Glucosinolate Pattern in<i>Isatis tinctoria</i>and<i>I. indigotica</i>Seeds

Mohn, Tobias; Hamburger, Matthias

doi:10.1055/s-2008-1074554

Cited by 23 publications

(5 citation statements)

References 20 publications

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“…The glucosinolate pattern of the seeds extract showed differences when compared to the extract obtained from the rosette leaves. In fact, the extracts obtained from I. tinctoria frozen and liophylized rosette leaves, mainly contained indolic glucosinolates including glucobrassicin, neoglucobrassicin, sulfoglucobrassicin, and glucotropaeolin ( Figure 7) [49,50]. The analysis carried out by the same authors also showed that the glucosinolate content in the leaf samples was higher than that of the seed samples.…”

Section: Glucosinolatesmentioning

confidence: 91%

Isatis tinctoria L. (Woad): A Review of Its Botany, Ethnobotanical Uses, Phytochemistry, Biological Activities, and Biotechnological Studies

Speranza

Miceli

Taviano

et al. 2020

Plants

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Isatis tinctoria L. (Brassicaceae), which is commonly known as woad, is a species with an ancient and well-documented history as an indigo dye and medicinal plant. Currently, I. tinctoria is utilized more often as medicinal remedy and also as a cosmetic ingredient. In 2011, I. tinctoria root was accepted in the official European phytotherapy by introducing its monograph in the European Pharmacopoeia. The biological properties of raw material have been known from Traditional Chinese Medicine (TCM). Over recent decades, I. tinctoria has been investigated both from a phytochemical and a biological point of view. The modern in vitro and in vivo scientific studies proved anti-inflammatory, anti-tumour, antimicrobial, antiviral, analgesic, and antioxidant activities. The phytochemical composition of I. tinctoria has been thoroughly investigated and the plant was proven to contain many valuable biologically active compounds, including several alkaloids, among which tryptanthrin, indirubin, indolinone, phenolic compounds, and polysaccharides as well as glucosinolates, carotenoids, volatile constituents, and fatty acids. This article provides a general botanical and ethnobotanical overview that summarizes the up-to-date knowledge on the phytochemistry and biological properties of this valuable plant in order to support its therapeutic potential. Moreover, the biotechnological studies on I. tinctoria, which mainly focused on hairy root cultures for the enhanced production of flavonoids and alkaloids as well as on the establishment of shoot cultures and micropropagation protocols, were reviewed. They provide input for future research prospects.

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Section: Glucosinolatesmentioning

confidence: 91%

Isatis tinctoria L. (Woad): A Review of Its Botany, Ethnobotanical Uses, Phytochemistry, Biological Activities, and Biotechnological Studies

Speranza

Miceli

Taviano

et al. 2020

Plants

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“…Given our ongoing studies on indigoferous Isatis species [7][8][9][10], the draft monograph, and in particular the HPLC assay, drew our attention because of the extremely poor solubility of indigo. Another point that needed some investigation was the fact that > 97% of this drug remained undefined.…”

Section: Fidmentioning

confidence: 99%

Composition ofIndigo naturalis

Plitzko¹,

Mohn²,

Sedlacek³

et al. 2009

Planta Med

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A proposal for a European Pharmacopoeia monograph concerning Indigo naturalis has recently been published, whereby the indigo (1) and indirubin (2) content should be determined by HPLC-UV. This method was tested, but problems were seen with the dosage of indigo due to poor solubility. A quantitative assay for indigo based on (1)H-NMR was developed as an alternative. The HPLC and qNMR assays were compared with eight Indigo naturalis samples. The HPLC assay consistently gave much lower indigo concentrations because solubility was the limiting factor in sample preparation. In one sample, sucrose was identified by (1)H-NMR as an organic additive. Simple wet chemistry assays for undeclared additives such as sugars and starch were tested with artificially spiked Indigo naturalis samples to establish their limits of detection, and sulfate ash determinations were carried out in view of a better assessment of Indigo naturalis in a future European monograph.

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“…Over the last decades, more than 100 bioactive constituents have been identified in methanolic extracts of I. tinctoria leaves and roots, including numerous alkaloids, fatty acids, and carotenoids (Mohn et al, 2009), as well as phenylpropanoid derivatives, such as flavonoids, hydroxyl‐cinnamic and hydroxyl‐benzoic acid derivatives, or mono‐ and oligo‐lignols (Nguyen et al, 2017). Especially, the glucosinolates, indole‐derived compounds, have attracted interest for their prospective medical properties (Mohn and Hamburger, 2008; Nguyen et al, 2017). For instance, indirubin, a bis‐indole alkaloid, was reported to mitigate expression of the influenza infection marker RANTES (regulated on activation, normal T cell expressed and secreted) in a bronchitis cell model (Mak et al, 2004; Sethi et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Anti‐microtubule activity of the traditional Chinese medicine herb Northern Ban Lan (Isatis tinctoria) leads to glucobrassicin

Guan

Zhou

Girel

et al. 2021

JIPB

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Traditional Chinese medicine (TCM) belongs to the most elaborate and extensive systems of plant-based healing. The herb Northern Ban Lan (Isatis tinctoria) is famous for its antiviral and anti-inflammatory activity. Although numerous components isolated from I. tinctoria have been characterized so far, their modes of action have remained unclear. Here, we show that extracts from I. tinctoria exert antimicrotubular activity. Using time-lapse microscopy in living tobacco BY-2 (Nicotiana tabacum L. cv Bright Yellow 2) cells expressing green fluorescent protein-tubulin, we use activity-guided fractionation to screen out the biologically active compounds of I. tinctoria. Among 54 fractions obtained from either leaves or roots of I. tinctoria by methanol (MeOH/H 2 O 8:2), or ethyl acetate extraction, one specific methanolic root fraction was selected, because it efficiently and rapidly eliminated microtubules. By combination of further purification with ultrahigh-performance liquid chromatography and high-resolution tandem mass spectrometry most of the bioactivity could be assigned to the glucosinolate compound glucobrassicin. Glucobrassicin can also affect microtubules and induce apoptosis in HeLa cells. In the light of these findings, the antiviral activity of Northern Ban Lan is discussed in the context of microtubules being hijacked by many viral pathogens for cell-to-cell spread.

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Glucosinolate Pattern inIsatis tinctoriaandI. indigoticaSeeds

Cited by 23 publications

References 20 publications

Isatis tinctoria L. (Woad): A Review of Its Botany, Ethnobotanical Uses, Phytochemistry, Biological Activities, and Biotechnological Studies

Isatis tinctoria L. (Woad): A Review of Its Botany, Ethnobotanical Uses, Phytochemistry, Biological Activities, and Biotechnological Studies

Composition ofIndigo naturalis

Anti‐microtubule activity of the traditional Chinese medicine herb Northern Ban Lan (Isatis tinctoria) leads to glucobrassicin

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