Global Transcriptome Analysis and Identification of Differentially Expressed Genes after Infection of Fragaria vesca with Powdery Mildew (Podosphaera aphanis)

Jambagi, Shridhar; Dunwell, Jim M.

doi:10.4172/2329-8936.1000106

Cited by 16 publications

(12 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the KEGG pathways identified in two groups, the flavanol biosynthesis pathway was the most enriched, indicating the importance of gene regulation of the flavanol metabolite in octoploid strawberry in response to P. aphanis. Previous work has shown that genes related to secondary metabolism, signal transduction, transcription factors and disease resistance play important role in defense against P. aphanis [18].…”

Section: Distinctive Phenotypes Of the Two Strawberry Groupsmentioning

confidence: 99%

“…Despite the availability of total genome sequence information for octoploid strawberry [17], its mechanisms of defence against P. aphanis at the molecular level remain to be clarified. Transcriptome analysis has shown differentially expressed genes (DEGs) related to secondary metabolism, signal transduction, and disease resistance were upregulated and played crucial roles in the early defence against P. aphanis [18]. Furthermore, functional identification of candidate genes from the diploid strawberry has enabled the investigation of resistance to P. aphanis, including FvHsfB1a [19], FvMLO [20], and FvWRKY42 [21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Salicylic acid-primed defence response in octoploid strawberry ‘Benihoppe’ leaves induces resistance against Podosphaera aphanis through enhanced accumulation of proanthocyanidins and upregulation of pathogenesis-related genes

et al. 2020

View full text Add to dashboard Cite

Background: Podosphaera aphanis, a predominately biotrophic fungal pathogen, causes significant yield losses of strawberry. China is the largest strawberry producer in the world, and selecting for powdery mildew-resistant cultivars is desirable. However, the resistance mechanism against P. aphanis in the octoploid strawberry remains unclear.Results: To understand possible mechanisms of disease resistance, we inoculated strawberry leaves with P. aphanis, and examined the expression profiles of candidate genes and the biochemical phenotypes in strawberry leaves of two groups. The unigenes obtained from ddH 2 O-and SA-pretreated leaves resulted in a total of 48,020 and 45,896 genes, respectively. KEGG enrichment showed that phenylpropanoid biosynthesis and plant hormone signal transduction pathways were enriched to a noticeable extent. DEG analysis showed that key TFs genes associated with the SA signaling pathway could play important role in the strawberry-P. aphanis interaction. In particular, FaWRKY70, FaJAZ1 and FaMYC2-like, involved in regulating the antagonistic effect of SA and JA signaling pathway, leading to increased expression of SA-responsive genes (in particular PR1, PR2, PR3, and PR5) compared to a decline in expression of JA-responsive genes (FaJAR1, FaAOS, and FaLOX2). Furthermore, SA pretreatment induced accumulation of PAs by activating the MBW complex and inhibit powdery mildew growth. Conclusions: This study describes the role of the proanthocyanidins (PAs), pathogenesis-related (PR) genes, SA, and transcription factors in regulatory model against P. aphanis, which coincided with an early activation of defense, leading to the accumulation of PAs and the PR proteins.

show abstract

Section: Distinctive Phenotypes Of the Two Strawberry Groupsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Salicylic acid-primed defence response in octoploid strawberry ‘Benihoppe’ leaves induces resistance against Podosphaera aphanis through enhanced accumulation of proanthocyanidins and upregulation of pathogenesis-related genes

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Here, Fragaria vesca, Fragaria × ananassa, and Malus × domestica (only these three species in the present study can be found the available RNA-seq data to analyze the expression profiles about diverse tissues and stresses) were considered when investigating the RALF-like gene expression of diverse tissues, organs, developmental stages, and stresses. The study accessions from NCBI were SRP096282, SRP098567, ERP004230, SRP035308, SRP018410, ERP013896, SRP111905, SRP125281, SRP091754, and SRP139480 [54][55][56][57][58][59][60][61], which were involved in the development of flower tissues, fruit tissues, seed tissues, and fungal stresses. Ultimately, the FPKM (fragments per kilobase per million) [62] of RALF-like genes was extracted from the GTF files and submitted to TBtools [52] to draw the heatmaps with the log 2 translation of FPKM.…”

Section: The Expression Profiles Of Ralf-like Genes In Strawberry Andmentioning

confidence: 99%

The Genome-Wide Analysis of RALF-Like Genes in Strawberry (Wild and Cultivated) and Five Other Plant Species (Rosaceae)

