In this study, we evaluate uncertainties propagated through different climate data sets in seasonal and annual hydrological simulations over 10 subarctic watersheds of northern Manitoba, Canada, using the variable infiltration capacity (VIC) model. Further, we perform a comprehensive sensitivity and uncertainty analysis of the VIC model using a robust and state-of-the-art approach. The VIC model simulations utilize the recently developed variogram analysis of response surfaces (VARS) technique that requires in this application more than 6,000 model simulations for a 30-year (1981-2010) study period. The method seeks parameter sensitivity, identifies influential parameters, and showcases streamflow sensitivity to parameter uncertainty at seasonal and annual timescales. Results suggest that the Ensemble VIC simulations match observed streamflow closest, whereas global reanalysis products yield high flows (0.5-3.0 mm day −1 ) against observations and an overestimation (10-60%) in seasonal and annual water balance terms. VIC parameters exhibit seasonal importance in VARS, and the choice of input data and performance metrics substantially affect sensitivity analysis. Uncertainty propagation due to input forcing selection in each water balance term (i.e., total runoff, soil moisture, and evapotranspiration) is examined separately to show both time and space dimensionality in available forcing data at seasonal and annual timescales. Reliable input forcing, the most influential model parameters, and the uncertainty envelope in streamflow prediction are presented for the VIC model. These results, along with some specific recommendations, are expected to assist the broader VIC modelling community and other users of VARS and land surface schemes, to enhance their modelling applications. K E Y W O R D S hydrological modelling, lower Nelson River basin, sensitivity analysis, uncertainty assessment, VARS, VIC model, VIC parameters, water balance 1 | INTRODUCTION Numerical modelling of a river basin remains essential for both climate and ecological studies as it provides vital information about the hydrological cycle and water availability for societies and ecosystems. Although recent developments and advances have been achieved in hydrological modelling and computational power, addressing efficiently the uncertainties in hydrological simulation remains a critical