Geobacter
            
            luticola sp. nov., an Fe(III)-reducing bacterium isolated from lotus field mud

Viulu, Samson; Nakamura, Kohei; Okada, Yurina; Saitou, Sakiko; Takamizawa, Kazuhiro

doi:10.1099/ijs.0.039321-0

Cited by 25 publications

(20 citation statements)

References 34 publications

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“…This organism was most closely related to Geobacter luticola . Although, G. luticola was shown to reduce nitrate to N 2 O (Viulu et al, 2013) and no DNRA activity was reported, the related organism found in this study is most likely performing the DNRA in our system.…”

Section: Discussioncontrasting

confidence: 54%

DNRA and Denitrification Coexist over a Broad Range of Acetate/N-NO3− Ratios, in a Chemostat Enrichment Culture

et al. 2016

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Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) compete for nitrate in natural and engineered environments. A known important factor in this microbial competition is the ratio of available electron donor and elector acceptor, here expressed as Ac/N ratio (acetate/nitrate-nitrogen). We studied the impact of the Ac/N ratio on the nitrate reduction pathways in chemostat enrichment cultures, grown on acetate mineral medium. Stepwise, conditions were changed from nitrate limitation to nitrate excess in the system by applying a variable Ac/N ratio in the feed. We observed a clear correlation between Ac/N ratio and DNRA activity and the DNRA population in our reactor. The DNRA bacteria dominated under nitrate limiting conditions in the reactor and were outcompeted by denitrifiers under limitation of acetate. Interestingly, in a broad range of Ac/N ratios a dual limitation of acetate and nitrate occurred with co-occurrence of DNRA bacteria and denitrifiers. To explain these observations, the system was described using a kinetic model. The model illustrates that the Ac/N effect and concomitant broad dual limitation range related to the difference in stoichiometry between both processes, as well as the differences in electron donor and acceptor affinities. Population analysis showed that the presumed DRNA-performing bacteria were the same under nitrate limitation and under dual limiting conditions, whereas the presumed denitrifying population changed under single and dual limitation conditions.

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Section: Discussioncontrasting

confidence: 54%

DNRA and Denitrification Coexist over a Broad Range of Acetate/N-NO3− Ratios, in a Chemostat Enrichment Culture

et al. 2016

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“…Members of the genus Geobacter are often the predominant Fe(III)-reducing bacteria in subsurface anoxic environments (Lovley et al, 2004) and many species of the genus Geobacter have been isolated from hydrocarbon-contaminated environments and freshwater aquatic environments (Coates et al, 1996;Kunapuli et al, 2010;Prakash et al, 2010;Shelobolina et al, 2008;Viulu et al, 2013b).…”

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confidence: 99%

“…Total Fe(II) was extracted with 0.5 mM HCl for 1.5 h and quantified colorimetrically using 1,10-phenanthroline as described by Wu et al (2010). Similar to known species (Viulu et al, 2013b) of the genus Geobacter, strain GSS01 T conserved energy for growth while coupling the reduction of amorphous ferric oxide ferrihydrite to the oxidation of acetate (Fig. S2).…”

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confidence: 99%

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Geobacter soli sp. nov., a dissimilatory Fe(III)-reducing bacterium isolated from forest soil

Zhou

Yang

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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A novel Fe(III)-reducing bacterium, designated GSS01 T , was isolated from a forest soil sample using a liquid medium containing acetate and ferrihydrite as electron donor and electron acceptor, respectively. Cells of strain GSS01 T were strictly anaerobic, Gram-stain-negative, motile, nonspore-forming and slightly curved rod-shaped. Growth occurred at 16-40 6C and optimally at 30 6C. The DNA G+C content was 60.9 mol%. The major respiratory quinone was MK-8. The major fatty acids were C 16 : 0 , C 18 : 0 and C 16 : 1 v7c/C 16 : 1 v6c. Strain GSS01 T was able to grow with ferrihydrite, Fe(III) citrate, Mn(IV), sulfur, nitrate or anthraquinone-2,6-disulfonate, but not with fumarate, as sole electron acceptor when acetate was the sole electron donor. The isolate was able to utilize acetate, ethanol, glucose, lactate, butyrate, pyruvate, benzoate, benzaldehyde, mcresol and phenol but not toluene, p-cresol, propionate, malate or succinate as sole electron donor when ferrihydrite was the sole electron acceptor. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain GSS01 T was most closely related to Geobacter sulfurreducens PCA T (98.3 % sequence similarity) and exhibited low similarities (94.9-91.8 %) to the type strains of other species of the genus Geobacter. The DNA-DNA relatedness between strain GSS01 T and G. sulfurreducens PCA T was 41.4±1.1 %. On the basis of phylogenetic analysis, phenotypic characterization and physiological tests, strain GSS01 T is believed to represent a novel species of the genus Geobacter, and the name Geobacter soli sp. nov. is proposed. The type strain is GSS01 T (5KCTC 4545 T 5MCCC 1K00269 T ).

