Geobacillus stearothermophilus V ubiE gene product is involved in the evolution of dimethyl telluride in Escherichia coli K-12 cultures amended with potassium tellurate but not with potassium tellurite

Araya, Manuel A.; Swearingen, Jerry W.; Plishker, Mary F.; Saavedra, Claudia P.; Chasteen, Thomas G.; Vásquez, Claudio C.

doi:10.1007/s00775-004-0554-z

Cited by 22 publications

(18 citation statements)

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“…CysK catalyzes the last step of inorganic sulfur fixation into L-cysteine, while SUMT is involved in the biosynthesis of siroheme, an essential sulfite reductase cofactor that participates in the inorganic assimilation of sulfur (15). We recently demonstrated that cobA and ubiE genes from G. stearothermophilus V confer increased tolerance to oxyanions of selenium and tellurium when expressed in E. coli (1,32). Finally, IscS, which yields sulfur and L-alanine from L-cysteine, has been shown to be involved, along with IscA and IscU, in the recovery of [Fe-S] clusters (9,29).…”

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Cysteine Metabolism-Related Genes and Bacterial Resistance to Potassium Tellurite

Fuentes

Castro

et al. 2007

J Bacteriol

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Tellurite exerts a deleterious effect on a number of small molecules containing sulfur moieties that have a recognized role in cellular oxidative stress. Because cysteine is involved in the biosynthesis of glutathione and other sulfur-containing compounds, we investigated the expression of Geobacillus stearothermophilus V cysteinerelated genes cobA, cysK, and iscS and Escherichia coli cysteine regulon genes under conditions that included the addition of K 2 TeO 3 to the culture medium. Results showed that cell tolerance to tellurite correlates with the expression level of the cysteine metabolic genes and that these genes are up-regulated when tellurite is present in the growth medium.Sulfur is an essential element that is required for the biosynthesis of proteins, enzyme cofactors, and other important biomolecules. In bacteria, this element can be assimilated into sulfur-containing amino acids through enzymatic fixation from inorganic sources, such as sulfate and/or thiosulfate (15, 38). Although tellurium shares several chemical properties with sulfur, no biological function for Te is known to date. Conversely, some tellurium compounds, like the oxyanion tellurite (TeO 3 2Ϫ ), are extremely toxic for most forms of life, especially microorganisms (34).It has been proposed that K 2 TeO 3 toxicity could be due to the oxidation of cellular thiols such as glutathione (37) or the generation of superoxide radical during tellurite reduction, which would cause a redox imbalance resulting in intracellular oxidative stress (5,23,26,33,34,36).Maintenance of cell redox balance is one of the most important processes involving molecules synthesized from reduced sulfur taken from the environment. Glutathione (GSH) is one of the major nonprotein thiols in living organisms, including humans, yeast, and bacteria (6, 10). GSH has been involved in resistance to osmotic and oxidative stress as well as in Escherichia coli resistance to the toxic effects of electrophiles like methylglyoxal (6,11,31). A protective effect of GSH against oxidative stress has also been described for Lactococcus lactis and Rhodobacter capsulatus (17,18).Three genes involved in tellurite resistance have been described previously for the thermotolerant gram-positive rod Geobacillus stearothermophilus V (27, 33, 41). The genes that are involved in the metabolism of cysteine are cysK, iscS, and cobA, and they encode a cysteine synthase (CysK), a cysteine desulfurase (IscS), and a uroporphyrinogen-III C-methyltransferase (SUMT), respectively. CysK catalyzes the last step of inorganic sulfur fixation into L-cysteine, while SUMT is involved in the biosynthesis of siroheme, an essential sulfite reductase cofactor that participates in the inorganic assimilation of sulfur (15). We recently demonstrated that cobA and ubiE genes from G. stearothermophilus V confer increased tolerance to oxyanions of selenium and tellurium when expressed in E. coli (1, 32). Finally, IscS, which yields sulfur and L-alanine from L-cysteine, has been shown to be involved, along with IscA ...

