Glucagon-like peptide-1 production in the GLUTag cell line is impaired by free fatty acids via endoplasmic reticulum stress

Hayashi, Hiroto; Yamada, Ryoichi; Das, Siddhartha; Sato, Taiki; Takahashi, Aki; Hiratsuka, Masahiro; Hirasawa, Noriyasu

doi:10.1016/j.metabol.2014.02.012

Cited by 37 publications

(38 citation statements)

References 54 publications

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“…In HFD pups, however, the levels of these peptides did not change relative to those in C pups. Recently Hayashi et al [18] reported that GLP-1 production in the GLUTag cell line is impaired by free fatty acids via endoplasmic reticulum stress and a reduction in the protein levels of prohormone convertase 1/3, suggesting that the discrepancy between mRNA expression and production of GLP-1 might happen in HFD pups. The differences in production of incretins between the OF and HFD pups may relate to the differences in insulin secretion with insulin resistance.…”

Section: Discussionmentioning

confidence: 99%

Postnatal weight gain induced by overfeeding pups and maternal high-fat diet during the lactation period modulates glucose metabolism and the production of pancreatic and gastrointestinal peptides

Hosoda

Umekawa

et al. 2015

Peptides

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Section: Discussionmentioning

confidence: 99%

Postnatal weight gain induced by overfeeding pups and maternal high-fat diet during the lactation period modulates glucose metabolism and the production of pancreatic and gastrointestinal peptides

Hosoda

Umekawa

et al. 2015

Peptides

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“…However, accumulated evidence has proved the crucial effect of FFAs in the pathogenesis of atherosclerotic diseases. Excess of serum FFAs can induce reactive oxygen species production,5 endoplasmic reticulum stress,6 apoptosis,7 inflammation and thrombosis 8. Finally, all these disadvantageous impacts may result in impaired cardiovascular functions.…”

Section: Discussionmentioning

confidence: 99%

Free fatty acids as a marker for predicting periprocedural myocardial injury after coronary intervention

Wang

Zhang

Guo

et al. 2019

Postgraduate Medical Journal

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BackgroundPrevious studies have revealed that plasma levels of free fatty acids (FFAs) are related to cardiovascular risk. However, whether FFAs could predict periprocedural myocardial injury (PMI) following percutaneous coronary intervention (PCI) in patients with stable coronary artery disease (CAD) remains unclear.PurposeThis study aimed to investigate the relationship of FFAs to PMI in untreated patients with CAD who underwent PCI.MethodsA total of 374 consecutive patients with CAD without lipid-lowering treatment on admission and with normal preprocedural cardiac troponin I (cTnI) levels who underwent PCI were prospectively enrolled. The baseline characteristics were collected and PMI was evaluated by cTnI analysis within 24 hours. The relation of preprocedural FFA levels to peak cTnI values after PCI was examined.ResultsPreprocedural FFAs were positively correlated with peak cTnI values after PCI in both simple regression model (β=0.119, p=0.021) and multiple regression model (β=0.198, p=0.001). Patients with higher FFA levels had higher postprocedural cTnI levels compared with those with normal FFA levels (0.27±0.68 ng/mL vs 0.66±0.31 ng/mL, p=0.014). In the multivariable model, preprocedural FFA levels were associated with an increased risk of postprocedural cTnI elevation above 1× upper limit of normal (ULN, OR: 1.185, 95% CI 0.997 to 1.223, p=0.019) up to 10× ULN (OR: 1.132, 95% CI 1.005 to 1.192, p=0.003) .ConclusionsThe present study first suggested that elevated FFA levels were associated with an increased risk of PMI in untreated patients with CAD. Further study with large sample size may be needed to confirm our findings.

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“…Hence, we drew the conclusion that blocking AT1R expression by GLP-1 was responsible for the anti-hypertensive effects. The intake of abundant saturated fatty acids resulted in ERS in the intestines and decreased GLP-1 production [25], suggesting that exogenous GLP-1 may reduce the injury. GLP-1 receptor analog remarkably decreased markers of inflammation, oxidative stress, and fibrosis in the kidneys of maternal obesity mice [26].…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Glucagon-Like Peptide-1 Analog on Renal Tubular Injury in Mice on High-Fat Diet

Guo

Wang

et al. 2017

Cell Physiol Biochem

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Aims: The study aimed to investigate the renoprotective effect of glucagon-like peptide-1 (GLP-1) against renal tubular injury in C57BL/6 mice induced by a high-fat diet (HFD). Methods: Twenty C57BL/6 mice were fed HFD for 12 weeks. Ten of these mice were treated with GLP-1 at 200 µg/kg subcutaneously twice daily for 4 weeks (HFDG group), and the other ten mice received vehicle only (HFD group). Ten mice fed standard rodent chow served as controls (Con group). Body weight, kidney weight, food intake, and systolic blood pressure were measured. The expression of endoplasmic reticulum stress (ERS) markers (BIP, p-eIF2α, ATF4, and CHOP) and apoptosis in the kidney were examined utilizing western blotting, immunohistochemistry and TUNEL, respectively. Angiotensin II and angiotensin II type 1 receptor (AT1R) were examined by ELISA. Human proximal tubule epithelial cells (HK2) were treated with GLP-1(150 nM) followed by treatment with palmitic acid (500 nM [PA]) for 24 h. HK2 cells treated with BSA were used as controls. The protein levels of ERS markers, apoptosis-associated protein, and AT1R were measured by western blotting. Results: Increase of body weight, food intake, and systolic blood pressure was less pronounced in GLP-1 treated HFDG mice compared to HFD mice. The levels of ERS markers (BIP, p-eIF2α, ATF4, and CHOP) and apoptosis decreased following GLP-1 treatment in vivo and in vitro (p<0.05). Increased AT1R induced by HFD and PA were blocked with GLP-1 treatment. In contrast, the level of angiotensin II after GLP-1 treatment was not significantly different between the HFD and HFDG mice. Conclusion: The study indicated that saturated fatty acids induced ERS and apoptosis in the kidney and increased AT1R expression. GLP-1 treatment exerted renoprotective effects against saturated fatty acid-induced kidney tubular cell ERS and apoptosis together with inhibition of AT1R expression in vivo and in vitro.

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Glucagon-like peptide-1 production in the GLUTag cell line is impaired by free fatty acids via endoplasmic reticulum stress

Cited by 37 publications

References 54 publications

Postnatal weight gain induced by overfeeding pups and maternal high-fat diet during the lactation period modulates glucose metabolism and the production of pancreatic and gastrointestinal peptides

Postnatal weight gain induced by overfeeding pups and maternal high-fat diet during the lactation period modulates glucose metabolism and the production of pancreatic and gastrointestinal peptides

Free fatty acids as a marker for predicting periprocedural myocardial injury after coronary intervention

Protective Effects of Glucagon-Like Peptide-1 Analog on Renal Tubular Injury in Mice on High-Fat Diet

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