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“…Similarly, the abundance of Sulfurospirillum-related sequences almost tripled from 1.3 % of the community before incubations to 3.7 and 4.2 % of the communities after incubation respectively without or with added 13 C-hexadecane (Table S2). The majority of the Geobacter sequences of this study were related to Fe III -reducing Geobacter psychrophilus strain P35 [56] and to Geobacter luticola, [57] whereas the Sulfurospirillum sequences of this study were related to As V reducing Sulfurospirillum deleyianum strain DSM 6946. [58] In addition, Geothrix-related sequences were enriched during incubations (Table S2), with the majority of them related to Fe III reducing Geothrix fermentans strain H5.…”

Section: Bacterial Communities Assessed By 16s Rrna Gene Amplicon Pyrmentioning

confidence: 88%

Microbially mediated reduction of FeIII and AsV in Cambodian sediments amended with 13C-labelled hexadecane and kerogen

et al. 2014

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Environmental context. The use of groundwater with elevated concentrations of arsenic for drinking, cooking or irrigation has resulted in the worst mass poisoning in human history. This study shows that organic compounds that can be found in arsenic rich subsurface sediments may be used by indigenous microorganisms, contributing to the release of arsenic from the sediments into the groundwater. This study increases our understanding of the range of organic substrates (and their sources) that can potentially stimulate arsenic mobilisation into groundwaters.Abstract. Microbial activity is generally accepted to play a critical role, with the aid of suitable organic carbon substrates, in the mobilisation of arsenic from sediments into shallow reducing groundwaters. The nature of the organic matter in natural aquifers driving the reduction of As V to As III is of particular importance but is poorly understood. In this study, sediments from an arsenic rich aquifer in Cambodia were amended with two 13 C-labelled organic substrates. 13C-hexadecane was used as a model for potentially bioavailable long chain n-alkanes and a 13 C-kerogen analogue as a proxy for non-extractable organic matter. During anaerobic incubation for 8 weeks, significant Fe III reduction and As III mobilisation were observed in the biotic microcosms only, suggesting that these processes were microbially driven. Microcosms amended with 13 C-hexadecane exhibited a similar extent of Fe III reduction to the non-amended microcosms, but marginally higher As III release. Moreover, gas chromatography-mass spectrometry analysis showed that 65 % of the added 13 C-hexadecane was degraded during the 8-week incubation. The degradation of 13 C-hexadecane was microbially driven, as confirmed by DNA stable isotope probing (DNA-SIP). Amendment with 13 C-kerogen did not enhance Fe III reduction or As III mobilisation, and microbial degradation of kerogen could not be confirmed conclusively by DNA-SIP fractionation or 13 C incorporation in the phospholipid fatty acids. These data are, therefore, consistent with the utilisation of long chain n-alkanes (but not kerogen) as electron donors for anaerobic processes, potentially including Fe III and As V reduction in the subsurface.

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Geobacter luticola sp. nov., an Fe(III)-reducing bacterium isolated from lotus field mud

Cited by 25 publications

References 34 publications

DNRA and Denitrification Coexist over a Broad Range of Acetate/N-NO3− Ratios, in a Chemostat Enrichment Culture

DNRA and Denitrification Coexist over a Broad Range of Acetate/N-NO3− Ratios, in a Chemostat Enrichment Culture

Geobacter soli sp. nov., a dissimilatory Fe(III)-reducing bacterium isolated from forest soil

Microbially mediated reduction of FeIII and AsV in Cambodian sediments amended with 13C-labelled hexadecane and kerogen

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