Germination and the role of extracellular matrix in adhesion of urediniospores of Uromyces viciae-fabae to synthetic surfaces

Clement, John A.; Martin, Stephen G.; Porter, Robert P.; Butt, Tariq M.; Beckett, A.

doi:10.1016/s0953-7562(09)81182-9

Cited by 56 publications

(21 citation statements)

References 24 publications

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“…Hydrophobic interactions are known to be important in germ tube adhesion and appressorium formation by many fungal leaf pathogens, on both artificial surfaces and plant leaves (Clement et aL, 1993 ;Terhune & Hoch, 1993;Braun & Howard, 1994;Jelitto, Page & Read, 1994). Moreover, a close relationship between hydrophobicity and germling contact has been found, with poor appressorium production observed on 'waxless' leaves of host plants (Terhune & Hoch, 1993).…”

Section: Assessment Of Fungal Responses To Host-derived Signals Diffumentioning

confidence: 99%

Analysis of factors involved in fungal recognition responses to host‐derived signals by arbuscular mycorrhizal fungi

et al. 1996

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SUMMARYAn experimental model for the study of recognition responses by arbuscular mycorrhizal symbionts to host roots was validated by analysing the main factors which might affect such responses. Results showed that fungal responses to bost-derived signals were unaffected by the degree of sterility of the system, host age and mycorrhizal status, symbiont age, or different chemical and physical characteristics of hydrophilic membranes. Use of dialysis membranes showed that the molecules putatively eliciting fungal responses weigh less than 500 Da. Utilization of the model for studies of developmental expression of proteins and genes in arbuscular mycorrhizal symbionts is examined.

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Section: Assessment Of Fungal Responses To Host-derived Signals Diffumentioning

confidence: 99%

Analysis of factors involved in fungal recognition responses to host‐derived signals by arbuscular mycorrhizal fungi

et al. 1996

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“…Bacterial and fungal spores are a major contamination problem for the food, drug, and agricultural industries (1)(2)(3). Spores are propagative microbial vectors that can survive extremes of chemical and physical attack in harsh environments.…”

Section: Introductionmentioning

confidence: 99%

“…Spore adhesion is also cited as a target of fungal control in agriculture (1). Spore adhesion is a significant problem in the dairy industry, especially to pipelines where they may multiply and resporulate (5).…”

Section: Introductionmentioning

confidence: 99%

Direct Quantification of Aspergillus niger Spore Adhesion in Liquid Using an Atomic Force Microscope

Bowen

Lovitt

Wright

2000

Journal of Colloid and Interface Science

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“…Other fungi that produce an ECM or similar substances, which is also referred to as STM, are generally related to plant or nematode pathogens, including Magnaporthe grisea (33), Blumeria graminis (13,43), Pestalotia malicola (29), Cochliobolus heterostrophus (9), Uromyces viciae-fabae (15,21), Botrytis cinerea (16), Colletotrichum graminicola (42,43), Mycosphaerella graminicola (24), Bipolaris sorokiniana (6), and Drechmeria coniospora (35).…”

Section: Discussionmentioning

confidence: 99%

Production of an Extracellular Matrix as an Isotropic Growth Phase of Penicillium rubens on Gypsum

Bekker

Huinink

Adan

et al. 2012

Appl Environ Microbiol

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cIndoor mold represents an important environmental concern, but a fundamental knowledge of fungal growth stages is needed to limit indoor fungal proliferation on finishing materials used in buildings. The present study focused on the succession of germination stages of the common indoor fungus Penicillium rubens on a gypsum substrate. This substrate is used as a model system representing porous materials that are widely used in indoor environments. Imaging with cryo-scanning electron microscopy showed that the formation of an extracellular matrix (ECM) is a phase of the isotropic growth of P. rubens that is uniquely related to germinating conidia. Furthermore, the ECM is observed only when a dry-state inoculation of the surface is applied, i.e., applying conidia directly from a 7-day-old colony, mimicking airborne contamination of the surface. When inoculation is done by spraying an aqueous conidial suspension, no ECM is observed. Moreover, it is concluded that the formation of an ECM requires active processes in the fungal cell. The porosity of the substrate proved that the ECM substance has high-viscosity characteristics. The present results stress that studies of indoor fungal growth should consider the method of inoculation, knowing that the common aqueous suspension may obscure specific stages in the initial phases of germination. Indoor mold and moisture and their associated health effects are widespread and important societal problems (11,27). Without doubt, mold growth requires the presence of water. Water can occur in different forms and may originate from different sources in the indoor environment, i.e., as water vapor in the indoor airair humidity-or in liquid form in the pore system of a material, originating from external sources such as rain, melting snow, rising dampness, or accidental leakage.Detailed understanding of the fungal growth phases and the interaction of a substrate, water, and fungi provides information to identify the weakest link in fungal growth. Such knowledge lays the foundation for effective intervention or prevention strategies. The availability of water can be expressed in many ways. Common parameters are water activity (a w ), which is widely used in microbiology to describe the water in substrates, and relative humidity (RH), which is typically used to describe water vapor in air. In equilibrium, the two quantities are directly related (26,39).Indoor fungi can be grouped according to their water tolerance. Magan and Lacey (38) distinguished hydrophilic (waterloving) and xerophilic (dry-loving) species on the basis of the minimum water activity required for growth. This scheme has been widely accepted and is based on a 0.9 a w or 90% RH threshold value.It is known that a w or RH influences each of the main phases of the growth cycle. In studies of Aspergillus and Penicillium species (1, 7, 41) on culture media, the minimum value of the a w for germination was lower than that for linear growth, which in turn was usually lower than that for asexual sporulation.Many studies hav...

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Germination and the role of extracellular matrix in adhesion of urediniospores of Uromyces viciae-fabae to synthetic surfaces

Cited by 56 publications

References 24 publications

Analysis of factors involved in fungal recognition responses to host‐derived signals by arbuscular mycorrhizal fungi

Analysis of factors involved in fungal recognition responses to host‐derived signals by arbuscular mycorrhizal fungi

Direct Quantification of Aspergillus niger Spore Adhesion in Liquid Using an Atomic Force Microscope

Production of an Extracellular Matrix as an Isotropic Growth Phase of Penicillium rubens on Gypsum

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