Inland water bodies and their surface hydrologic connections are active components in the landscape, influencing multiple ecological processes that can propagate to broadâscale phenomena such as regional nutrient and carbon cycles and metapopulation dynamics. However, while lake, wetland, and stream abundance has been estimated at regional and global extents, less attention has been paid to freshwater connectivity attributes among aquatic systems at macroscales. Thus, regional to continental patterns of freshwater abundance and connectivity are poorly understood. We measured lake, wetland, and stream abundance and surface connectivity attributes (i.e., landscape position within stream networks) at a subcontinental extent in the Midwest and Northeast United States to characterize macroscale spatial patterns of the freshwater landscape (i.e., abundance and connectivity attributes of lakes, wetlands, and streams). We found that lake and wetland abundance exhibited opposite spatial patterns from stream density that generally followed glaciation extent boundariesâlake and wetland abundance was high north of the glaciation boundary, whereas stream density was high south of the glaciation boundary. Freshwater connectivity attributes exhibited distinct spatial patterns as defined by our integrated freshwater clusters and revealed a layer of complexity not captured by abundance measures. Patterns of freshwater abundance and connectivity in the study extent were associated primarily with glaciation and secondarily with hydrogeomorphic (e.g., surficial geology and topography), climate (e.g., runoff), and landâuse (e.g., agriculture) variables, providing insight into potential drivers of freshwater composition and distribution. The connectivity spatial patterns observed suggest that relying solely on freshwater abundance measures in macroscale analyses omits unique information on the structural attributes of freshwater systems that can be critical to key ecological processes. Adopting an integrated freshwater landscape framework to study and manage freshwaters is essential as freshwater systems face broadâscale disturbances that may alter hydrologic connections and subsequently may impact ecosystem processes and services.