Germ‐line transformation of pink bollworm (Lepidoptera: Gelechiidae) mediated by the <i>piggyBac</i> transposable element

Peloquin, John J.; Thibault, Stephen T.; Staten, R. T.; Miller, Thomas A.

doi:10.1046/j.1365-2583.2000.00194.x

Cited by 125 publications

(96 citation statements)

References 42 publications

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“…Delivering nucleases at the ''one nucleus'' stage in the embryo offers several advantages over classical approaches used in lepidopterans, in which material is commonly delivered at the syncytial preblastoderm stage and at the posterior pole of the egg (Peloquin et al 2000;Tamura et al 2000;Marcus et al 2004;Takasu et al 2010), when many cleavage nuclei are present. One advantage of our delivery system is that it circumvents the need to define the location of germ cell precursors during embryonic development in species for which development remains poorly characterized.…”

Section: Discussionmentioning

confidence: 99%

Efficient targeted mutagenesis in the monarch butterfly using zinc-finger nucleases

et al. 2012

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The development of reverse-genetic tools in “nonmodel” insect species with distinct biology is critical to establish them as viable model systems. The eastern North American monarch butterfly (Danaus plexippus), whose genome is sequenced, has emerged as a model to study animal clocks, navigational mechanisms, and the genetic basis of long-distance migration. Here, we developed a highly efficient gene-targeting approach in the monarch using zinc-finger nucleases (ZFNs), engineered nucleases that generate mutations at targeted genomic sequences. We focused our ZFN approach on targeting the type 2 vertebrate-like cryptochrome gene of the monarch (designated cry2), which encodes a putative transcriptional repressor of the monarch circadian clockwork. Co-injections of mRNAs encoding ZFNs targeting the second exon of monarch cry2 into “one nucleus” stage embryos led to high-frequency nonhomologous end-joining-mediated, mutagenic lesions in the germline (up to 50%). Heritable ZFN-induced lesions in two independent lines produced truncated, nonfunctional CRY2 proteins, resulting in the in vivo disruption of circadian behavior and the molecular clock mechanism. Our work genetically defines CRY2 as an essential transcriptional repressor of the monarch circadian clock and provides a proof of concept for the use of ZFNs for manipulating genes in the monarch butterfly genome. Importantly, this approach could be used in other lepidopterans and “nonmodel” insects, thus opening new avenues to decipher the molecular underpinnings of a variety of biological processes.

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Section: Discussionmentioning

confidence: 99%

Efficient targeted mutagenesis in the monarch butterfly using zinc-finger nucleases

et al. 2012

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“…The rearing facility for this U.S. Department of Agriculture (USDA)-led program is based in Phoenix, Arizona, and can produce *200 million sterile moths per week. The first genetic transformation of pink bollworm was described in 2000 (Peloquin et al 2000). A number of transgenic strains have been developed subsequently.…”

Section: Safety Regulatory and Public Perception Issuesmentioning

confidence: 99%

Sterile-Insect Methods for Control of Mosquito-Borne Diseases: An Analysis

Alphey

Benedict

Bellini

et al. 2010

Vector-Borne and Zoonotic Diseases

477

442

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Effective vector control, and more specifically mosquito control, is a complex and difficult problem, as illustrated by the continuing prevalence (and spread) of mosquito-transmitted diseases. The sterile insect technique and similar methods control certain agricultural insect pest populations in a species-specific, environmentally sound, and effective manner; there is increased interest in applying this approach to vector control. Such an approach, like all others in use and development, is not a one-size-fits-all solution, and will be more appropriate in some situations than others. In addition, the proposed release of pest insects, and more so genetically modified pest insects, is bound to raise questions in the general public and the scientific community as to such a method's efficacy, safety, and sustainability. This article attempts to address these concerns and indicate where sterileinsect methods are likely to be useful for vector control.

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“…(3) Many insect species have had their full genomes sequenced (Weinstock et al 2006;Richards et al 2008; The International Aphid Genomics Consortium 2010; Werren et al 2010), making the identification of key components and targets of the DV patterning network more straightforward and efficient. (4) Crucially, techniques to test gene function-such as germline transformation (Berghammer et al 1999;Peloquin et al 2000;Tamura et al 2000;Pavlopoulos et al 2004;Nakamura et al 2010) and parental RNAi (pRNAi), where gene function can be knocked down in embryos by injecting dsRNA into pupal or adult females (Hughes and Kaufman 2000;Bucher et al 2002;Miyawaki et al 2004;Ohde et al 2009)-are widely applicable throughout the insects (see Fig. 1), which allows a deep understanding of gene interactions that is complementary to the opportunities for broad phylogenetic sampling of insect DV patterning mechanisms.…”

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confidence: 99%

The evolution of dorsal–ventral patterning mechanisms in insects

Lynch¹,

Roth²

2011

Genes Dev.

103

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The gene regulatory network (GRN) underpinning dorsal–ventral (DV) patterning of the Drosophila embryo is among the most thoroughly understood GRNs, making it an ideal system for comparative studies seeking to understand the evolution of development. With the emergence of widely applicable techniques for testing gene function, species with sequenced genomes, and multiple tractable species with diverse developmental modes, a phylogenetically broad and molecularly deep understanding of the evolution of DV axis formation in insects is feasible. Here, we review recent progress made in this field, compare our emerging molecular understanding to classical embryological experiments, and suggest future directions of inquiry.

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Germ‐line transformation of pink bollworm (Lepidoptera: Gelechiidae) mediated by the piggyBac transposable element

Cited by 125 publications

References 42 publications

Efficient targeted mutagenesis in the monarch butterfly using zinc-finger nucleases

Efficient targeted mutagenesis in the monarch butterfly using zinc-finger nucleases

Sterile-Insect Methods for Control of Mosquito-Borne Diseases: An Analysis

The evolution of dorsal–ventral patterning mechanisms in insects

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