoea in the Slovak Republic carried the gene for F4 fimbriae (22 (14); (9.8%) of F4-positive E. coli strains was found in China by agglutination tests (4); 3.75% by PCR (5); and 4.3% in Korea (13).…”

Section: Discussionmentioning

confidence: 99%

Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Vidotto¹,

Lima²,

Fritzen³

et al. 2009

Braz. J. Microbiol.

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Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100 Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, LT and STx2e) by polymerase chain reaction (PCR). The results showed that 60% of the isolates were positive for one or more of the fimbrial antigens and 92% were positive at least for one of the virulence factors examined. Virulence factor genes detected were F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa (40%), STb (47%) and STx2e (3%). Twenty four patterns of virulence factor according to the different virulence genes form were found and the most frequent virulence gene pattern was F4, F18, F41, STa, STb and LT. Most of the isolates that carried genes for adhesins also harboured genes for toxins.

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Section: Discussionmentioning

confidence: 99%

Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Vidotto¹,

Lima²,

Fritzen³

et al. 2009

Braz. J. Microbiol.

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“…Therefore, determining antibiotic patterns would be useful in understanding and controlling ETEC infection in swine. 7,11 There are some reports in which virulence profiling and antibiogram of E. coli isolates from pigs were studied, 4,11,12,14,15,25 but only in a few cases have comparative results of both been shown. In the current study, genetic relationships between E. coli strains isolated from piggeries were determined by examining both virulence profiling and antibiotic resistance patterns.…”

Section: Adherence Factors (F1 [Type 1 Fimbriae] F4 [K88] F5mentioning

confidence: 99%

Genotypes, Antibiogram, and Pulsed-Field Gel Electrophoresis Profiles of Escherichia Coli Strains from Piglets in Korea

et al. 2009

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Abstract. Adherence factors and enterotoxins are major virulence factors of enterotoxigenic Escherichia coli (ETEC). Antibiotics have been used frequently for the treatment and prevention of ETEC infection in piggeries worldwide, including Korea. Therefore, data on both virulence profiles and antibiotic resistance patterns are useful in the epidemiological study of ETEC. A total number of 198 E. coli field isolates were examined. The most prevalent pathotype was F1, followed by a combination of F1 and EAST1. All of the 71 isolates were resistant to more than 2 antibiotics used in a disk diffusion test, and 87.94% of the isolates were found to be resistant to more than 4 antibiotics. Investigations were also conducted to correlate the virulence gene profiles with antibiogram and pulsed-field gel electrophoresis (PFGE). Although a high degree of polymorphism was noted among strains having the same virulence patterns, the highest similarity pattern was observed carrying the same virulence profiles and similar antibiogram. Thus, investigation of both virulence profiles and antibiogram is essential to the epidemiological study of ETEC. Moreover, the PFGE method might be applicable as a tool to reveal genetic relatedness among E. coli strains from piggeries in Korea.

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“…F4 causes more severe diarrhoea in weaned piglets, causing postweaning diarrhoea, but is also associated with diarrhoea in nursing pigs. The F5, F6 and F41 genes are usually associated with ETEC from neonatal pigs (between 4 and 14 days of age), but may be associated less frequently with diarrhoea in older pre-weaned pigs (Kwon et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

Toledo

Gómez

Cruz

et al. 2012

Journal of Medical Microbiology

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Faecal Escherichia coli isolates from suckling (n5503) and weaning (n5450) piglets with and without diarrhoea from 10 farms in Mexico were examined for identification and prevalence of virulence genes. E. coli isolates were tested further for enterotoxin (LT, STa, STb, Stx1, Stx2 and EAST1), fimbrial (F4, F5, F6, F17, F18 and F41) and eae adhesin genes by multiplex PCR. Of the 953 isolates of E. coli examined by multiplex PCR, 650 (68.2 %) isolates were positive for at least one adhesin gene. Among the isolates from diarrhoeic piglets, F41 (72 %) was the most prevalent adhesin followed by eae adhesin (27 %), F6 (12 %), F17 (9 %), F18 (9 %), F5 (8 %) and F4 (3 %). Enterotoxin genes were detected in 424 (44.5 %) of the isolates, of which EAST1 (38 %) and STa (30 %) were the most common, followed by STb (17 %), Stx2 (6 %) and LT (5 %). Twenty-three per cent of isolates from suckling piglets and 43 % of isolates from weaned piglets carried both enterotoxin and adhesin genes, the most common virotypes being F41 : STa, F41 : EAST1, EAST1 : eae, F41 : F6, F41 : STa : STb, F41 : eae and F17 : eae. The present study examined for the first time, to our knowledge, the prevalence of 13 virulence genes among E. coli strains isolated from piglets with and without diarrhoea in Mexico. The results suggested that there are a wide variety of virulence genes associated with diarrhoea in piglets. This study provides baseline information on the significance of specific virotypes associated with suckling and weaning periods in piglets in Mexico.

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Genotypic prevalence of the fimbrial adhesins (F4, F5, F6, F41 and F18) and toxins (LT, STa, STb and Sbc2e) inEscherichia coliisolated from postweaning pigs with diarrhoea or oedema disease in Korea

Cited by 32 publications

References 29 publications

Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Genotypes, Antibiogram, and Pulsed-Field Gel Electrophoresis Profiles of Escherichia Coli Strains from Piglets in Korea

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

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