2014
DOI: 10.1016/j.celrep.2014.10.065
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Global Proteome Turnover Analyses of the Yeasts S. cerevisiae and S. pombe

Abstract: How cells maintain specific levels of each protein and whether that control is evolutionarily conserved are key questions. Here, we report proteome-wide steady-state protein turnover rate measurements for the evolutionarily distant, but ecologically similar yeasts, Saccharomyces cerevisiae and Schizosaccharomyces pombe. We find that the half-lives of most proteins is much longer than currently thought and determined to a large degree by proteins synthesis and dilution due to cell division. However, we detect a… Show more

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Cited by 261 publications
(388 citation statements)
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“…We found values for mean mRNA levels (m), mean protein levels (p), mRNA decay rates (d 2 ) and protein decay rates (d 4 ) for 2390 different unique genes from experimental papers (Marguerat et al, 2012;Amorim et al, 2010;Christiano et al, 2014). These parameters and the correlations between them are displayed in Figure 7.…”
Section: Schizosaccharomyces Pombe Parameter Studymentioning
confidence: 99%
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“…We found values for mean mRNA levels (m), mean protein levels (p), mRNA decay rates (d 2 ) and protein decay rates (d 4 ) for 2390 different unique genes from experimental papers (Marguerat et al, 2012;Amorim et al, 2010;Christiano et al, 2014). These parameters and the correlations between them are displayed in Figure 7.…”
Section: Schizosaccharomyces Pombe Parameter Studymentioning
confidence: 99%
“…It is clear from this figure that there exists a strong correlation between mean mRNA and protein levels and weak correlation between the other parameters. (Marguerat et al, 2012;Amorim et al, 2010;Christiano et al, 2014). Histograms are shown on diagonal panels and scatter plots comparing parameters are shown on off-diagonal panels.…”
Section: Schizosaccharomyces Pombe Parameter Studymentioning
confidence: 99%
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“…In metabolic engineering, specific protein levels are typically controlled through transcription (at the promoter level); control mechanisms at other levels, especially at the protein level, have not been well exploited in yeast. Protein degradation is a natural mechansim widely used in many cellular/metabolic regulation processes, including cell cycling (Sandoval et al, 2013) and sterol homeostasis (Christiano et al, 2014;Foresti et al, 2013;Meusser et al, 2005). In S. cerevisiae, the squalene-consuming enzyme squalene epoxidase (Erg1p; Fig.…”
Section: Introductionmentioning
confidence: 99%
“…In S. cerevisiae, the squalene-consuming enzyme squalene epoxidase (Erg1p; Fig. 1) has a short half-life (60-80 min) due to protein degradation, whereas squalene synthase (Erg9p) is more stable, with a half life of 10 hours (Christiano et al, 2014;Foresti et al, 2013;Ruckenstuhl et al, 2007). The short half-life of Erg1p provides a mechanism for the cell to rapidly shut down squalene consumption when high flux is not required for downstream synthesis (Foresti et al, 2013).…”
Section: Introductionmentioning
confidence: 99%