Genotyping of rotavirus isolates collected from children less than 5 years of age admitted for diarrhoea at the Yangon Children's Hospital, Myanmar

Moe, Kyaw Kyaw; Thu, Hlaing Myat; Oo, Win Mar; Aye, Khin Mar; Shwe, Tin; Mar, Win; Kirkwood, Carl D.

doi:10.1016/j.vaccine.2009.08.068

Cited by 21 publications

(14 citation statements)

References 20 publications

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“…These findings support the need for continuous surveillance of the disease in various locations. Our study found a high incidence of rotavirus infection in Yogyakarta, Indonesia, which is comparable to the findings of similar studies in surrounding countries, such as Malaysia (38%) (21), Thailand (43%) (22), Cambodia (56%) (23) and Myanmar (57%) (24). Our study provides evidence that rotavirus infection gives a substantial burden to the one sample was identified as G3.…”

Section: From February To August 2009 104supporting

confidence: 88%

Identification of Rotavirus Strains Causing Diarrhoea in Children under Five Years of Age in Yogyakarta, Indonesian

Nirwati

Hakim

Aminah³

et al. 2017

MJMS

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Background: Rotavirus is an important cause of severe diarrhoea in children. The aims of this study were to identify the rotavirus strains that cause diarrhoea in children in Yogyakarta and to determine the association between rotavirus positivity and its clinical manifestations.Methods: Clinical data and stool samples were collected from children hospitalised at Kodya Yogyakarta Hospital, Indonesia. Rotavirus was detected in stool samples using an enzyme immunoassay (EIA), which was followed by genotyping using reverse transcriptase polymerase chain reaction (RT-PCR). Electropherotyping was performed for the rotavirus-positive samples.Results: In total, 104 cases were included in the study, 57 (54.8%) of which were rotavirus-positive. Based on a multiple logistic regression analysis, age group, vomiting and stool mucous were associated with rotavirus positivity. Most of the 56 samples subjected to genotyping were classified as G1 (80.36%) and P[8] (69.64%) genotypes. The genotype combination G1P[8] was identified as the most prevalent strain (66.07%). Of the 19 samples subjected to electropherotyping, 17 G1 isolates and 1 G3 isolate had long patterns, and 1 G1 isolate had a short pattern.Conclusion: G1P[8] was the most dominant strain of rotavirus causing diarrhoea in children in Yogyakarta. Age group, vomiting and stool mucous were associated with rotavirus positivity.

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Section: From February To August 2009 104supporting

confidence: 88%

Identification of Rotavirus Strains Causing Diarrhoea in Children under Five Years of Age in Yogyakarta, Indonesian

Nirwati

Hakim

Aminah³

et al. 2017

MJMS

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“…Although global surveys on the G and P types of human rotaviruses have been extensively performed, there have been few epidemiological studies on human rotaviruses in Myanmar (4,5). In this study, we characterized human rotaviruses in Myanmar by genotyping the VP7 and VP4 genes, and found a high prevalence of G2 and unusual G12.…”

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confidence: 89%

“…Although the occurrence of G and P types Prevalence of G12 Human Rotavirus in Myanmar tends to differ year by year and district by district, G1P [8] has been shown to be the most common, followed by G2P [4], G3P [8], G4P [8], and G9P [8] worldwide (3). In Myanmar, the G and P prevalence has not been examined except in 1 study performed in 2004 5 (5). In that study, G3P [8] was the most common (27z), followed by G1P [8] (19z) and G2P [4](11z).…”

mentioning

confidence: 99%

High Prevalence of G12 Human Rotaviruses in Children with Gastroenteritis in Myanmar

