Glucose-regulated protein 78 is an antiviral against hepatitis A virus replication

Jiang, Xia; Haga, Yuki; Sasaki, Reina; Nakamura, Masato; Wu, Shuang; Nakamoto, Shingo; Shirasawa, Hiroshi; Okamoto, Hiroaki; Yokosuka, Osamu

doi:10.3892/etm.2017.4407

Cited by 19 publications

(27 citation statements)

References 27 publications

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“…We observed that HAV HA11-1299 genotype IIIA strain replication is enhanced by the accumulation of lipids or high-concentration glucose in the human hepatoma cell line, Huh7 [ 26 , 27 , 28 ]. Hyperglycemia or the accumulation of lipids induces an endoplasmic reticulum (ER) stress response in human hepatocytes.…”

Section: Hav Infection and Metabolic Associated Fatty Liver Diseasmentioning

confidence: 99%

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Co-Occurrence of Hepatitis A Infection and Chronic Liver Disease

Sasaki

Masuzaki

Takahashi

et al. 2020

IJMS

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Hepatitis A virus (HAV) infection occasionally leads to a critical condition in patients with or without chronic liver diseases. Acute-on-chronic liver disease includes acute-on-chronic liver failure (ACLF) and non-ACLF. In this review, we searched the literature concerning the association between HAV infection and chronic liver diseases in PubMed. Chronic liver diseases, such as metabolic associated fatty liver disease and alcoholic liver disease, coinfection with other viruses, and host genetic factors may be associated with severe hepatitis A. It is important to understand these conditions and mechanisms. There may be no etiological correlation between liver failure and HAV infection, but there is an association between the level of chronic liver damage and the severity of acute-on-chronic liver disease. While the application of an HAV vaccination is important for preventing HAV infection, the development of antivirals against HAV may be important for preventing the development of ACLF with HAV infection as an acute insult. The latter is all the more urgent given that the lives of patients with HAV infection and a chronic liver disease of another etiology may be at immediate risk.

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Section: Hav Infection and Metabolic Associated Fatty Liver Diseasmentioning

confidence: 99%

“…GRP78 is a negative regulator of ER stress response. We also observed that the overexpression of GRP78 could inhibit HAV replication, while the knockdown or knockout of GRP78 enhanced HAV replication [ 26 , 28 ]. In sum, GRP78 is an antiviral protein against HAV replication [ 28 ].…”

Section: Hav Infection and Metabolic Associated Fatty Liver Diseasmentioning

confidence: 99%

Co-Occurrence of Hepatitis A Infection and Chronic Liver Disease

Sasaki

Masuzaki

Takahashi

et al. 2020

IJMS

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“…The cellular proteins SAMD9 and WDR6 form host restriction factor that prevent VACV replication in human cells [ 144 ]. CRISPR/Cas9-mediated mutation of the gene for glucose-regulated protein 78 (GRP78), an endoplasmic reticulum chaperone, enhanced hepatitis A virus replication [ 93 ].…”

Section: Applications Of Crispr/cas9 In Genome Editing Of Opxvs Anmentioning

confidence: 99%

CRISPR/Cas9—Advancing Orthopoxvirus Genome Editing for Vaccine and Vector Development

Okoli

Okeke

Tryland

et al. 2018

Viruses

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The clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 (Cas9) technology is revolutionizing genome editing approaches. Its high efficiency, specificity, versatility, flexibility, simplicity and low cost have made the CRISPR/Cas9 system preferable to other guided site-specific nuclease-based systems such as TALENs (Transcription Activator-like Effector Nucleases) and ZFNs (Zinc Finger Nucleases) in genome editing of viruses. CRISPR/Cas9 is presently being applied in constructing viral mutants, preventing virus infections, eradicating proviral DNA, and inhibiting viral replication in infected cells. The successful adaptation of CRISPR/Cas9 to editing the genome of Vaccinia virus paves the way for its application in editing other vaccine/vector-relevant orthopoxvirus (OPXV) strains. Thus, CRISPR/Cas9 can be used to resolve some of the major hindrances to the development of OPXV-based recombinant vaccines and vectors, including sub-optimal immunogenicity; transgene and genome instability; reversion of attenuation; potential of spread of transgenes to wildtype strains and close contacts, which are important biosafety and risk assessment considerations. In this article, we review the published literature on the application of CRISPR/Cas9 in virus genome editing and discuss the potentials of CRISPR/Cas9 in advancing OPXV-based recombinant vaccines and vectors. We also discuss the application of CRISPR/Cas9 in combating viruses of clinical relevance, the limitations of CRISPR/Cas9 and the current strategies to overcome them.

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“…We previously observed that HAV replication was associated with a reduction in glucose-regulated protein 78 (GRP78) expression 9 and reported that GRP78 is an antiviral against HAV infection 10 . Miso is a traditional Japanese seasoning and is used for miso soup, a Japanese culinary staple.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of Japanese rice-koji miso extracts on hepatitis A virus replication in association with the elevation of glucose-regulated protein 78 expression

et al. 2018

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Hepatitis A virus (HAV) infection is one of the major causes of acute hepatitis and acute liver failure in developing and developed countries. Although effective vaccines for HAV infection are available, outbreaks of HAV infection still cause deaths, even in developed countries. One approach to control HAV infection is prevention through diet, which can inhibit HAV propagation and replication. Glucose-regulated protein 78 (GRP78) is a member of the heat shock protein 70 family of molecular chaperone required for endoplasmic reticulum stress and stress-induced autophagy. We previously showed that the elevation of GRP78 expression inhibits HAV replication. It has been reported that Japanese miso extracts, which was made from rice-koji, enhance GRP78 expression. In the present study, we used human hepatoma Huh7 cells and human hepatocyte PXB cells to examine the efficacy of Japanese miso extracts as antiviral agents against HAV. Japanese miso extracts enhanced GRP78 expression and inhibited HAV replication in human hepatocytes. Together, these results demonstrate that Japanese miso extracts may partly modulate GRP78 expression and additively or synergistically work as antivirals against HAV infection. Japanese miso extracts can be used as effective dietary supplements for severe hepatitis A.

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Glucose-regulated protein 78 is an antiviral against hepatitis A virus replication

Cited by 19 publications

References 27 publications

Co-Occurrence of Hepatitis A Infection and Chronic Liver Disease

Co-Occurrence of Hepatitis A Infection and Chronic Liver Disease

CRISPR/Cas9—Advancing Orthopoxvirus Genome Editing for Vaccine and Vector Development

Inhibitory effect of Japanese rice-koji miso extracts on hepatitis A virus replication in association with the elevation of glucose-regulated protein 78 expression

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