2010
DOI: 10.1007/s10529-010-0244-6
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Glutathione-mediated response to acid stress in the probiotic bacterium, Lactobacillus salivarius

Abstract: Lactobacillus salivarius, a probiotic bacterium, encounters acidic conditions in its passage through the gastrointestinal tract of human and animal hosts. We studied the effect of a rapid downshift in extracellular pH from 6.5 to 4 on cell growth. The maximum growth rate was higher in low pH medium with glutathione supplementation than without. Cells developed a GSH-mediated acid-tolerance response and, when grown with 0.5 mM GSH, reached a higher final density than with other conditions. These findings sugges… Show more

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Cited by 24 publications
(15 citation statements)
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“…Similarly, GSH synthesis is being presumptively shown in Lb. salivarius [40,41]. However, the genetic elements associated with the GSH system in Lb.…”
Section: Distribution Of Glutathione In Selected Lab and Closely Relamentioning
confidence: 99%
See 1 more Smart Citation
“…Similarly, GSH synthesis is being presumptively shown in Lb. salivarius [40,41]. However, the genetic elements associated with the GSH system in Lb.…”
Section: Distribution Of Glutathione In Selected Lab and Closely Relamentioning
confidence: 99%
“…The latter mechanism is also supported by the finding that growth rate was enhanced post GSH supplementation in Lb. salivarius cells under acid stress [40]. Synthesized GSH also offered protection against acid stress in genetically engineered strain of Lc.…”
Section: Role Of Glutathione In Food Grade Labmentioning
confidence: 99%
“…There is at least one Lactobacillus strain under investigation with local (gastrointestinal) beneficial effects presumably due to its release of GSH [48]. The synthesis of GSH by Lactobacilli is not a common property [13]. One approach is developing a microbe [24,49] that can induce GSH synthesis in humans, but biotechnological products have still some unsolved issues.…”
Section: Discussionmentioning
confidence: 99%
“…The latter is important as without GSH and the glutathione redox cycle, LAB cannot effectively protect themselves against reactive oxygen species (oxidative stress) as the antioxidative potency in lactobacilli has a significant positive correlation with cellular GSH levels [11,12]. At the same time, the ability to synthesize GSH is rare in LAB [13]. Glutathione (L-gamma-Glu-L-Cys-Gly or GSH) is a major cellular non-enzymatic antioxidant.…”
mentioning
confidence: 99%
“…It is present in most living cells but absent from most Gram-positive bacteria because of a lack of the cglutamylcysteine synthetase (gshA) and glutathione synthetase (gshB) genes or gshA-gshB fused form (gshF) required for GSH synthesis (Lee et al 2010). The genomic analysis of ATCC 8293 revealed the presence of a glutathione peroxidase gene (LEUM_0007).…”
Section: Discussionmentioning
confidence: 99%