Giant Gold Nanowire Vesicle-Based Colorimetric and SERS Dual-Mode Immunosensor for Ultrasensitive Detection of Vibrio parahemolyticus

Abstract

Conventional methods for the detection of Vibrio parahemolyticus (VP) usually need tedious, labor-intensive processes, and have low sensitivity, which further limits their practical applications. Herein, we developed a simple and efficient colorimetry and surface-enhanced Raman scattering (SERS) dual-mode immunosensor for sensitive detection of VP, by employing giant Au vesicles with anchored tiny gold nanowires (AuNW) as a smart probe. Due to the larger specific surface and special hollow structure of giant Au vesicles, silver staining would easily lead to vivid color change for colorimetric analysis and further amplify SERS signals. The t-test was further used to determine if two sets of data from colorimetry and SERS were significantly different from each other. The result shows that there was no significant difference between data from the two methods. Two sets of data can mutually validate each other and avoid false positive and negative detection. The designed colorimetry-SERS dual-mode sensor would be very promising in various applications such as food safety inspection, personal healthcare, and on-site environmental monitoring.

Nonspherical Metal‐Based Nanoarchitectures: Synthesis and Impact of Size, Shape, and Composition on Their Biological Activity

“…The use of 4‐mercaptobenzoic acid prevents circumferential growth at the active site; asymmetric growth of the nanocrystals produces the nanowires. [ 129–131 ]…”

Molecular Architectonics‐guided Design of Biomaterials

“…The use of CDP as auxiliary in the scheme of molecular architecture to develop a novel AIEgen based smart systems for biosensing applications was demonstrated. Chen et al., developed silver strained Au vesicles for the detection of Vibrio parahemolyticus based on SERS and colorimetric methods [35] …”

Detection of Aflatoxin B1 Based on a Porous Anodized Aluminum Membrane Combined with Surface-Enhanced Raman Scattering Spectroscopy

“…Therefore, it is necessary to develop a reliable, convenient method for detecting AFB 1 Surface-enhanced Raman scattering (SERS) has been applied in many fields [14], such as food chemistry [15][16][17], environmental science [18], and biotechnology [19][20][21]. At present, there are many Surface-enhanced Raman scattering (SERS) has been applied in many fields [14], such as food chemistry [15][16][17], environmental science [18], and biotechnology [19][20][21]. At present, there are many reports about detection of AFB1 via SERS technology [22][23][24][25][26].…”