Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites

Uzelac, Branka; Stojičić, Dragana; Budimir, Snežana

doi:10.1007/978-3-030-30185-9_1

Cited by 7 publications

(6 citation statements)

References 143 publications

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“…In lieu of a reliable cannabis transformation protocol, we used a heterologous system to determine if there is a causal effect of CsMIXTA expression on trichome morphogenesis. Tobacco ( Nicotiana tabacum) , like cannabis, has glandular stalked trichomes but in the case of tobacco, GSTs are abundant on the leaves [ 19 ]. We ectopically expressed CsMIXTA (controlled by a constitutive (35S CaMV) promoter) in tobacco plants to evaluate if this cannabis TF could regulate glandular trichome initiation/development on tobacco leaves.…”

Section: Resultsmentioning

confidence: 99%

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Overexpression of CsMIXTA, a Transcription Factor from Cannabis sativa, Increases Glandular Trichome Density in Tobacco Leaves

Haiden

Apicella

et al. 2022

Plants

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Cannabinoids are synthesized in glandular stalked trichomes on the female flowers of Cannabis sativa (cannabis). The regulation of glandular trichome development has not been characterized in cannabis. We recently identified an R2R3-MYB transcription factor, CsMIXTA, which could be involved in trichome morphogenesis in cannabis. Some homologous genes of CsMIXTA are known to function in glandular trichome initiation in other plant species. CsMIXTA is highly expressed in flower tissue compared to vegetative tissues. Interestingly, CsMIXTA is also highly expressed in trichomes isolated from female flower tissue. In addition, CsMIXTA is upregulated during the peak stages of female flower maturation in correlation with some cannabinoid biosynthetic genes. Transient expression in Nicotiana benthamiana showed that CsMIXTA is localized in the nucleus. Furthermore, yeast transcriptional activation assay demonstrated that CsMIXTA has transactivation activity. Overexpression of CsMIXTA in Nicotiana tabacum resulted in higher trichome density, larger trichome size, and more branching on stalked glandular trichomes. The results indicate that CsMIXTA not only promotes glandular trichome initiation in epidermal cells, but also regulates trichome development in tobacco leaves. In this report, we characterized the novel function of the first cannabis transcription factor that may be critical for glandular trichome morphogenesis.

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Section: Resultsmentioning

confidence: 99%

“…Both lines ( Figure 4 B,C) showed substantially higher trichome density compared to wt ( Figure 4 A). Typical wt trichomes possess only one gland and rarely branch [ 19 ]. However, we observed highly branched glandular stalked trichomes in both transgenic lines.…”

Section: Resultsmentioning

confidence: 99%

Overexpression of CsMIXTA, a Transcription Factor from Cannabis sativa, Increases Glandular Trichome Density in Tobacco Leaves

Haiden

Apicella

et al. 2022

Plants

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“…Nicotiana tabacum L. (the flue-cured tobacco), an herbaceous plant belonging to the Nicotiana genus (Solanaceae family), is now cultivated worldwide and has long been used medicinally and recreationally as the most economically important industrial crops[1,2]. Moreover, Nicotiana species are commonly investigated for the biological activity of their specialized metabolites in responses to abiotic stress or biotic stress factors such as pathogens[3,4].…”

Section: Introductionmentioning

confidence: 99%

Extraction, purification and anti-TMV effects of α (β)-2,7,11-cembratriene-4,6-diol from tobacco leaves

Feng

Jia

Guan

et al. 2021

Preprint

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Background Acetone ethanol extracts from tobacco leaves have antiviral activity against TMV, but the antiviral effects of their specialized metabolites have not been systematically studied yet, especially the underlying mechanism is still unexplored. Results The tobacco cembranoids α(β)-2,7,11-cembratriene-4,6-diol (α(β)-CBD) were extracted and purified with an effective and green protocol including innovatively added 5% phosphoric acid for elution, one time silica gel chromatographic column separation and impurity removal and further HPLC purification. The results of antiviral activities against tobacco mosaic virus (TMV) with the local lesion counting method showed that α(β)-CBD have in vivo higher protective effects of 73.2% and 71.6%, at 75.0 μM, respectively, than control agent Ningnanmycin (53.1%). Notably, The results of ELISA and and TMV-GFP fluorescent optical imaging assay indicated a obviously reduced viral protein and weaker GFP fluorescence signal and smaller infection area, which confirmed their anti-TMV activities at protein level. Furthermore, the enhanced production of SA and JA and the significantly increased transcription of of JA signaling pathway (COI1 and PDF1.2) and SA signaling pathway genes (PR1, NPR1 and EDS1) in treated plants further conformed that exogenously applied α(β)-CBD can effectively elicit the tobacco plant immunity against TMV. Conclusions The α(β)-CBD mainly stimulates disease resistance of tobacco plants to resist TMV and it can be used as bioagents to control TMV in the future.

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Section: Introductionmentioning

confidence: 99%

Extraction, purification and anti-TMV effects of α (β)-2,7,11-cembratriene-4,6-diol from tobacco leaves

Yan

Jia

Wei

et al. 2021

Industrial Crops and Products

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Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites

Cited by 7 publications

References 143 publications

Overexpression of CsMIXTA, a Transcription Factor from Cannabis sativa, Increases Glandular Trichome Density in Tobacco Leaves

Overexpression of CsMIXTA, a Transcription Factor from Cannabis sativa, Increases Glandular Trichome Density in Tobacco Leaves

Extraction, purification and anti-TMV effects of α (β)-2,7,11-cembratriene-4,6-diol from tobacco leaves

Extraction, purification and anti-TMV effects of α (β)-2,7,11-cembratriene-4,6-diol from tobacco leaves

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