et al. 2016

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SUMMARY: Human rotavirus samples from 54 children with acute gastroenteritis in Myanmar in 2011 were subjected to reverse transcription-PCR to determine their G and P types. On G typing, G2 (24/54; 44.4z) was found to be the most prevalent, followed by G12 (17/54; 31.5z) and G1 (1/54; 1.9z). Mixed cases with G2 and G12 were found in 12 of the 54 (22.2z) samples. On P typing, P[4] was found to be the most predominant (29/54; 53.7z), followed by P[8] (17/54; 31.5z) and P[6] (4/54; 7.4z). Mixed cases with P[4] and P [8] were detected in 4 of 54 (7.4z) samples. Thus, occurrence of G2 and unusual G12 in high proportions was characteristic of human rotaviruses in Myanmar in this study setting.Human group A rotaviruses are the major pathogens causing acute non-bacterial gastroenteritis in infants and young children worldwide. It has been estimated that rotavirus infections are responsible for the deaths of approximately 400,000 children per year in developing countries (1). Also in developed countries, rotavirus gastroenteritis is associated with severe cases requiring hospitalization. Furthermore, some of central nervous system disorders, including encephalopathy, appear to be related to rotavirus infections. With this background, 2 live rotavirus vaccines have been licensed worldwide and are included in the scheduled immunization programs in many countries.Two rotavirus outer capsid proteins, VP7 and VP4, are independently associated with the G and P types, respectively. A cutoff value of 80z nucleotide sequence identity has been employed for defining distinct G and P types (2). At least 27 G types and 37 P types have been reported to date in mammalian and avian species. In humans, at least 10 G types and 10 P types have been detected. G1 to G4 and G9 are the major G types, with G5, G8, and G12 being the unusual ones, while, P[8] is the most common, followed by P[4], P[6], and P[9]. G1P[8] is the most frequent G and P combination in the rotaviruses isolated from symptomatic humans worldwide (3).Although global surveys on the G and P types of human rotaviruses have been extensively performed, there have been few epidemiological studies on human rotaviruses in Myanmar (4,5). In this study, we characterized human rotaviruses in Myanmar by genotyping the VP7 and VP4 genes, and found a high prevalence of G2 and unusual G12.Stool specimens were collected from children with gastroenteritis in the Defense Services Obstetrics, Gynecology and Children Hospital, Yangon, Myanmar. The ages of children ranged from 2 months to 3 years. Fifty-four rotavirus-positive diarrheal children selected randomly were enrolled in a double-blind, placebocontrolled clinical trial of rotavirus-specific IgY conducted between January 2011 and March 2011 (6). Stool suspensions (10z) were prepared from the stool specimens collected from the 54 children before the start of the trial.For G typing and P typing, rotavirus double-stranded RNAs extracted from the stool suspensions were used as templates for reverse transcription-PCR (RT-PCR) in 2 steps (fi...

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“…Rotavirus infection is understood to disrupt P-bodies, the sites of concentration of the RNA degradation machinery. Rotavirus is an RNA virus which causes acute diarrhoea across many developing countries, mainly affecting young children, with its spread being attributable to poor sanitation and hygiene [54]. This virus is a double-stranded, nonenveloped virus which utilises rotavirus protein NSP1 to degrade the deadenylase, PAN3, in the Pbodies, resulting in transcript stabilisation, an effect also seen in Poliovirus [49].…”

Section: Xrn1 and The Host Response To Virusesmentioning

confidence: 99%

The roles of the exoribonucleases DIS3L2 and XRN1 in human disease

Pashler

Towler

Jones

et al. 2016

Biochemical Society Transactions

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RNA degradation is a vital post-transcriptional process which ensures that transcripts are maintained at the correct level within the cell. DIS3L2 and XRN1 are conserved exoribonucleases which are critical for the degradation of cytoplasmic RNAs. Although the molecular mechanisms of RNA degradation by DIS3L2 and XRN1 have been well studied, less is known about their specific roles in development of multicellular organisms or human disease. This review focusses on the roles of DIS3L2 and XRN1 in the pathogenesis of human disease, particularly in relation to phenotypes seen in model organisms. The known diseases associated with loss of activity of DIS3L2 and XRN1 are discussed, together with possible mechanisms and cellular pathways leading to these disease conditions.

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Genotyping of rotavirus isolates collected from children less than 5 years of age admitted for diarrhoea at the Yangon Children's Hospital, Myanmar

Cited by 21 publications

References 20 publications

Identification of Rotavirus Strains Causing Diarrhoea in Children under Five Years of Age in Yogyakarta, Indonesian

Identification of Rotavirus Strains Causing Diarrhoea in Children under Five Years of Age in Yogyakarta, Indonesian

High Prevalence of G12 Human Rotaviruses in Children with Gastroenteritis in Myanmar

The roles of the exoribonucleases DIS3L2 and XRN1 in human disease

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