Genotyping of Human Platelet Antigen-1 to -5 and -15 by Polymerase Chain Reaction with Sequence-specific Primers (PCR-SSP) and Real-time PCR in Azeri Blood Donors

Azizi, Seyed Ghader; Samiee, Shahram; Shaiegan, Mojgan; Zadsar, Maryam

doi:10.18502/ijaai.v20i3.6333

Cited by 3 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering that the frequency of HPAs polymorphisms regardless of the ethnicity has been studied in Iran so far,[ 3 5 7 10 ] the existence of such differences in the frequency of platelet antigen systems in the present study (Turkmen ethnicity) with other studies in the country can be attributed to racial differences that need further investigation in this regard.…”

Section: Discussionmentioning

confidence: 70%

Frequency of Human Platelet Antigens −1 to −5 and −15 in Turkmen Blood Donors

Nozarimirarkolaei

Dadashi

Ghasemi

et al. 2023

Advanced Biomedical Research

View full text Add to dashboard Cite

Background: Due to the presence of platelet antigen polymorphisms, human platelet membrane glycoproteins can be identified as an alloantigen or autoantigen. The aim of this study was to determine the frequencies of human platelet antigens (HPAs)-1 to-5 and-15 in Turkmen blood donors and establish a panel of accredited HPAs negative donors as well as an HPA-typed platelet donor registry. Materials and Methods: HPA-1 to-5 and-15 typing was performed by the polymerase chain reaction-sequence-specific primer techniques on 80 unrelated Turkmen donors who were referred to Aq-Qala Blood Transfusion Center in Golestan Province from September 2018 to October 2019. Results: The frequencies of HPA phenotypes were determined as follows: HPA-1aa: 92.5%, HPA-1ab: 7.5%, HPA-2aa: 77.5%, HPA-2ab: 20.0%, HPA-2bb: 2.5%, HPA-3aa: 75.3%, HPA-3ab: 50%, HPA-3bb: 11.2%, HPA-4aa: 100%, HPA-5aa: 78.5%, HPA-5ab: 21.5%, HPA-15aa: 41.2%, HPA-15ab: 56.2% and HPA-15bb: 17.5%. Conclusion: Determining the genotype of HPAs that play an important role in platelet refractory can improve the management of alloimmunization due to the incompatibility of HPAs between the recipients and donors. Therefore, the registration process for national platelet donors can help patients accelerate and improve the quality of transfused platelets.

show abstract

Section: Discussionmentioning

confidence: 70%

Frequency of Human Platelet Antigens −1 to −5 and −15 in Turkmen Blood Donors

Nozarimirarkolaei

Dadashi

Ghasemi

et al. 2023

Advanced Biomedical Research

View full text Add to dashboard Cite

show abstract

“…Therefore, only HLA-A and HLA-B loci were routinely analyzed to search for HLA-matched/compatible platelet units ( 20 ). The HPA genotyping methods included PCR-SSP, TaqMan assay, PCR-SBT, and NGS ( 22 , 23 , 33 , 34 ). However, there is a rare study for HPA and HLA genotyping simultaneously.…”

Section: Discussionmentioning

confidence: 99%

“…In order to genotype for HLA-typed and/or HPA-typed unrelated donors, it needs to provide the corresponding methods for HLA-A and HLA-B loci, and HPA systems. Currently, many methods have been developed to detect HLA-A and HLA-B loci and HPA systems, including PCR sequence-specific primers (PCR-SSP), PCR sequence-based typing (PCR-SBT), and real-time PCR ( 11 , 21 – 23 ). However, these methods always detect single gene separately and need too many wells and multiple amplification for the test.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous genotyping for human platelet antigen systems and HLA-A and HLA-B loci by targeted next-generation sequencing