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Cysteine Metabolism-Related Genes and Bacterial Resistance to Potassium Tellurite

Fuentes

Castro

et al. 2007

J Bacteriol

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“…Multiple test tubes containing the precultures of genetically modified 1VH (Araya et al, 2004) amended with tellurite to yield a final concentration of 0.05 mM were prepared.…”

Section: Part 2: Capillary Electrophoretic Methods Developmentmentioning

confidence: 99%

“…Bioremediation of metalloid-amended cultures in laboratory settings using metalloidresistant microorganisms such as Pseudomonas fluorescens K27 (Zhang and Chasteen, 1994;Hapuarachchi et al, 2004) and more recently genetically-modified E. coli with genes from a metalloid-resistant thermophile (Araya et al, 2004;Swearingen et al, 2006) has been reported. The production path of volatile selenium compounds observed in the headspace of selenite amended cultures agrees well with the following scheme, redrawn from Ganther and Lawrence (1997 Bioaccumulation of selenium oxyanions and selenocyanate has also been reported in one study using selenium-resistant plants in a successful effort at phytoremediation (de Souza et al, 2002).…”

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confidence: 99%

“…Bacterial cultures of Escherichia coli K-12 carrying the recombinant plasmid p1VH were used as described elsewhere (Araya et al, 2004).…”

Section: Isolation and Identification Of Bacteriamentioning

confidence: 99%

“…Bacterial cultures of a genetically-modified clone of E. coli K-12 (1VH) were grown anaerobically at 37 o C in LB (Lauria-Bertani) medium for 24 h as described elsewhere (Araya et al, 2004). LB medium was prepared as follows.…”

Section: Bacterial Cultures and Growth Mediamentioning

confidence: 99%

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Capillary electrophoretic determination of selenocyanate and selenium and tellurium oxyanions in bacterial cultures

Pathem

Pradenas

Castro

et al. 2007

Analytical Biochemistry

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Experiments were also designed to detect the biological production of selenocyanate in Se-amended bacterial cultures using IC-ICP-MS. A selenium-resistant bacterium, 130404, when amended with 1.0 mM selenate biologically produced selenocyanate in replicate cultures grown to stationary phase. The amounts produced were statistically significantly different than the amounts of SeCN -produced in sterile but warmed growth media. The headspace gases above growing cultures with or without metalloid amendments were analyzed using gas chromatography. Organo-selenium iii compounds that were detected were methaneselenol, dimethyl selenide, dimethyl selenenyl sulfide, dimethyl diselenenyl sulfide, dimethyl diselenide. Two late eluting peaks were observed in the same cultures that were not characterized but can reasonably be inferred to be dimethyl diselenenyl sulfide and dimethyl triselenide.

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Pathways and Substrate Specificity of DMSP Catabolism in Marine Bacteria of the Roseobacter Clade

2010

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The volatiles released by Phaeobacter gallaeciensis, Oceanibulbus indolifex and Dinoroseobacter shibae have been investigated by GC-MS, and several MeSH-derived sulfur volatiles have been identified. An important sulfur source in the oceans is the algal metabolite dimethylsulfoniopropionate (DMSP). Labelled [2H6]DMSP was fed to the bacteria to investigate the production of volatiles from this compound through the lysis pathway to [2H6]dimethylsulfide or the demethylation pathway to [2H3]-3-(methylmercapto)propionic acid and lysis to [2H3]MeSH. [2H6]DMSP was efficiently converted to [2H3]MeSH by all three species. Several DMSP derivatives were synthesised and used in feeding experiments. Strong dealkylation activity was observed for the methylated ethyl methyl sulfoniopropionate and dimethylseleniopropionate, as indicated by the formation of EtSH- and MeSeH-derived volatiles, whereas no volatiles were formed from dimethyltelluriopropionate. In contrast, the dealkylation activity for diethylsulfoniopropionate was strongly reduced, resulting in only small amounts of EtSH-derived volatiles accompanied by diethyl sulfide in P. gallaeciensis and O. indolifex, while D. shibae produced the related oxidation product diethyl sulfone. The formation of diethyl sulfide and diethyl sulfone requires the lysis pathway, which is not active for [2H6]DMSP. These observations can be explained by a shifted distribution between the two competing pathways due to a blocked dealkylation of ethylated substrates.

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Geobacillus stearothermophilus V ubiE gene product is involved in the evolution of dimethyl telluride in Escherichia coli K-12 cultures amended with potassium tellurate but not with potassium tellurite

Cited by 22 publications

References 42 publications

Cysteine Metabolism-Related Genes and Bacterial Resistance to Potassium Tellurite

Cysteine Metabolism-Related Genes and Bacterial Resistance to Potassium Tellurite

Capillary electrophoretic determination of selenocyanate and selenium and tellurium oxyanions in bacterial cultures

Pathways and Substrate Specificity of DMSP Catabolism in Marine Bacteria of the Roseobacter Clade

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