Glucose-sensitive field-effect transistor with a membrane containing co-immobilized gluconolactonase and glucose oxidase

Hanazato, Yoshio; Inatomi, Ken-ichi; Nakako, Mamiko; Shiono, Satoru; Maeda, Mitsuo

doi:10.1016/s0003-2670(00)84128-8

Cited by 35 publications

(6 citation statements)

References 17 publications

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“…With H 2 O availability in the reaction, gluconolactone is spontaneously converted to gluconic acid (by hydrolysis reaction), resulting in a decrease of pH was used for the indirect determination of the glucose concentration with charged Typical biosensors usually measure the amount of hydrogen peroxide produced by the reaction between glucose and GO. Alternatively, some sensors (including our biosensor) measure the amount of oxygen consumed, or a change in pH which is accompanied by the production of gluconic acid that is produced from gluconolactone show in equation 2 [38][39][40].…”

Section: Resultsmentioning

confidence: 99%

Needle-Type Glucose Sensor Based on Functionalized Graphene

Hasan¹,

Asif²

2012

J Biosens Bioelectron

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We demonstrate a novel, highly efficient glucose sensor based on functionalized graphene. Glucose oxidase (GOD) immobilization has been apprehendedbythe direct interaction between carboxyl acid groups of the reduced graphene oxide (RGO) and amines of GOD together with the electrostatic interactions existing between the positively charged polymeric ionic liquid (PIL) and GOD. This combined system can provide a favorable microenvironment for the GOD to retain its good bioactivity. The enzyme-coated graphene biosensor exhibited glucose-dependent electrochemical measurements against an Ag/AgCl reference electrode. The resulting sensor show broad range detection, up to 100 mM glucose concentration, with a sensitivity of 5.59 µA/ decade. It was found that glucose biosensor based on functionalized graphene can be seen as an effective candidate for the detection of sugar concentration in clinical diagnoses.

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Section: Resultsmentioning

confidence: 99%

Needle-Type Glucose Sensor Based on Functionalized Graphene

Hasan¹,

Asif²

2012

J Biosens Bioelectron

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“…It has been reported that by incorporating catalase into the GOD immobilized hydrogel, as much as 50% of the oxygen used in converting glucose to gluconic acid can be recovered (Zhang and Wu, 2002). Additionally, it has been suggested that gluconolactonase may be used to increase the rate that gluconic acid is formed, converting the gluconolactone intermediate to gluconic acid (Suzuki and Kumagai, 2003;Hanazato et al, 1988;Ogawa et al, 2002). See Table 1.6 for a compilation of hydrogel systems utilizing GOD in the presence or lack of catalase.…”

Section: Concanavalin a And Allyl Glucose Hydrogel Systemsmentioning

confidence: 99%