Wang

You²,

He³

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

In order to treat the alloimmunization platelet transfusion refractoriness (PTR), human leukocyte antigen (HLA)-type and/or human platelet antigen (HPA)-type matched platelets between donors and patients are usually used. Therefore, genotyping of HLA-A and HLA-B loci, as well as HPA systems, for donors and patients, is of great significance. However, there is a rare report of genotyping for HLA-A and HLA-B loci as well as HPA systems at the same time. In this study, a high-throughput method for simultaneous genotyping of HLA-A and HLA-B loci, as well as HPA genotyping, was developed. A RNA capture probe panel was designed covering all exon sequences of the GP1BA, GP1BB, ITGA2, CD109, ITGB3, and ITGA2B genes and HLA-A and HLA-B loci. The HLA-A, HLA-B, and 34 HPA systems were genotyped using a targeted next-generation sequencing (NGS) method. The genotypes of the HLA-A and HLA-B loci, as well as the HPA, were assigned based on the nucleotides in the polymorphism sites. Using the NGS method, 204 unrelated blood specimens were successfully genotyped for all 34 HPA systems as well as HLA-A and HLA-B loci. The accuracy of the NGS method was 100%. Only HPA-2, HPA-3, HPA-5, HPA-6w, HPA-15, and HPA-21w showed polymorphism with frequencies of 0.9412, 0.6863, 0.9853, 0.9779, 0.4314, and 0.9951 for a allele, respectively. Thirty-two single nucleotide variants (SNVs) were detected. Of them, 12 SNVs can lead to amino acid change. HLA-A*11:01 and HLA-B*46:01 are the most common alleles for HLA-A and HLA-B loci. A targeted next-generation sequencing method for simultaneously genotyping HPA systems and HLA-A and HLA-B loci was first established, which could be used to create a database of HLA-typed and/or HPA-typed unrelated donors.

show abstract

“…In recent years, a large diversity of HPA genotypes have been reported from varied human ethnic groups from a variety of geographical locations (Azizi et al., 2021; Hayashi et al., 2020; Jovanovic Srzentic et al., 2021; Kazemi et al., 2020; Silva‐Malta et al., 2018; Yan et al., 2006). Many of these studies have suggested significant differences in the frequencies between populations.…”

Section: Introductionmentioning

confidence: 99%

Genetic landscape of human platelet antigen variants in the Indian population analysed from 1029 whole genomes

Bhoyar

Imran

Senthivel

et al. 2023

Int J Immunogenetics

View full text Add to dashboard Cite

Genetic variants in human platelet antigens (HPAs) considered allo‐ or auto antigens are associated with various disorders, including neonatal alloimmune thrombocytopenia, platelet transfusion refractoriness and post‐transfusion purpura. Although global differences in genotype frequencies were observed, the distributions of HPA variants in the Indian population are largely unknown. This study aims to explore the landscape of HPA variants in India to provide a basis for risk assessment and management of related complications. Population‐specific frequencies of genetic variants associated with the 35 classes of HPAs (HPA‐1 to HPA‐35) were estimated by systematically analysing genomic variations of 1029 healthy Indian individuals as well as from global population genome datasets. Allele frequencies of the most clinically relevant HPA systems in the Indian population were found as follows, HPA‐1a – 0.884, HPA‐1b – 0.117, HPA‐2a – 0.941, HPA‐2b – 0.059, HPA‐3a – 0.653, HPA‐3b – 0.347, HPA‐4a – 0.999, HPA‐4b – 0.0010, HPA‐5a – 0.923, HPA‐5b – 0.077, HPA‐6a – 0.998, HPA‐6b – 0.002, HPA‐15a – 0.582 and HPA‐15b – 0.418. This study provides the first comprehensive analysis of HPA allele and genotype frequencies using large scale representative whole genome sequencing data of the Indian population.

show abstract

Genotyping of Human Platelet Antigen-1 to -5 and -15 by Polymerase Chain Reaction with Sequence-specific Primers (PCR-SSP) and Real-time PCR in Azeri Blood Donors

Cited by 3 publications

References 24 publications

Frequency of Human Platelet Antigens −1 to −5 and −15 in Turkmen Blood Donors

Frequency of Human Platelet Antigens −1 to −5 and −15 in Turkmen Blood Donors

Simultaneous genotyping for human platelet antigen systems and HLA-A and HLA-B loci by targeted next-generation sequencing

Genetic landscape of human platelet antigen variants in the Indian population analysed from 1029 whole genomes

Contact Info

Product

Resources

About