The transport of moisture in the tropics is a critical process for the global energy budget and on geologic timescales, has markedly influenced continental landscapes, migratory pathways, and biological evolution. Here we present a continuous, first-of-its-kind 1.3-My record of continental hydroclimate and lake-level variability derived from drill core data from Lake Malawi, East Africa (9-15°S). Over the Quaternary, we observe dramatic shifts in effective moisture, resulting in large-scale changes in one of the world's largest lakes and most diverse freshwater ecosystems. Results show evidence for 24 lake level drops of more than 200 m during the Late Quaternary, including 15 lowstands when water levels were more than 400 m lower than modern. A dramatic shift is observed at the Mid-Pleistocene Transition (MPT), consistent with far-field climate forcing, which separates vastly different hydroclimate regimes before and after ∼800,000 years ago. Before 800 ka, lake levels were lower, indicating a climate drier than today, and water levels changed frequently. Following the MPT high-amplitude lake level variations dominate the record. From 800 to 100 ka, a deep, often overfilled lake occupied the basin, indicating a wetter climate, but these highstands were interrupted by prolonged intervals of extreme drought. Periods of high lake level are observed during times of high eccentricity. The extreme hydroclimate variability exerted a profound influence on the Lake Malawi endemic cichlid fish species flock; the geographically extensive habitat reconfiguration provided novel ecological opportunities, enabling new populations to differentiate rapidly to distinct species. Lake Malawi | tropical paleoclimatology | East African rift | cichlid fish | quaternary I nsolation forcing of tropical convection and shifts in the Intertropical Convergence Zone (ITCZ) are considered principal mechanisms driving tropical climate variability on geologic time scales (1-5). However, instrumental records show that the ITCZ over land is poorly characterized compared with the oceans (Fig. 1), and the transport of oceanic moisture to the hinterlands is complex, because topographic barriers redirect winds and block advection to the continental interiors (6). To document moisture transport onto the continents over geological time scales, terrestrial records of tropical paleoclimate with the length and continuity of ocean drilling records are required. Continental drilling in long-lived tropical lakes provides continuous, high-resolution paleoclimate records that extend well past the last glacial maximum (7,8), and augment shorter, well-dated records from outcrops. Lakes of great antiquity also contain numerous endemic species-notably, cichlid fishes-whose modern assemblages evolved in concert with changing climates, and which figure prominently into models of speciation and diversification (9, 10).Lake Malawi and Its Catchment Lake Malawi (Nyasa) is one of the world's largest and oldest lakes, and is situated at the southern end of the East Afri...