Zhang

Jing

Chen

et al. 2020

Genes

View full text Add to dashboard Cite

The rapid alkalinization factor (RALF) gene family is essential for the plant growth and development. However, there is little known about these genes among Rosaceae species. Here, we identify 124 RALF-like genes from seven Rosaceae species, and 39 genes from Arabidopsis, totally 163 genes, divided into four clades according to the phylogenetic analysis, which includes 45 mature RALF genes from Rosaceae species. The YISY motif and RRXL cleavage site are typical features of true RALF genes, but some variants were detected in our study, such as YISP, YIST, NISY, YINY, YIGY, YVGY, FIGY, YIAY, and RRVM. Motif1 is widely distributed among all the clades. According to screening of cis-regulatory elements, GO annotation, expression sequence tags (EST), RNA-seq, and RT-qPCR, we reported that 24 RALF genes coding mature proteins related to tissue development, fungal infection, and hormone response. Purifying selection may play an important role in the evolutionary process of RALF-like genes among Rosaceae species according to the result from ka/ks. The tandem duplication event just occurs in four gene pairs (Fv-RALF9 and Fv-RALF10, Md-RALF7 and Md-RALF8, Pm-RALF2 and Pm-RALF8, and Pp-RALF11 and Pp-RALF14) from four Rosaceae species. Our research provides a wide overview of RALF-like genes in seven Rosaceae species involved in identification, classification, structure, expression, and evolution analysis.

show abstract

“…In particular, PR10 genes seem to play a central role in the response of the Rosaceae. Studies of the Rosaceae model plant Fragaria infected by pathogens with different lifestyles, such as the necrotrophic fungus Botrytis cinerea (González et al 2013), the obligate biotrophic powdery mildew fungus Podosphaera aphanis (Jambagi and Dunwell 2015) and the hemibiotrophic oomycete Phytophthora cactorum (Toljamo et al 2016), showed a strong upregulation of PR10 genes or major allergens. Studies on apple (Malus) infected with Venturia inaequalis (apple scab) (Poupard et al 2003;Cova et al 2017) and treated with fungal elicitors (Pühringer et al 2000) also showed an upregulation of the PR10 genes.…”

Section: Common Response To Both Pathogensmentioning

confidence: 99%

Interaction of roses with a biotrophic and a hemibiotrophic leaf pathogen leads to differences in defense transcriptome activation

et al. 2019

View full text Add to dashboard Cite

Key message Transcriptomic analysis resulted in the upregulation of the genes related to common defense mechanisms for black spot and the downregulation of the genes related to photosynthesis and cell wall modification for powdery mildew. Abstract Plant pathogenic fungi successfully colonize their hosts by manipulating the host defense mechanisms, which is accompanied by major transcriptome changes in the host. To characterize compatible plant pathogen interactions at early stages of infection by the obligate biotrophic fungus Podosphaera pannosa, which causes powdery mildew, and the hemibiotrophic fungus Diplocarpon rosae, which causes black spot, we analyzed changes in the leaf transcriptome after the inoculation of detached rose leaves with each pathogen. In addition, we analyzed differences in the transcriptomic changes inflicted by both pathogens as a first step to characterize specific infection strategies. Transcriptomic changes were analyzed using next-generation sequencing based on the massive analysis of cDNA ends approach, which was validated using high-throughput qPCR. We identified a large number of differentially regulated genes. A common set of the differentially regulated genes comprised of pathogenesis-related (PR) genes, such as of PR10 homologs, chitinases and defense-related transcription factors, such as various WRKY genes, indicating a conserved but insufficient PTI [pathogen associated molecular pattern (PAMP) triggered immunity] reaction. Surprisingly, most of the differentially regulated genes were specific to the interactions with either P. pannosa or D. rosae. Specific regulation in response to D. rosae was detected for genes from the phenylpropanoid and flavonoid pathways and for individual PR genes, such as paralogs of PR1 and PR5, and other factors of the salicylic acid signaling pathway. Differently, inoculation with P. pannosa leads in addition to the general pathogen response to a downregulation of genes related to photosynthesis and cell wall modification.Keywords Black spot · Powdery mildew · MACE analysis · High-throughput qPCR · WRKY genes · PR genes Enzo Neu and Helena Sophia Domes have contributed equally to this work. Electronic supplementary materialThe online version of this article (https ://doi.

show abstract

Global Transcriptome Analysis and Identification of Differentially Expressed Genes after Infection of Fragaria vesca with Powdery Mildew (Podosphaera aphanis)

Cited by 16 publications

References 51 publications

Salicylic acid-primed defence response in octoploid strawberry ‘Benihoppe’ leaves induces resistance against Podosphaera aphanis through enhanced accumulation of proanthocyanidins and upregulation of pathogenesis-related genes

Salicylic acid-primed defence response in octoploid strawberry ‘Benihoppe’ leaves induces resistance against Podosphaera aphanis through enhanced accumulation of proanthocyanidins and upregulation of pathogenesis-related genes

The Genome-Wide Analysis of RALF-Like Genes in Strawberry (Wild and Cultivated) and Five Other Plant Species (Rosaceae)

Interaction of roses with a biotrophic and a hemibiotrophic leaf pathogen leads to differences in defense transcriptome activation

Contact Info

Product

Resources

About