Gibberellic acid overproduction in Fusarium fujikuroi using regulatory modification and transcription analysis

Wang, Haonan; Ke, Xia; Jia, Rui; Huang, Lianggang; Liu, Zhi‐Qiang; Zheng, Yu‐Guo

doi:10.1007/s00253-023-12498-0

Cited by 3 publications

(1 citation statement)

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“…For instance, Wang and co-workers (2022) suggested a multivariate strategy of metabolic engineering, where three modules from the GA 3 biosynthetic pathway consisting of a precursor pool, a cluster-specific channel, and a P450-mediated oxidation were optimized to achieve a reconstitution of the metabolic balance of the industrial GA 3 producer strain, which showed an increased production of 48.2% [ 89 ]. Likewise, the most recent report presents a regulatory modification strategy based on the overexpression of AreA and Lae1, two regulators that positively influence GA 3 biosynthesis [ 90 ]. From this work, the third-highest production rate was achieved, of 431 mg/L/day, by coupling promoter engineering, metabolic modification, and transcriptome analysis in the strain Fusarium fujikuroi CGMCC No: M 2,019,378.…”

Section: Ga 3 Production Improvement By Genetic En...mentioning

confidence: 99%

Bioprocess of Gibberellic Acid by Fusarium fujikuroi: The Challenge of Regulation, Raw Materials, and Product Yields

Hernández Rodríguez,

Díaz Pacheco,

Martínez Tolibia

et al. 2024

JoF

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Gibberellic acid (GA3) is a tetracyclic diterpenoid carboxylic acid synthesized by the secondary metabolism of Fusarium fujikuroi. This phytohormone is widely studied due to the advantages it offers as a plant growth regulator, such as growth stimulation, senescence delay, flowering induction, increased fruit size, and defense against abiotic or biotic stress, which improve the quality and yield of crops. Therefore, GA3 has been considered as an innovative strategy to improve agricultural production. However, the yields obtained at large scale are insufficient for the current market demand. This low productivity is attributed to the lack of adequate parameters to optimize the fermentation process, as well as the complexity of its regulation. Therefore, this article describes the latest advances for potentializing the GA3 production process, including an analysis of its origins from crops, the benefits of its application, the related biosynthetic metabolism, the maximum yields achieved from production processes, and their association with genetic engineering techniques for GA3 producers. This work provides a new perspective on the critical points of the production process, in order to overcome the limits surrounding this modern line of bioengineering.

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Section: Ga 3 Production Improvement By Genetic En...mentioning

confidence: 99%